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176,141 results

Showing: 8401 - 8450 of 176141 results

  1. pGL4.22-VEGF-HRE::dLUC

    Plasmid
    #128096
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Promoter
    inserted VEGF hypoxia response elements (HRE) (x5)
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128096-plasmid-map-sequence-id-249669

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  3. RNA-Protein Landing Pad

    Plasmid
    #226162
    Purpose
    Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression
    Depositor
    Dustin Maly
    Article
    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.
    Inserts
    mTagBFP2-P2A-iCasp9
    Reverse tetracycline transactivator 3
    Hygromycin-IRES-EGFP
    Use
    Synthetic Biology
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    226162-plasmid-map-sequence-id-450826

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  5. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  10. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  25. pSL21-VEX

    Plasmid
    #158230
    Purpose
    Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)
    Depositor
    Junwei Shi
    Article
    Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Expression
    Mammalian
    Promoter
    U6 promoter for crRNA expression and EFS promoter…
    Available Since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158230-plasmid-map-sequence-id-317503

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  28. pNX24-RFP-MS2SL

    Plasmid
    #227819
    Purpose
    Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene
    Depositor
    Jeffrey Gerst
    Article
    Haimovich et al bioRxiv 2024.11.06.622258
    Inserts
    3xHA-RFP-24xMS2SL
    Neo resistance
    Expression
    Mammalian
    Promoter
    CMV and SV40
    Available Since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    227819-plasmid-map-sequence-id-469648

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  30. pUC57-mitoTPNOX

    Plasmid
    #87854
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    mitoTPNOX
    Tags
    C-terminal FLAG tag with a linker and mitochondri…
    Expression
    Bacterial
    Available Since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    87854-plasmid-map-sequence-id-175127

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  32. DT480

    Plasmid
    #80413
    Purpose
    Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Article
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Insert
    human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
    Expression
    Mammalian
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80413-plasmid-map-sequence-id-151399

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  42. pcDNA3-TLR2-YFP

    Plasmid
    #13016
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 2 (TLR2 Human)
    Tags
    YFP
    Expression
    Mammalian
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13016-plasmid-map-sequence-id-472886

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  44. pdCas9

    Plasmid
    #46569
    Purpose
    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9
    CRISPR array
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46569-plasmid-map-sequence-id-364492

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  49. pCS2+-PIF6-EGFP

    Plasmid
    #154913
    Purpose
    1-100 amino acids of Arabidopsis PIF6 (Gene ID: 825382), tagged with EGFP fluorescent protein. Mammalian codon optimised.
    Depositor
    Clare Buckley, Jonathan Clarke
    Article
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Insert
    phytochrome interacting factor 3-like 2 (PIL2 Mustard Weed)
    Use
    Xenopus/avian/zebrafish
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    1-100 amino acids of Arabidopsis PIF6
    Promoter
    CMV
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154913-plasmid-map-sequence-id-308719

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  50. pPBC-LG6f

    Plasmid
    #62808
    Purpose
    pPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP6f-ITR
    Expression
    Bacterial and Mammalian
    Promoter
    CAG
    Available Since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    62808-plasmid-map-sequence-id-123121

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Showing: 8401 - 8450 of 176141 results