We narrowed to 18,980 results for: INO

1. pTYF-super-mfCCK-0.5k-Venus-WPRE

   Plasmid

   #156392

   Purpose

   Expresses Venus under CCK gene promoter

   Depositor

   Shuji Kaneko

   Article

   Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

   Inserts

   Venus
   Gal4p65
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pARF6(T27N)-CFP

   Plasmid

   #11386

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   ADP Ribosylation Factor 6 (ARF6 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Changed Threonine 27 to Asparagine.

   Promoter

   Available since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNOC-ARS-CRISPR-v2

   Plasmid

   #99863

   Purpose

   Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Tags

   Expression

   Mutation

   Promoter

   Ribi

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBa.Halo-myosin Va tail 1006-1828

   Plasmid

   #162715

   Purpose

   Mammalian expression of rat WT Myosin Va with deletion of the 1005 N-terminal residues

   Depositor

   Marvin Bentley

   Article

   Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

   Insert

   Myosin Va (Myo5a Rat)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   WT with deletion of the 1005 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSUB11

   Plasmid

   #169699

   Purpose

   Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
   

   Use

   Tags

   3xFLAG tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-CMV-cGASΔN-HA

   Plasmid

   #130912

   Purpose

   Expresses human cGASΔN (aa160-522)-HA; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   cGASΔN
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R26-EGFP HMEJ donor vector

   Plasmid

   #137927

   Purpose

   ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method

   Depositor

   Hiroshi Kiyonari

   Article

   Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

   Insert

   SA-EGFP-bpA with homology arms
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-flag-mKdm4d-polyA

   Plasmid

   #61553

   Purpose

   For mRNA synthesis or expression in mammalian cells

   Depositor

   Yi Zhang

   Article

   Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

   Insert

   Kdm4d (Kdm4d Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 Flag TSC2 (S939A/T1462A)

   Plasmid

   #14131

   Depositor

   Brendan Manning

   Article

   Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

   Insert

   TSC2 S939A/T1462A (TSC2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S939A/T1462A

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pPD_Pinx-1::ChR2(H134R)::mCherry

    Plasmid

    #117420

    Purpose

    For selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoter

    Depositor

    Shohei Mitani

    Article

    Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

    Insert

    channel rhodopsin-2 with H134R mutation
    

    Use

    Tags

    Expression

    Worm

    Mutation

    H134R

    Promoter

    inx-1

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-iRFP713-ORP9-PH

    Plasmid

    #214269

    Purpose

    Mammalian expression of near-IR fluorescent PI4P reporter iRFP713-ORP9-PH

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    iRFP713-ORP9(1-111) (Osbpl9 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSUB7

    Plasmid

    #169698

    Purpose

    Template plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C3 luc (T1 del)

    Plasmid

    #11358

    Depositor

    Donald McDonnell

    Article

    Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

    Insert

    C3 promoter (C3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PLG-bio-His

    Plasmid

    #53427

    Purpose

    Expresses full-length Plasminogen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PLG (PLG Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSU315

    Plasmid

    #169697

    Purpose

    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
    

    Use

    Tags

    1xHA tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry-FKBP-PI4KAC1001

    Plasmid

    #139311

    Purpose

    Recruitable PI4KA catalytic fragment

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G1986D

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5-FLAG-C7orf59

    Plasmid

    #42332

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

    Insert

    C7orf59 (LAMTOR4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SQT1659

    Plasmid

    #78743

    Purpose

    Human expression plasmid for AsCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXR001-mCD4: EF1a-RfxCas13d-P2A-mCD4-T2A-EGFP

    Plasmid

    #228359

    Purpose

    RfxCas13d (NLS-RfxCas13d-NLS) with 2A-EGFP and 2A-mCD4 for RNA knockdown

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Insert

    RfxCas13d
    

    Use

    Lentiviral

    Tags

    2A-EGFP, 2A-mCD4, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pARF6(Q67L)-CFP

    Plasmid

    #11387

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 6 (ARF6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-OSF-VPS15

    Plasmid

    #99326

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    VPS15 (PIK3R4 Human)
    

    Use

    Tags

    Strep-Strep-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

22. pLenti-CMV-eGFP-SV40p-BlasR

    Plasmid

    #228271

    Purpose

    pLenti-CMV-eGFP-SV40p-BlasR plasmid for ectopic expression of GFP

    Depositor

    Judy Lieberman

    Article

    Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-mTagBFP2-PLCδ-PH

    Plasmid

    #214275

    Purpose

    Mammalian expression of blue fluorescent PI(4,5)P2 reporter mTagBFP2-PLCδ-PH

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    mTagBFP2-PLCδ(1-175) (PLCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBa.GFP-myosin Vb tail 1436-1846

    Plasmid

    #162714

    Purpose

    Mammalian expression of rat WT Myosin Vb with deletion of the 1435 N-terminal residues

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Myosin Vb (Myo5b Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 1435 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA-Dest47- C11orf83-GFP

    Plasmid

    #65844

    Purpose

    Mammalian expression of C terminally GFP-tagged C11orf83/UQCC3

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    UQCC3 WT (UQCC3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Myo1E-pEGFP

    Plasmid

    #124658

    Purpose

    Mouse myosin-1e fused with EGFP

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    Myo1e (Myo1e Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. R26-H2B-EGFP HR donor vector

    Plasmid

    #137925

    Purpose

    R0SA26 knock-in vector with homologous recombination-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-H2B-EGFP-bpA with homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BII-TOP-tdT

    Plasmid

    #133395

    Purpose

    Piggybac vector for canonical Wnt signaling reporter

    Depositor

    Blair Madison

    Article

    Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

    Insert

    tdTomato-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    SuperTOPFlash

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAM233

    Plasmid

    #226655

    Purpose

    pcDNA3.1-p97-FLAG

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTJV1Sc-rpoB

    Plasmid

    #166978

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Inserts

    Ec86 Retron (ECD_00831 )
    lambda bet
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101 and VanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMT950

    Plasmid

    #228290

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDOE11 Lat52:mCherry transient

    Plasmid

    #124249

    Purpose

    Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.

    Depositor

    Attila Fehér

    Article

    Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TR-Csy4-R115A/R119A

    Plasmid

    #80696

    Purpose

    Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    Csy4-R115A/R119A
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Arg115 and Arg119 both mutated to Ala

    Promoter

    CBA

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-TK1

    Plasmid

    #100544

    Purpose

    mammalian expression of thymidine kinase 1

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

    Insert

    thymidine kinase 1 (TK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3 Flag TSC2 (S939A)

    Plasmid

    #14132

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 S939A (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A.

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

36. pBa.GFP-AP1/gamma1

    Plasmid

    #218801

    Purpose

    Mammalian expression of mouse Ap1 g1

    Depositor

    Marvin Bentley

    Article

    Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

    Insert

    Ap1 g1 (Ap1g1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKD4-Nter-APEX2

    Plasmid

    #112868

    Purpose

    Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

    Depositor

    Eric Cascales

    Article

    Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

    Insert

    APEX2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJS646

    Plasmid

    #183175

    Purpose

    Y2H vector compatible with pJS647 PYR1 insert

    Depositor

    Timothy Whitehead

    Article

    Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJMP1354

    Plasmid

    #119275

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-ALK7 T194D

    Plasmid

    #80886

    Purpose

    mammalian expression of ALK7 T194D

    Depositor

    Aristidis Moustakas

    Article

    Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

    Insert

    ALK7 (Acvr1c Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T194D (constitutively active)

    Promoter

    CMV

    Available since

    Aug. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

41. pcDNA3.1-SARS-CoV-2-E

    Plasmid

    #215814

    Purpose

    Expresses SARS-CoV-2 Envelope protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Envelope (E )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1-SARS-CoV-2-M

    Plasmid

    #215813

    Purpose

    Expresses SARS-CoV-2 Membrane protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Membrane (M )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA3.1-SARS-CoV-2-S (D614G)

    Plasmid

    #215838

    Purpose

    Expresses SARS-CoV-2 Spike protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGTRm

    Plasmid

    #127197

    Purpose

    pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA PCR template
    

    Use

    Template plasmid for generation of golden gate pc…

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pJRH623

    Plasmid

    #171633

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-non-targeting
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAS

    Plasmid

    #140008

    Purpose

    Plasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    4xPylT (Mma) cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    U25C

    Promoter

    h7SK

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCMV-mTagBFP2-ORP9-PH

    Plasmid

    #214267

    Purpose

    Mammalian expression of blue fluorescent PI4P reporter mTagBFP2-ORP9-PH

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    mTagBFP2-ORP9(1-111) (Osbpl9 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV-dSa-VP64-p65(100-261)-RTA(125-190)

    Plasmid

    #99679

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64-p65(101-261)-RTA(125-190)

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCAT007

    Plasmid

    #171634

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. Emi LC

    Plasmid

    #113666

    Purpose

    For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with anti-FIX(a) and anti-FX(a) Emi HC's.

    Depositor

    Robert Peters

    Article

    Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1

    Insert

    Emicizumab light chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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