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Synthetic Biology

12,047 results

Showing: 4451 - 4500 of 12047 results

  3. DT043A

    Plasmid
    #159404
    Purpose
    Constitutive superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.T7-sfGFP
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7 bacteriophage promoter
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    159404-plasmid-map-sequence-id-324320

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  7. pET-PesaR-Plux

    Plasmid
    #47659
    Purpose
    PesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR)
    Depositor
    Cynthia Collins
    Article
    Shong et al ACS Synth Biol. 2013 Jul 23.
    Insert
    PesaR-P
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Additional esa box downstream of the original esa…
    Promoter
    PesaR-P
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pAAV-EF1-fDIO-mNeon-WPRE-PolyA

    Plasmid
    #121546
    Purpose
    Super-bright fluorescence protein for tracking and visualization
    Depositor
    Matthias Prigge
    Insert
    cre-dependent mNeon
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    121546-plasmid-map-sequence-id-308125

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  9. pCOCK CM-R05

    Plasmid
    #46568
    Purpose
    Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).
    Depositor
    Clifford Wang
    Article
    Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.
    Insert
    CMV mutant promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Mutant version of CMV promoter (refer to Table 1 …
    Promoter
    Available since
    Sept. 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pKB253

    Plasmid
    #170036
    Purpose
    Encodes LacI-controlled expression of surface-displayed K5L7 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    K5L7
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    May 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    170036-plasmid-map-sequence-id-335965

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  20. C27m

    Plasmid
    #120965
    Purpose
    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    tetR (no deg tags)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120965-plasmid-map-sequence-id-236387

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  21. LSB-hsa-miR-99b-5p

    Plasmid
    #103767
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-99b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-99b-5p target (MIR99B Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103767-plasmid-map-sequence-id-208252

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  24. KG802: pMVP (L3-L2) V5 epitope tag + polyA

    Plasmid
    #121755
    Purpose
    pMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    V5 epitope tag + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121755-plasmid-map-sequence-id-237195

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  28. LSB-hsa-miR-495-3p

    Plasmid
    #103568
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-495-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-495-3p target (MIR495 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103568-plasmid-map-sequence-id-196184

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  30. LSB-hsa-miR-125b-5p

    Plasmid
    #103191
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-125b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-125b-5p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103191-plasmid-map-sequence-id-237629

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  31. LSB-hsa-miR-26a-5p

    Plasmid
    #103379
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-26a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-26a-5p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    103379-plasmid-map-sequence-id-195807

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  32. LSB-hsa-miR-26a-1-3p

    Plasmid
    #103377
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-26a-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-26a-1-3p target (MIR26A1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103377-plasmid-map-sequence-id-195803

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  33. LSB-hsa-miR-26a-2-3p

    Plasmid
    #103378
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-26a-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-26a-2-3p target (MIR26A2 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103378-plasmid-map-sequence-id-195805

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  34. C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

    Plasmid
    #162949
    Purpose
    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162949-plasmid-map-sequence-id-330549

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  37. UASgal-A9-Pleum

    Plasmid
    #60343
    Purpose
    Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 6, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    60343-plasmid-map-sequence-id-96621

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  40. pYCTK009

    Plasmid
    #176713
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Eremothecium cymbalariae (sender part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    mfalpha1Ec
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Codon-optimized for expression in S. cerevisiae
    Promoter
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176713-plasmid-map-sequence-id-351598

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  41. pYCTK020

    Plasmid
    #176724
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Eremothecium cymbalariae (receiver part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    ste2Ec
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Codon-optimized for expression in S. cerevisiae
    Promoter
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176724-plasmid-map-sequence-id-351591

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  42. pYCTK030

    Plasmid
    #176734
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Candida albicans (barrier part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    bar1Ca
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Codon-optimized for expression in S. cerevisiae
    Promoter
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176734-plasmid-map-sequence-id-351580

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  43. pYCTK031

    Plasmid
    #176735
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Eremothecium cymbalariae (barrier part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    bar1Ec
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Codon-optimized for expression in S. cerevisiae
    Promoter
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176735-plasmid-map-sequence-id-351578

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  44. pYCTK008

    Plasmid
    #176712
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Candida albicans (sender part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    mfalpha1Ca
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Codon-optimized for expression in S. cerevisiae
    Promoter
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176712-plasmid-map-sequence-id-351558

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  45. pFH88

    Plasmid
    #128201
    Purpose
    sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
    Depositor
    Vladimir Nekrasov
    Article
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128201-plasmid-map-sequence-id-252921

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  47. pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49218
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49218-plasmid-map-sequence-id-73625

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  48. pLY134

    Plasmid
    #130962
    Purpose
    Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB5
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Anderson promoter J23100
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    130962-plasmid-map-sequence-id-257714

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  49. pLY54

    Plasmid
    #130923
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA2B2, and Ptet
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130923-plasmid-map-sequence-id-257307

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Showing: 4451 - 4500 of 12047 results