We narrowed to 6,120 results for: nls
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Plasmid#37334DepositorInsertNLS CFP
UseTagsExpressionMutationPromoterCMVAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
NLS-LgBiT-FRB
Plasmid#183403PurposeExpresses nuclear-localized LgBiT-FRBDepositorInsertLgBiT-FRB
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee-NLS
Plasmid#140900Purposemammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertSpiCee-NLS
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRY2FL(deltaNLS)-mCherryN1
Plasmid#26871PurposeMammalian expression of cytoplasmic CRY2-mCherryDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationNLS sequences deletedPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-EKARrEV-NLS
Plasmid#173855PurposeEncoding EKARrEV-NLS.DepositorInsertEKARrEV-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
4xNLS-pMJ915v2-sfGFP
Plasmid#88921PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
mApple-PCNA-19-NLS-4
Plasmid#54937PurposeLocalization: Nucleus, Excitation: 568, Emission: 592DepositorInsertPCNA-19 NLS (PCNA Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.HA_mCherry-NLS
Plasmid#178209PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.HA and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-3XNLS
Plasmid#209392PurposeTransiently expressing TurboID fused with 3X nuclear localization signal (NLS) in plantaDepositorInsert3XHA-TurboID-3XNLS
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLS-LEXY (pDN138)
Plasmid#72657Purposegolden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)DepositorTypeEmpty backboneUseGolden gate entry vectorTagsNLS and mCherry-LEXYExpressionMammalianMutationPromoterCMV-FAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-His:CRY2:nls:VPR
Plasmid#135989PurposeExpresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cellsDepositorInsertsCRY2 domain fused to the VPR activation domain
CRY2 domain fused to the VPR activation domain
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQC NLS mCherry IX
Plasmid#37354DepositorInsertNLS-mCherry
UseTagsExpressionMutationPromoterAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-enCas12a-2xNLS
Plasmid#209021PurposeLentiviral vector expressing human codon-optimized enCas12a with 2xNLSDepositorInsertenCas12a-2xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationE174R/S542R/K548RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUGW NLS-Cre
Plasmid#183425PurposeLentiviral Cre vectorDepositorInsertNLS-Cre
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-BiNLS-fYap
Plasmid#176623Purposeexpresses optofYap in zebrafishDepositorInsertYes associated protein (yap1 Zebrafish)
UseZebrafish expressionTagsmCherryExpressionMutationPromoterSP6Available sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN017-SpCas9-hiNLS-t-M4
Plasmid#234455PurposeExpresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-TagRFP-NLS
Plasmid#184446PurposeNucleus-targeted TagRFP expression in mammalian cellsDepositorInsertTagRFP-3xNLS
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis-parallel1-NLSH2BCas9
Plasmid#112065PurposeExpression and purification of NLSH2BCas9 for fungal gene editingDepositorInsertNLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
UseCRISPRTagsHis tagExpressionBacterialMutationCas9 was codon optimized for Aspergillus nigerPromoterpromoter in vectorAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_CAG_3xTurboGFP-NLS
Plasmid#229777PurposeBxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)DepositorInsert3x TurboGFP
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
plenti-Lb-Cas12a-2xNLS
Plasmid#155046PurposeLentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoterDepositorInsertLbCas12a
UseCRISPR and LentiviralTagsNucleoplasmin NLS, Myc-tag and SV40 NLSExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIB1(deltaNLS)-pmGFP
Plasmid#28240PurposeExpresses CIB1 fused to plasma membrane-localized EGFPDepositorInsertCIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)
UseTagsEGFPExpressionMammalianMutationNLS sequences mutatedPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pICE-NLS-mCherry
Plasmid#60364PurposePlasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertNLS-mCherry
UseTagsNLS from SV40 T antigen and mCherryExpressionMutationPromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-2C-NLS in pCSDest
Plasmid#126638PurposeExpresses LbCas12a-2C-NLS in mammalian cellDepositorInsertLbCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
459 p3E-2A-nlsEGFPpA
Plasmid#195969PurposeGateway p3E 2A fluorophoreDepositorInsert2A-nlsEGFP (nuclear)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCF140_Gag-SpyCas9-NLS
Plasmid#225959PurposeCMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.DepositorInsertCMV-Intron-Gag-SpyCas9-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-trCIB1
Plasmid#64119PurposePhotoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1DepositorInsertdCas9-trCIB1 fusion
UseTagsHA and NLSExpressionMammalianMutationdCas9 (D10A H840A, catalytically inactive), trCIB…PromoterCMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHeACT-mEGFP-NLS
Plasmid#205003PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAd-PV-NLS-DsRed
Plasmid#17287DepositorInsertPV-DsRed-NLS-CD (Pvalb Rat)
UseAdenoviralTagsMycExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-EKARrEV-NLS
Plasmid#173854PurposeA lentiviral vector for EKARrEV-NLSDepositorInsertEKARrEV-NLS
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
spCas9-NLS-MAV
Plasmid#124212PurposeSpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)DepositorInsertcas9
UseCRISPRTagsHis6, MBP, TEV Cleavage site, and two copies of S…ExpressionBacterialMutationPromoterT7Available sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-MCP-3XBFPnls
Plasmid#75383PurposeMCP-3XBFPnlsDepositorInsertMCP
UseLentiviralTags3XBFPExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
NLS-4LNDD-pcDNA3.1(+)
Plasmid#172313PurposeExpresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain
UseTags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
NLSx3-CRY2PHR-p65
Plasmid#64124PurposePhotoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.DepositorInsertCRY2PHR-p65
UseTagsHis, NLSx3, and V5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-NLS-M-MLV-RT
Plasmid#181804PurposepET28a-NLS-M-MLV RTDepositorInsertNLS-M-MLV-RT
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-NLS-PTEN
Plasmid#206984PurposeLentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLSDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsNLSExpressionMammalianMutationPromotertet-on promoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUAS5x:GFPNLS-SV40pA
Plasmid#155122PurposeTol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUAS5x:GFPNLS
UseZebrafish expressionTagsExpressionMutationPromoterQUAS5x-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pN1-NLS-pHlu
Plasmid#129320PurposepH measurement, nucleusDepositorInsertpHluorin (from H. Imamura)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCS:GFPnls-T2a-ER-Cre-ER
Plasmid#200106PurposeVector for LFNGDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
GANES-DEVD-BNLS
Plasmid#50842PurposeExpresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.DepositorInsertddGFP A and ddGFP B
UseTagsNES sequence LALKLAGLDIGS placed after ddGFP A an…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Prab-3::NLS::GCaMP6s
Plasmid#68119PurposeNuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. ElegansDepositorInsertrab-3::NLS::GCaMP6s
UseTagsExpressionWormMutationPromoterrab-3Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pN1-NLS-QUE37C
Plasmid#129323PurposeATP measurement, nucleusDepositorInsertQUEEN-37C
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
epB_CAG_TetRFlag-nls-tdTom_DEx4
Plasmid#119909PurposeePiggyBac mammalian expression plasmid for TetR-tdTom fusionDepositorInsertTetRFlag-nls-tdTom
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbEBFP2-NLS
Plasmid#176682PurposeExpression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)DepositorInsertEBFP2
UseTagsExpressionInsectMutationPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR-NLS
Plasmid#64931PurposeNuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.DepositorInsertTORCAR-NLS (EIF4EBP1 Human, Synthetic)
UseTagsCerulean, Nuclear localization signal (NLS), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-3xnls-Tq-Ca-FLITS
Plasmid#145030PurposepiggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-Tq-Ca-FLITS
UseTags3xnls-Tq-Ca-FLITSExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRvUBC-mEGFP-NLS
Plasmid#205010PurposeThe vector contains 1kbp of the upstream and downstream regions of the ubiquitin c gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ubiquitin c gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc:P4:gs:nls:ABI
Plasmid#135985PurposeExpresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cellsDepositorInsertP4 coiled-coil fused to the ABI domain
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLS-LucWT-GFP
Plasmid#127709PurposeThe expressed ORF encodes nucleus location signal with GFP and WT Luciferase.DepositorInsertLuciferase
UseTagsEGFP and FLAG-NLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQC NLS TdTomato IX
Plasmid#37347DepositorInsertNLS Tdtomato
UseTagsExpressionMutationPromoterAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-PCNA-19-SV40NLS-4
Plasmid#55250PurposeLocalization: Nucleus, Excitation: 383, Emission: 448DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTags3xSV40 NLS and EBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits