We narrowed to 7,491 results for: RAP
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Plasmid#216058PurposeFragmid fragment: (2A Selection) Puromycin resistance, T2A, EGFPDepositorHas ServiceCloning Grade DNAInsertP2A_v1.1; PuroR_v1.1; T2A_v1.2; EGFP_v1.1; Stop
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA176
Plasmid#216063PurposeFragmid fragment: (2A Selection) mCherryDepositorHas ServiceCloning Grade DNAInsertP2A_v1.1; mCherry_v1.1; Stop
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTK0_015
Plasmid#123930PurposeEncodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertPB Destination - Insulator + tagBFP
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR eGFP L5-L2
Plasmid#62183PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing EGFP. Compatible with MultiSite Gateway cloningDepositorInserteGFP
UseMule gateway entry vectorExpressionMammalianAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_synRobo/Notch
Plasmid#188341PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Robo1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_Robo1-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/M1 S245X
Plasmid#92366Purposeexpression of M1 S245X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
ExpressionMammalianMutation1-194 bp of 5'UTR deleted, S245XPromoterCMVAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXb3
Plasmid#73615PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXb3
TagsmCherryExpressionBacterial, Insect, and Mamm…Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-GBP
Plasmid#67672PurposeMultisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)DepositorInsertanti-GFP nanobody
UseGateway multisite 3' entry cloneAvailable SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK3_009
Plasmid#123724PurposeEncodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK systemDepositorInsertPHYB-VP16-NLS
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR MCS L1-R5
Plasmid#62084PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseMule gateway entry vectorExpressionMammalianAvailable SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMGS59(AID-ARF-P2A-Hygromycin)
Plasmid#138174PurposeAn AID-ARF-P2A-Hygromycin fusion cassette to tag the protein of interests with auxin-inducible degron and ARF at the c-terminalDepositorInsertAID-ARF-P2A-Hygromycin
TagsAID-ARF clamp degron tagAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SP-CLSTN2-3xHAtag
Plasmid#191693PurposeFor expression coding sequencing of CLSTN2DepositorInsertsignal peptide, full length coding sequence of CLSTN2 and 3xHAtag
ExpressionMammalianMutationWTAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
ST-SBP-EGFP_puromycin
Plasmid#65270PurposeST reporter only ( no hook) (RUSH system)DepositorInserttruncated sialyltransferase (ST6GAL1 Human)
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC(S75N)
Plasmid#99645PurposeBacterial expression of RagA-RagC(S75N)DepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-RFC5
Plasmid#183200PurposeEntry cloneDepositorInsertCas9-RFC5
UseCRISPR; Gateway entry cloneMutationn/aAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Notch1 del-NRR
Plasmid#188353PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-del-NRR-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_Notch1-NRRdeletion-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEG R1-ChlorR-R4 #BGV049
Plasmid#48943PurposeContains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectorsDepositorTypeEmpty backboneUseGateway cloning vectorPromoternaAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP
Plasmid#199361PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOstar-EGFPExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP
Plasmid#199362PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOstar-EGFPExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag 3B/WT myc-M87
Plasmid#87722PurposeExpresses WT myc-M87 spastin isoform in mammalian cellsDepositorInsertSPAST (SPAST Human)
TagsMycExpressionMammalianMutationM87 isoform (deletion of nt 1-490 - 5'UTR an…PromoterCMVAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6-miR-30 L1-R5
Plasmid#62113PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorExpressionMammalianAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag RagB
Plasmid#112755Purposeexpress Flag-RagBDepositorAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCIPpay-BFP reporter
Plasmid#154096PurposeSelf-Cutting and Integrating Payload CRISPR backbone with BFP reporterDepositorInsertP2A-tagBFP
UseCRISPRExpressionMammalianAvailable SinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP8GF-AtTIR1
Plasmid#188427PurposeArabidopsis Nuclear Auxin Signaling Receptor TIR1DepositorInsertAuxin Signaling Receptor TIR1 (TIR1 Mustard Weed)
ExpressionYeastAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P1-N-miRFP670-C
Plasmid#159438PurposeCRISPR knock-in donor construct with fluorescent reporterDepositorInsertmiRFP670
UseCRISPRTags3' linker pair 1, 5' linker pair 1, C t…PromoterCMVAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL704
Plasmid#140374PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoterDepositorInsertT7-4BP-tetO-3WJdB-T
UseSynthetic BiologyAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pREG(R1-R3) #BGV141
Plasmid#48958PurposeRetroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).DepositorTypeEmpty backboneUseRetroviralPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-tagBFP
Plasmid#65254Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
TagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
FFiBlast MSCV Puro
Plasmid#68464PurposeRetroviral plasmid for establishment of blasticidin resistance Cre-reporter cell lineDepositorInsertBlasticidin Resistance
UseCre/Lox and RetroviralExpressionMammalianMutationInvertedPromoterLTRAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spy)
Plasmid#133316Purposeexpresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pyogenes)
UseCRISPRPromoterxyloseAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mGFP-APEX2
Plasmid#171936PurposeReporter for correlated light and electron microscopy.DepositorInsertmGFP-APEX2
UseAAV and Cre/LoxExpressionMammalianPromoterCAGAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-27
Plasmid#172752PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-27
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P1-N-EGFP-C
Plasmid#159436PurposeCRISPR knock-in donor construct with fluorescent reporterDepositorInsertEGFP
UseCRISPRTags3' linker pair 1, 5' linker pair 1, C t…PromoterCMVAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
E0030 (YFP)_CD
Plasmid#66031PurposeMoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]DepositorInsertFluorescent reporter - YFP
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJBL705
Plasmid#140375PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoterDepositorInsertT7-5BP-tetO-3WJdB-T
UseSynthetic BiologyAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSK260
Plasmid#129252Purposedual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporterDepositorInsertSup35 NM yTRAP sensor reporting on mKate2
ExpressionYeastAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/M87 S245X
Plasmid#92368Purposeexpression of M87 S245X spastin in mammalian cellsDepositorAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP
Plasmid#192609PurposeLentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.DepositorInsertLexA-CIB1-biLINuS-P2A-tagBFP
UseLentiviral and Synthetic BiologyTagstagBFPPromoterPGKAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iCreT2ALuc-L3 #BGV208
Plasmid#48992PurposeSupplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertscre recombinase
Luciferase
UseLuciferase; Gateway cloning vectorTagsIRES (weak)PromoterUnknownAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCheck2 Dest (R1-R2) #BGV129
Plasmid#48955PurposeDestination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.DepositorTypeEmpty backboneUseLuciferase; Gateway cloning vectorPromoterSV40Available SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGK:HygroR-CMV:mKO2-C_UTR
Plasmid#68481PurposeLentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistanceDepositorInsertsHygromycin Resistance
mKO2-C_UTR
UseLentiviralExpressionMammalianMutationshRNA sensor cassette "B" added to 3…PromoterCMV and pGKAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLN431 (FuGW-G8p-mKate2-1Pe )
Plasmid#105186PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)DepositorInsertG8p-mKate2-1Pe
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.8
Plasmid#133521PurposesfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start siteDepositorInsertsfGFP
TagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available SinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY273
Plasmid#184155PurposeReprogrammed short tracrRNA for hijacking mRNA of zraPDepositorInserts-tracrRNA-zraP-22
UseSynthetic BiologyExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta11 Aphrodite
Plasmid#13569DepositorInsertFLIPglu F16A Delta11 Aphrodite
TagsAphrodite and ECFPExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…Available SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
CTA2-mNG211
Plasmid#141167PurposeExpresses GST-SUMO tagged CTA2 Subunit with 11th StrandDepositorInsertSUMO-CTA2-mNG211
TagsSUMOExpressionBacterialAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA pDEST40 Cas9 GW STOP
Plasmid#183196PurposeDestination vectorDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-8
Plasmid#172761PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-8
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only