4,073 results

1. SNX9-pmCherryC1

   Plasmid

   #27678

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   SNX9 (Snx9 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMJ841

   Plasmid

   #39318

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A) and Histidine 840 …

   Promoter

   T7

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVCT-rtTR-KRAB-2SM2

   Plasmid

   #11779

   Purpose

   Tet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   CAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBabe K-Ras 12V

   Plasmid

   #12544

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   K-Ras 12V (KRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   12V constituitively activated

   Promoter

   Available since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLQ1661-sgMUC4-E3(F+E)

   Plasmid

   #51025

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

   Depositor

   Stanley Qi, Bo Huang

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   optimized sgRNA (MUC4 Mouse, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBABE puro PPAR gamma 2

   Plasmid

   #8859

   Depositor

   Bruce Spiegelman

   Article

   Tontonoz et al Cell. 1994 Dec 30. 79(7):1147-56.

   Insert

   PPAR gamma (Pparg Mouse)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-FLAG LAP2

   Plasmid

   #15738

   Depositor

   Joan Massague

   Article

   Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

   Insert

   LAP2 (CEBPB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVUHshp53

   Plasmid

   #11653

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   GFP, shRNA against TP53 (TP53 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLM-mCerulean-cMyc

   Plasmid

   #23244

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

   Insert

   mCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Stat1 alpha Flag pRc/CMV

    Plasmid

    #8691

    Depositor

    Jim Darnell

    Article

    Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

    Insert

    STAT1 alpha (STAT1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS hShh (CT#401)

    Plasmid

    #13996

    Depositor

    Cliff Tabin

    Article

    Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

    Insert

    Shh (SHH Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-TXNIP

    Plasmid

    #18758

    Depositor

    Clark Distelhorst

    Article

    Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

    Insert

    thioredoxin-interacting protein (Txnip Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-HA::UPRT

    Plasmid

    #47110

    Purpose

    for subcloning UPRT into expression vectors

    Depositor

    Chris Doe, Mike Cleary

    Article

    Miller et al Nat Methods. 2009 Jun;6(6):439-41. doi:

    Insert

    uracil phosphoribosyltransferase
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PL552

    Plasmid

    #68407

    Purpose

    Donor empty vector with floxed puromycin resistance gene expression cassette

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse PGK promoter

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVPT-tTR-KRAB

    Plasmid

    #11642

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT3-EF1a-Bmi1

    Plasmid

    #31783

    Depositor

    Xin Chen

    Article

    Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

    Insert

    Bmi1 (BMI1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO-RB1-shRNA19

    Plasmid

    #25640

    Purpose

    3rd generation lentiviral vector

    Depositor

    Todd Waldman

    Article

    Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

    Insert

    RB1-shRNA 19 (RB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCE-BiFC-VC155

    Plasmid

    #22020

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Nat Protoc. 2008 . 3(4):588-96.

    Insert

    VC155
    

    Use

    Tags

    HA

    Expression

    Worm

    Mutation

    Changed Alanine 206 to Lysine

    Promoter

    Available since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEYFP SUMO-1

    Plasmid

    #13380

    Depositor

    Mary Dasso

    Article

    Ayaydin et al Mol Biol Cell. 2004 Dec . 15(12):5208-18

    Insert

    SUMO-1 (SUMO1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pROSA26-PA

    Plasmid

    #21271

    Depositor

    Frank Costantini

    Article

    Srinivas et al BMC Dev Biol. 2001 . 1():4.

    Insert

    ROSA26 (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51084

    Purpose

    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde and AAV1

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin1

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAMPK alpha2 WT

    Plasmid

    #15991

    Depositor

    Morris Birnbaum

    Article

    Mu et al Mol Cell. 2001 May . 7(5):1085-94.

    Insert

    AMPK alpha2 (Prkaa2 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGFP-Rab8A

    Plasmid

    #24898

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    hRab8A
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p3962 pcDNA3-IRF3-FL

    Plasmid

    #22860

    Depositor

    Peter Howley

    Article

    Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CD3 delta YFP

    Plasmid

    #11951

    Depositor

    Nico Dantuma

    Article

    Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

    Insert

    CD3delta-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLVU/RED

    Plasmid

    #24178

    Depositor

    Lars Ittner

    Article

    Krupka et al Plasmid. 2010 Feb 2. ():.

    Insert

    Chloramphenicol resistance gene (CmR) - ccdB gene
    

    Use

    Lentiviral; Gateway

    Tags

    DsRED

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJ241-FLEX

    Plasmid

    #18925

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    FLEX switch
    

    Use

    Cre/Lox; Subcloning

    Tags

    Expression

    Mutation

    An internal Spe1 site is used for subcloning a ge…

    Promoter

    Available since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Tag5Amyc-GSK3b KD

    Plasmid

    #16262

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    GSK3 beta (GSK3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Kinase dead: K85A

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. M57 pPET28a-TEV-full-length human beta catenin

    Plasmid

    #17198

    Depositor

    Randall Moon

    Insert

    beta catenin (CTNNB1 Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBad-mKalama1

    Plasmid

    #14892

    Purpose

    Bacterial expression of mKalama1 blue fluorescent protein

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 Apr 20. ():.

    Insert

    mKalama1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    The fluorescent protein was inserted between XhoI…

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

31. pWZL Blast GFP

    Plasmid

    #12269

    Depositor

    Bob Weinberg

    Article

    Orimo et al Cell. 2005 May 6. 121(3):335-48.

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Use

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. -962 human cyclin D1 promoter pGL3Basic

    Plasmid

    #32727

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase; Luciferase reporter gene

    Tags

    Expression

    Mutation

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GFP-RB FL

    Plasmid

    #16004

    Depositor

    Olimpia Meucci

    Article

    Khan et al Cell Death Differ. 2008 Oct . 15(10):166

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PCDNA3 Flag p38 alpha (agf)

    Plasmid

    #20352

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Replace Thr 180 and Tyr 182 with Ala and Phe resp…

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDS-RED-C1-Beclin1

    Plasmid

    #24405

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    DsRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDONR223 SARS-CoV-2 NSP6

    Plasmid

    #141260

    Purpose

    Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pFLPo

    Plasmid

    #13792

    Purpose

    Expression of mouse codon-optimized FLP recombinase

    Depositor

    Philippe Soriano

    Article

    Raymond et al PLoS ONE. 2007 . 2():e162.

    Insert

    FLPo
    

    Use

    Cloning vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFRT/TO/HIS/FLAG/HA-ALKBH5

    Plasmid

    #38073

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    ALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    cDNA clone MGC:71143 IMAGE:6339184 with longer 3&…

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLOVE-GFP

    Plasmid

    #15949

    Depositor

    Miguel Ramalho-Santos

    Article

    Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

    Insert

    Green Fluorescent Protein
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    The recombination reaction required to clone &quo…

    Promoter

    Available since

    Nov. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge