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Showing: 4201 - 4250 of 9181 results

  10. pHL 1035

    Plasmid
    #52999
    Purpose
    pLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    fhlA
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains −309 to +96 bp relative to start codon a…
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pHL 1033

    Plasmid
    #52998
    Purpose
    pLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    rpoS
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains −150 and +30 bp relative to start codon …
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. BirA in pET28a (w400-2)

    Plasmid
    #26624
    Depositor
    Eric Campeau
    Insert
    BirA
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    26624-plasmid-map-sequence-id-228306

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  17. pOP3SU

    Plasmid
    #112599
    Purpose
    Used for expression of protein in E. coli as N-terminal Sumo tag fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Sumo
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112599-plasmid-map-sequence-id-220209

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  18. pSpot2

    Plasmid
    #117063
    Purpose
    For expression of proteins with C-terminal Spot-Tag in E. coli
    Depositor
    Felix Hartlepp
    Type
    Empty backbone
    Use
    Tags
    Spot-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    117063-plasmid-map-sequence-id-226326

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  19. pOP2H

    Plasmid
    #112596
    Purpose
    Used for expression of protein in E. coli as non-cleavable C-terminal 8His-tag fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112596-plasmid-map-sequence-id-220202

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  20. pTar-ipgC

    Plasmid
    #97097
    Purpose
    Expresses ipgC in the presence of E. coli sigma factor ?28
    Depositor
    Richard Murray
    Insert
    IpgC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTar
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97097-plasmid-map-sequence-id-182496

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  21. pDest-557

    Plasmid
    #161890
    Purpose
    Standard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions in E. coli
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    161890-plasmid-map-sequence-id-322136

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  22. pCDF-mcherry2

    Plasmid
    #87145
    Purpose
    Constitutive E. coli mcherry Expression, with DAS4 tag
    Depositor
    Michael Lynch
    Insert
    mcherry
    Use
    Tags
    C terminal DAS4 tag
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive proD
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87145-plasmid-map-sequence-id-176838

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  23. pOP2S

    Plasmid
    #112597
    Purpose
    Used for expression of protein in E. coli as non-cleavable C-terminal Strep Tag fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112597-plasmid-map-sequence-id-220203

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  24. pAMHDAdGT8-4

    Plasmid
    #26421
    Depositor
    Kohnosuke Mitani
    Insert
    HSV-TK, mCMV-b-gal
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2011
    Availability
    Academic Institutions and Nonprofits only
    26421-plasmid-map-sequence-id-12467

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  25. pCIHDAdGT8-3

    Plasmid
    #26525
    Depositor
    Kohnosuke Mitani
    Insert
    HSV-TK, mCMV-b-gal
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 2, 2011
    Availability
    Academic Institutions and Nonprofits only
    26525-plasmid-map-sequence-id-12551

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  26. pTar-deGFP

    Plasmid
    #97098
    Purpose
    Expresses GFP in the presence of E. coli sigma factor ?28
    Depositor
    Richard Murray
    Insert
    deGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTar
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    97098-plasmid-map-sequence-id-182495

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  29. pSAM_AraC

    Plasmid
    #91569
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Insert
    AraC/PBAD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91569-plasmid-map-sequence-id-181631

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  31. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid
    #169658
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169658-plasmid-map-sequence-id-365631

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  33. pEM-Cas9HF1

    Plasmid
    #89961
    Purpose
    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
    Depositor
    Michael Lynch
    Article
    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021
    Insert
    Cas9HF1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N497A/R661A/Q695A/Q926A
    Promoter
    pTet
    Available since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    89961-plasmid-map-sequence-id-181339

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  40. pCasTet-λ

    Plasmid
    #128318
    Purpose
    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.
    Depositor
    Christopher Johnston
    Article
    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p
    Insert
    TetR and pTetR/TetO
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Constitutive expression of cas9 and anhydro-tetra…
    Promoter
    tet promoter
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128318-plasmid-map-sequence-id-254797

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  42. Tn7 integration plasmid

    Plasmid
    #154134
    Purpose
    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.
    Depositor
    Shimon Bershtein
    Article
    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038
    Inserts
    left and right end of Tn7 arm
    Spectinomycin resistant gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Spectinomycin resistance gene promoter and no
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154134-plasmid-map-sequence-id-306811

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  47. nuc-GafD-mTurboID-HA

    Plasmid
    #184641
    Purpose
    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    nuc-GlycoID-HA
    Use
    Tags
    3xHA tag and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    GafD -> expressed 22-178 ; BirA* -> express…
    Promoter
    CMV
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184641-plasmid-map-sequence-id-366747

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  49. cyt-GafD-mTurboID-V5

    Plasmid
    #184640
    Purpose
    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    cyt-GlycoID-V5
    Use
    Tags
    Nuclear export sequence and V5 tag
    Expression
    Mammalian
    Mutation
    GafD -> expressed aa22-178 ; BirA* -> expre…
    Promoter
    CMV
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184640-plasmid-map-sequence-id-366751

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Showing: 4201 - 4250 of 9181 results