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179,538 results

Showing: 8401 - 8450 of 179538 results

  1. pAAV-CamKII-ArchT-GFP (PV2527) (AAV Retrograde)

    Viral prep
    #99039-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CamKII-ArchT-GFP (PV2527) (#99039). In addition to the viral particles, you will also receive purified pAAV-CamKII-ArchT-GFP (PV2527) plasmid DNA. CaMKII-driven ArchT-GFP expression for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Publication
    Han et al Front Syst Neurosci. 2011;5:18. Epub 201
    Promoter
    CaMKII
    Tags
    GFP
    Available since
    May 15, 2019
    Availability
    Academic Institutions and Nonprofits only

  3. Halo-Sec61-C-18

    Plasmid
    #123285
    Purpose
    Localization: Endoplasmic Reticulum
    Depositor
    Kevin McGowan
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sec61 (SEC61B Human)
    Tags
    HaloTag7
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    123285-plasmid-map-sequence-id-242690

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  6. pGEX6P1-DEST

    Plasmid
    #119749
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119749-plasmid-map-sequence-id-233993

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  7. pCT5-bac1.8

    Plasmid
    #119871
    Purpose
    Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli
    Depositor
    Claudia Schmidt-dannert
    Publication
    Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p
    Inserts
    CymR'
    sfGFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    SNP in 592 bp
    Promoter
    PserA promoter and Pveg promoter with CuO sequence
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119871-plasmid-map-sequence-id-232788

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  9. pLHCX-ATF4 mScarlet NLS

    Plasmid
    #115970
    Purpose
    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)
    Depositor
    David Andrews
    Publication
    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:
    Insert
    activating transcription factor 4 (ATF4 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA tag, c-myc NLS, and mScarlet-I
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    115970-plasmid-map-sequence-id-222082

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  11. CRISPi Kit

    Plasmid kit
    #1000000136
    Purpose
    Plasmids for performing CRISPR/Cas9 mediated gene and genome editing in Pichia pastoris.
    Depositor
    Brigitte Gasser
    Application
    Genome Editing
    Vector type
    Yeast Expression
    Editing type
    CRISPR
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE

    Plasmid
    #104492
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has service
    AAV1, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    jGCaMP7f
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    36 Articles
    104492-plasmid-map-sequence-id-381181

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  14. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

    Plasmid
    #107270
    Purpose
    Expresses Cas9 upon induction with doxicycline.
    Depositor
    Pablo Menéndez
    Publication
    Castano et al Stem Cell Res. 2017 May;21:137-140. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    hSpCas9
    rtTA
    bGH polyA-SV40polyA
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and TRE
    Available since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    107270-plasmid-map-sequence-id-260738

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  18. AID* Kit

    Plasmid kit
    #1000000120
    Purpose
    Plasmid for fusion of a protein of interest to the auxin-inducible degron to control protein levels in vivo.
    Depositor
    Helle Ulrich
    Application
    Gene Expression and Labeling
    Vector type
    Yeast Expression
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  24. pcDNA4 HisMax-V5-GFP-RRBP1

    Plasmid
    #92150
    Purpose
    Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.
    Depositor
    Alice Ting
    Publication
    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RRBP1 (RRBP1 Human)
    Tags
    Calreticulin signal peptide, EGFP (A207K mutation…
    Expression
    Mammalian
    Mutation
    In RRBP1: N951S and T1329L. E1279 is also missing.
    Promoter
    CMV
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    92150-plasmid-map-sequence-id-181297

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  25. AbVec2.0-IGHG4

    Plasmid
    #99578
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG4 isotype
    Depositor
    Hedda Wardemann
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    hCMV IE1 gene promoter
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    99578-plasmid-map-sequence-id-192595

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  26. pCAG dCas9-KRAB-2A-EGFP

    Plasmid
    #92396
    Purpose
    CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9_KRAB_2A_EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    92396-plasmid-map-sequence-id-178234

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  27. pCMV-DeAct-SpvB

    Plasmid
    #89446
    Purpose
    Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells
    Depositor
    Brad Zuchero
    Publication
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    CMV
    Available since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    89446-plasmid-map-sequence-id-177125

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  30. PB53x EF1-Dendra2-RPB1Amr

    Plasmid
    #81228
    Purpose
    expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system
    Depositor
    Timothée Lionnet
    Publication
    Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1
    Insert
    Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    N792D alpha amanitin resistance mutation (Bartolo…
    Promoter
    EF1a
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    81228-plasmid-map-sequence-id-394051

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  31. MP9956:SFG.GD3synthase-2A-GD2synthase

    Plasmid
    #75013
    Purpose
    Retroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.
    Depositor
    Martin Pule
    Publication
    Thomas et al PLoS One. 2016 Mar 31;11(3):e0152196. do
    Inserts
    murine GD3 synthase
    murine GD2 synthase
    Use
    Retroviral; Momlv retroviral vector
    Expression
    Mammalian
    Available since
    Sept. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    75013-plasmid-map-sequence-id-141876

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  32. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Publication
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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  34. pKA-207I10

    Plasmid
    #79845
    Purpose
    Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter
    Depositor
    Vladislav Verkhusha
    Publication
    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
    Expression
    Mammalian
    Mutation
    RpPpsR2 cysteine 439 changed to serine
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    79845-plasmid-map-sequence-id-149588

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  40. SapTrap Kit

    Plasmid kit
    #1000000077
    Purpose
    A modular toolkit for building CRISPR targeting vectors to insert genetic tags in the C. elegans genome.
    Depositor
    Erik Jorgensen
    Application
    Genome Editing
    Vector type
    Worm Expression
    Editing type
    CRISPR
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  41. pCDH-EF1-Luc2-P2A-copGFP

    Plasmid
    #72485
    Purpose
    Express Luc2 and copGFP
    Depositor
    Kazuhiro Oka
    Insert
    Luc2-P2A-copGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    6 Articles
    72485-plasmid-map-sequence-id-132335

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  46. pBa-MARK2-eGFP

    Plasmid
    #66706
    Purpose
    mammalian expression vector for MARK2 with a C-terminal eGFP
    Depositor
    Gary Banker, Marvin Bentley
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MARK2 (MARK2 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    chicken beta actin
    Available since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    66706-plasmid-map-sequence-id-114008

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Showing: 8401 - 8450 of 179538 results