159,134 results
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Plasmid#207788PurposeDonor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)DepositorInsertLAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS-Raichu-RhoA-CR
Plasmid#40258DepositorInsertRaichu-RhoA-CR (RHOA Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Eomes-3xT7
Plasmid#200888PurposeInducible lentiviral expression of EomesDepositorInsertEomes (Eomes Mouse)
UseLentiviral; Doxycycline inducibleTags3xT7ExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Venus
Plasmid#96940PurposeRetroviral plasmid containing Venus for labeling of cellsDepositorInsertVenus Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ninein N-ter
Plasmid#73520PurposeEncodes the N-terminus of mouse ninein (residues 1-373) with an EGFP tag at its N-terminusDepositorInsertninein N-ter (residues 1-373) (Nin Mouse)
UseTagsEGFP tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos16)GFP
Plasmid#89249PurposeExpresses GFP with +16 theoretical charge in E.ColiDepositorInsertHis6-(pos16)-GFP
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lsh_C2c2_WT
Plasmid#83487PurposeBacterial expression of WT L. shahii C2c2 CRISPR effectorDepositorInsertLsh_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one
Plasmid#183695PurposeExpresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selectiveDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-2A-MCS2
Plasmid#71782PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleavā¦TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Mm.cargo(Cas9)
Plasmid#174865PurposeExpresses mouse Peg10 cargoRNA encoding SpCas9DepositorInsertSpCas9 flanked by MmPeg10 UTRs
UseTagsHA-SV40 NLS and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-Mango II x 24
Plasmid#127587PurposeExpresses the mCherry-Mango II x 24 array from a TetON responsive promoterDepositorInsertmCherry-Mango II x24
UseTagsmCherryExpressionMammalianMutationPromoterpTRE Tet ONAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MbPylRS(IPYE)
Plasmid#104072PurposeExpression plasmid encoding MbPylRS(IPYE) and pylTDepositorInsertPlpp MbPylRS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, Aā¦PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58
Plasmid#171435PurposeExpression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cellsDepositorInsertLifeact-(GGS)X6-HaloTag C58
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
GNRHR-Tango
Plasmid#66297PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGNRHR (GNRHR Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYFP-ErbB2
Plasmid#66948PurposeMammalian expression of rat ErbB2 tagged with YFPDepositorInsertErbB2 (Erbb2 Rat)
UseTags6xHis, V5 Tag, and YFPExpressionMammalianMutation(See depositor comment below on mutations A24T anā¦PromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GCaMP6s
Plasmid#171156PurposeEncodes GCaMP6sDepositorInsertGCaMP6s
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.mCerulean.WPRE.RBG
Plasmid#105557PurposeAAV expression of mCerulean from CB7 promoterDepositorHas ServiceAAV1 and AAV9InsertmCerulean
UseAAVTagsExpressionMammalianMutationPromoterCB7Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tet1-dCas9
Plasmid#136650PurposeTet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertTet1 (Tet1 Mouse)
UseCRISPRTags3XFLag-NLS-Tet1-dCas9-NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PUC57_BAG3wt-eGFP
Plasmid#98182PurposeCodes for full length, GFP tagged, human wildtype Bag3DepositorInsertBAG3wt-eGFP (BAG3 Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry
Plasmid#207668PurposeTo express fluorescently labeled MS2 coat binding protein (MCP)DepositorInsertMCP-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PIK4CA
Plasmid#20577DepositorInsertPIK4CA (PI4KA Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus
Plasmid#161775Purpose3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interestDepositorTypeEmpty backboneUseLentiviral; MulticistronicTagsExpressionMammalianMutationPromoterUbCAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Px
Plasmid#183170PurposeConstruct for transient expression of peroxisomes-targeted mCherry in plants.DepositorInsertmCherry-Px
UseTags10 aa peroxisome targeting signal 1 (IHHPRELSRL) ā¦ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-IRAK4
Plasmid#23749DepositorInsertIRAK4 (IRAK4 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc wt MBS1-4
Plasmid#16564DepositorInsertc-MYC binding sites (MYC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti 4.1 Ex miR200c-141
Plasmid#35534Purpose3rd generation lentiviral vectorDepositorUseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-Thy1.1 DEST
Plasmid#17442PurposeDestination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reactionDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
TFORF0172
Plasmid#142551PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertONECUT2 (ONECUT2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-KHK
Plasmid#23586DepositorInsertKHK (KHK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Pd-mChe
Plasmid#183172PurposeConstruct for transient expression of plasmodesmata-targeted mCherry in plants.DepositorInsertPd-mCherry
UseTagsAtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDINā¦ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF3383
Plasmid#144859PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFKBID (NFKBID Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJOG1368
Plasmid#167301PurposeLevel 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-FluorocytosineDepositorInsertFCY-UPP fusion gene
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
RelA cFlag pcDNA3
Plasmid#20012DepositorInsertRelA (Rela Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVG
Plasmid#64084PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein geneDepositorInsertVSV glycoprotein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBinit
Plasmid#110100PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTagDepositorTypeEmpty backboneUseTagsmyc, HisExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF0662
Plasmid#143063PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCHD6 (CHD6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1
Plasmid#58433PurposeInduces Citrine expression from a promoter containing 2 lexA boxes in yeast cellsDepositorInsertCitrineA206K
UseTagsExpressionYeastMutationPromoterinsul-(lexA-box)2-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SENP8
Plasmid#18066DepositorInsertSENP8 (SENP8 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH-HHT2
Plasmid#86482Purposeyeast expression of HHT2 with His10-TEV-HA tagDepositorInsertHHT2 (HHT2 Budding Yeast)
UseTagsHIS10-TEV-HA (HTH)ExpressionYeastMutationPromoterGAL1Available sinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19/mChe-Cs
Plasmid#183171PurposeConstruct for transient expression of cytoskeleton-targeted mCherry in plants.DepositorInsertmCherry-Cs
UseTags363 aa of A. thaliana FIMBRIN 1 (AtFIM1)ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJOG1336
Plasmid#167303PurposeLevel 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)DepositorInsert3'UTR and Terminator SlOLE2
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOG1334
Plasmid#167302PurposeLevel 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)DepositorInsertPromoter SlOLE2
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-10Myc
Plasmid#181939PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xMyc
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro-IL8
Plasmid#189608PurposeRetroviral expression vector for IL8DepositorInsertIL8 (CXCL8 Human)
UseRetroviralTagsExpressionMutationPromoterSV40 early promoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Golgi
Plasmid#183169PurposeConstruct for transient expression of Golgi apparatus-targeted mCherry in plants.DepositorInsertmCherry-Golgi
UseTagsAtRER1B, A. thaliana endoplasmic reticulum retrieā¦ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
ARHGEF2A-c055
Plasmid#205103PurposeARHGEF2 protein expressionDepositorInsertARHGEF2 (ARHGEF2 Human)
UseTags6His-TEVExpressionBacterialMutationWTPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
ARHGEF2A-c052
Plasmid#205102PurposeARHGEF2 protein expressionDepositorInsertARHGEF2 (ARHGEF2 Human)
UseTags6His-TEVExpressionBacterialMutationWTPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
RHOAA-c035
Plasmid#205118PurposeRHOA protein expressionDepositorInsertRHOA (RHOA Human)
UseTags6His-TEV and AviTagExpressionBacterialMutationWTPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18c
Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
UseTags6xHis tag and TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only