178,225 results
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Plasmid#16623DepositorTypeEmpty backboneExpressionMammalianAvailable SinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only
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pLPC hRap1-FL
Plasmid#12542DepositorInsertRepressor Activator Protein 1 (TERF2IP Human)
UseRetroviralAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRetroSuper-GFP Smad3
Plasmid#15723DepositorAvailable SinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRetroSuper-GFP Smad4
Plasmid#15724DepositorAvailable SinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-SUMO1
Plasmid#13989DepositorAvailable SinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk3a2
Plasmid#13759DepositorAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-14-3-3 zeta GST
Plasmid#13278DepositorAvailable SinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro CXCR4 siRNA-2
Plasmid#12272DepositorAvailable SinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK1037
Plasmid#8833DepositorInsertTVMV protease, catalytic domain
ExpressionBacterialAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
p4027 GST-ubiquitin
Plasmid#10861DepositorAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pARF1(T31N)-CFP
Plasmid#11384DepositorInsertADP Ribosylation Factor 1 (ARF1 Human)
TagsCFPExpressionMammalianMutationChanged Threonine 31 to Asparagine.Available SinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBMN-Z-IN
Plasmid#1735PurposeThis retroviral vector expresses lacZ and has an IRES-NEO downstreamDepositorInsertLacZ-IRES-Neo
UseRetroviralAvailable SinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCruzHA SIRT1 G261A
Plasmid#10963DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
401 pSG5L
Plasmid#10737DepositorTypeEmpty backboneExpressionMammalianAvailable SinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3
Plasmid#209723PurposeExpresses mitochondrially targeted low affinity ratiometric ATP sensorDepositorInsert(mito).iATPSnFR2.A95A.A119L.miRFP670nano3
UseAAVExpressionMammalianPromoterCAGAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AYBEv3
Plasmid#193967PurposeAn engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertecTadA8e-nSpCas9(D10A)-MPG(v3)
ExpressionMammalianMutationG163R, N169S, S198A, K202A, G203A, S206A, K210APromoterhU6, EF1a, CBhAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmIgG2a-Tn5
Plasmid#184288PurposeAnti-mouse IgG2a Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG2a-Tn5
Available SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6f
Plasmid#182548PurposeExpresses GCaMP6f on the cytosolic side of the ER (P-450site)DepositorInsertER localized P450
ExpressionBacterial and MammalianAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-rM3D(Gs)-mCherry (AAV8)
Viral Prep#50458-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-DIO-rM3D(Gs)-mCherry (#50458). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-rM3D(Gs)-mCherry plasmid DNA. hSyn-driven, Cre-dependent rM3D(Gs) receptor with an mCherry reporter for chemogenetic excitation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO mScarlet (AAV1)
Viral Prep#131002-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-Ef1a-DIO mScarlet (#131002). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO mScarlet plasmid DNA. EF1a-driven, Cre-dependent mScarlet expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmScarlet (Cre-dependent)Available SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon DREADD Gi-mCherry
Plasmid#177672PurposeExpresses DREADD hM4D (Gi) in Cre and Flp expressing cellsDepositorHas ServiceAAV8InsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianPromoternEFAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
spNW25
Plasmid#173484PurposeExpresses spNW25 in Bl21DepositorInsertspNW25
TagsHistagExpressionBacterialAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB_iETV2_P2A_GFP_Puro
Plasmid#168805PurposePiggyBac transposon construct with doxycycline inducible ETV2 + GFP expressionDepositorInsertETV2 (ETV2 Human, Synthetic)
UseSynthetic Biology; PiggybacExpressionMammalianPromoterTREAvailable SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gs(short)-CASE
Plasmid#168124PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).DepositorUseLuciferaseTagscpVenus on GNG1ExpressionMammalianMutationNLuc is inserted at N136/V137 within GNASAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-sRGECO (AAV8)
Viral Prep#137128-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-sRGECO (#137128). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-sRGECO plasmid DNA. EF1a-driven, Flp-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a his6-GST-WTPhi29DNAP
Plasmid#166863PurposeTo produce a dual affinity-tagged Phi29 DNA polymeraseDepositorInsertPhi29 Polymerase codon-optimized for overexpression in E. coli
TagsGST and his6ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam C term 3C eGFP StrepII
Plasmid#160686PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTags3C eGFP StrepIIExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
ExpressionBacterialPromoterJ23119Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIE1
Plasmid#136355PurposeSelection by Inducible EffectorsDepositorInsertsType VI effector bfe1
Type VI effector bte1
BT_4676
tetR
UseUnspecifiedMutationATG removed to add signal sequence before genePromoterP1TDP-GHO23Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-CAG-SARS-CoV2-Spike D614G-HA
Plasmid#158761Purpose3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M PaoDepositorInsertSARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
UseLentiviralTagsHAExpressionMammalianMutationSpike D614GPromoterCAGAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA
Plasmid#154868PurposeHuman synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherryDepositorHas ServiceAAV Retrograde and AAV8InserthM3D(Gq)-mCherry
UseAAV; Flp-frtTagsmCherryExpressionMammalianPromoterHuman Synapsin-1Available SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_Ado1.0
Plasmid#153285PurposeExpress the adenosine sensor GRAB_Ado1.0 in neuronsDepositorHas ServiceAAV1InsertAdenosine sensor GRAB_Ado1.0
UseAAVPromoterhSynAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiV2-dCas9-VP64
Plasmid#141104PurposeExpression of gRNA and dCas9-VP64 from the same plasmid for CRISPRaDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV R-CEPIA4mt
Plasmid#140463PurposeRed fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorInsertR-CEPIA4mt
TagsMycExpressionMammalianAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-SV40LT
Plasmid#136616PurposeDox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cellsDepositorInsertSV40 large T antigen
UseLentiviralExpressionBacterialAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-All-in-one-hCascade
Plasmid#134919PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.DepositorInsertCascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
ExpressionMammalianPromoterCAGAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max-NG
Plasmid#138159PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10A + …PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-MYC-IRES-lucOS
Plasmid#129776PurposeTransposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339DepositorInserthuman MYC (MYC Human)
TagsOS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…ExpressionMammalianPromoterEF1AAvailable SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-WT
Plasmid#133753PurposeWild type human TDP-43 in pLVX-Puro lentiviral vectorDepositorAvailable SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmNeonGreenHO-G
Plasmid#127912Purposea mammalian expression backbone vector for humanized mNeonGreenDepositorInserthumanized mNeonGreen
TagsmNeonGreenExpressionMammalianAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
CDH5-AbR-GFP
Plasmid#122970PurposeLenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.DepositorInsertsCDH5p
CopGFP
Zeocin
UseLentiviralExpressionMammalianMutationNAAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5
Plasmid#124362PurposeOverexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)DepositorInsertTornado-F30-Broccoli-aptNFkB#5
ExpressionMammalianPromoterU6+27Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EZH2
Plasmid#116738PurposeLentiviral expression of EZH2DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-A0201-SABR-Backbone
Plasmid#119050Purpose3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterMNDAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetON-Sox10
Plasmid#115242PurposeLentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)DepositorInsertSOX10 (SOX10 Human)
UseLentiviralAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
GATA3-pCDNA3.1
Plasmid#115271PurposeExpresses human GATA3 in mammalian cellsDepositorAvailable SinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZS165 CRISPEY HH-HDV NotI
Plasmid#114451PurposeYeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozymeDepositorTypeEmpty backboneUseCRISPR; Cen/arsExpressionYeastPromoterScGAL7pAvailable SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI
Plasmid#115267PurposeExpresses LbuCas13a from a SUMO-tagged bacterial expression constructDepositorInsertLbuCas13a
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-dLight1.2
Plasmid#111068PurposeTo generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoterDepositorHas ServiceAAV1 and AAV5InsertdLight1.2
UseAAVTagsFlag tagAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PTE2A
Plasmid#111773PurposeQuadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2ADepositorTypeEmpty backboneUseCloning intermediatePromoterT7Available SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only