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We narrowed to 9,455 results for: Coli

Showing: 4201 - 4250 of 9455 results

  2. pSLS.546

    Plasmid
    #184963
    Purpose
    Test effect of a1/a2 length on editing priB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
    Expression
    Bacterial
    Mutation
    priB donor G1069A, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184963-plasmid-map-sequence-id-376688

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  3. HpMscS

    Plasmid
    #78310
    Purpose
    H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag
    Depositor
    Douglas Rees
    Article
    Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:
    Insert
    Mechanosensitive channel of small conductance
    Tags
    6x His Tag and FLAG Tag
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    78310-plasmid-map-sequence-id-151301

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  5. pRB113.2

    Plasmid
    #181948
    Purpose
    aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    NarL-mNG; mCherry; tetR (AX04_RS23485, narL Synthetic, narL - E. coli)
    Tags
    mNeonGreen
    Expression
    Bacterial
    Promoter
    NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181948-plasmid-map-sequence-id-356574

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  6. pIBA2-SpyTag-MBP

    Plasmid
    #107422
    Purpose
    Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    Maltose-binding protein (MalE)
    Tags
    OmpA signal peptide, SpyTag, and StrepII
    Expression
    Bacterial
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107422-plasmid-map-sequence-id-212955

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  12. pSLS.540

    Plasmid
    #184959
    Purpose
    Test effect of a1/a2 length on editing fabH using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
    Expression
    Bacterial
    Mutation
    fabH donor G954A, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184959-plasmid-map-sequence-id-376675

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  14. pCarCBA

    Plasmid
    #117754
    Purpose
    Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
    Expression
    Bacterial
    Promoter
    PLac(UV5)
    Available Since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117754-plasmid-map-sequence-id-229023

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  15. p158

    Plasmid
    #62617
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62617-plasmid-map-sequence-id-102961

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  18. p15aC-3D1D-5

    Plasmid
    #121050
    Purpose
    Bacterial expression of PIS, PI4K and PI3P5K mutant E545K
    Depositor
    Sanford Simon
    Article
    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:
    Inserts
    PIS
    phosphatidylinositol 4-phosphate 5-kinase
    phosphatidylinositol 3-kinase catalytic subunit p110α of class IA E545K
    Tags
    myc and none
    Expression
    Bacterial
    Available Since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121050-plasmid-map-sequence-id-237447

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  23. pJM149

    Plasmid
    #134168
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Article
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_SSSC E118A R129A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC E118A R129A, E. coli codon optimized, CRISPR…
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    134168-plasmid-map-sequence-id-271754

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  24. pJM147

    Plasmid
    #134167
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Article
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_SSSC E47A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC E47A, E. coli codon optimized, CRISPR/Cas9 h…
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    134167-plasmid-map-sequence-id-271753

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  25. pCarCBAE

    Plasmid
    #117755
    Purpose
    Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
    Expression
    Bacterial
    Promoter
    PLac(UV5)
    Available Since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117755-plasmid-map-sequence-id-229025

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  27. pBP-ORF

    Plasmid
    #72949
    Purpose
    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - ORFs
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72949-plasmid-map-sequence-id-140106

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  35. pAL950

    Plasmid
    #119748
    Purpose
    precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    Tags
    none
    Expression
    Bacterial
    Mutation
    Internal NdeI site removed and mutations Alanine …
    Promoter
    T7
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119748-plasmid-map-sequence-id-232481

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  40. pLaNGo11-3

    Plasmid
    #126054
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126054-plasmid-map-sequence-id-254921

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  41. pMiNGo11-1

    Plasmid
    #126056
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126056-plasmid-map-sequence-id-246974

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  48. pBA1729

    Plasmid
    #246063
    Purpose
    Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a ori
    Depositor
    Jennifer Doudna
    Article
    Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2
    Insert
    Panoptes locus
    Expression
    Bacterial
    Promoter
    pTet promoter
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    246063-plasmid-map-sequence-id-490799

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  50. pYihI-C-term K-R

    Plasmid
    #233092
    Purpose
    Expression of GST-YihI with C-term lysines (K) mutated to arginine (R)
    Depositor
    Michael Downey
    Article
    Baijal et al bioRxiv 2025.02.12.637445
    Insert
    GST-YihI with C-term lysines (K) mutated to arginine (R)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    C-term lysines (K) mutated to arginine (R)
    Available Since
    May 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233092-plasmid-map-sequence-id-472234

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Showing: 4201 - 4250 of 9455 results