178,225 results

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pHR SFFVp YFP-PIF-SOS2cat

Plasmid

#50851

Purpose

Optogenetic Ras activation system; cytoplasmic component

Depositor

Wendell Lim

Article

Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

Inserts

SOS2cat (SOS2 Human)
PIF6 1-100

Use

Lentiviral

Tags

YFP

Mutation

residues 566–1049

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt1

Plasmid

#49186

Purpose

cDNA of Akt1 with myristyolation signal in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pLB1080

Plasmid

#37826

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

ATP7B (ATP7B Human)

Tags

mEGFP

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pRK6-HA-TAK1

Plasmid

#44160

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only
pcDNAFLAG-ATM-kd

Plasmid

#43908

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

ataxia telangiectasia mutated (ATM Human)

Tags

FLAG and His

Expression

Mammalian

Mutation

kinase dead (D2870A and N2875K)

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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HA Seh1L pRK5

Plasmid

#46331

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Seh1 (SEH1L Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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Csy4H29AS50C

Plasmid

#45029

Depositor

Jennifer Doudna

Article

Lee et al Proc Natl Acad Sci U S A. 2013 Apr 2;110

Insert

Csy4H29AS50C

Tags

His6-MBP

Expression

Bacterial

Mutation

H29A mutation to abolish catalytic activity and S…

Promoter

T7

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
Flag-NRF1

Plasmid

#34707

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pMJ824

Plasmid

#39314

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAD48-daf-2 RNAi

Plasmid

#34834

Depositor

Cynthia Kenyon

Article

Dillin et al Science. 2002 Oct 25;298(5594):830-4.

Insert

daf-2 (daf-2 Nematode)

Use

RNAi

Expression

Bacterial

Available Since

June 11, 2012

Availability

Academic Institutions and Nonprofits only
LZRS-Rfa

Plasmid

#31601

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Type

Empty backbone

Use

Retroviral

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
LeGO-T2

Plasmid

#27342

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, tdTomato

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pGCDNsam-Foxa1-IRES-GFP

Plasmid

#33007

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A1 (Foxa1 Mouse)

Use

Retroviral

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-TGF beta Type II receptor Flag

Plasmid

#31719

Depositor

Rik Derynck

Insert

TGF beta Type II Receptor (TGFBR2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only
GST-PRMT7

Plasmid

#34693

Depositor

Steven Clarke

Article

Miranda et al J Biol Chem. 2004 May 28;279(22):22902-7

Insert

PRMT7 (PRMT7 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3β-FLAG-CBP-LD-HA

Plasmid

#32906

Depositor

Tso-Pang Yao

Article

Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

Insert

C/EBP (Crebbp Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

L1435A/D1436A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC157-20XUAS-IVS-B3::PEST

Plasmid

#32136

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B3::PEST

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNAFlagMenin WT

Plasmid

#32079

Depositor

Matthew Meyerson

Article

Francis et al PLoS One. 2011 . 6(1):e16119.

Insert

Menin

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-MEKDD

Plasmid

#31202

Purpose

Gateway entry vector containing MEKDD.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

MAPKK1 (MAP2K1 Human)

Use

Gateway Cloning

Mutation

S218D S222D

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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pBF3038

Plasmid

#26850

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Insert

CreA recombinase

Use

Cre/Lox

Expression

Yeast

Mutation

N/A

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pZS*12-MS2-GFP

Plasmid

#26245

Depositor

Philippe Cluzel

Article

Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

Insert

MS2-GFP

Expression

Bacterial

Mutation

five amino acid linker (Ser, Gly, Gly, Gly, Gly) …

Available Since

Nov. 22, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pEiLOV-N1

Plasmid

#26588

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Expression

Mammalian

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 luciferase

Plasmid

#26612

Depositor

Jie Chen

Article

Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

Insert

firefly luciferase

Expression

Mammalian

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pMSCV-mLin28A

Plasmid

#26357

Depositor

George Daley

Article

Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

Insert

lin-28 homolog A (Lin28a Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pTomo-Ras

Plasmid

#26292

Depositor

Inder Verma

Article

Marumoto et al Nat Med. 2009 Jan . 15(1):110-6.

Insert

HRAS-V12 (HRAS Human)

Use

Lentiviral

Tags

Flag

Mutation

G12V

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-BMPR2

Plasmid

#23669

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BMPR2 (BMPR2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
GFP-RhoC

Plasmid

#23226

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoC (RHOC Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pCyPet-Rac1(WT)

Plasmid

#22782

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (wt) (RAC1 Human)

Tags

CyPet

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PLK1

Plasmid

#20589

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PLK1 (PLK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
mFGF2

Plasmid

#22084

Depositor

Gail Martin

Insert

FGF2 (Fgf2 Mouse)

Use

Bacterial

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pENTR/pSM2(U6) (w51-1)

Plasmid

#17387

Purpose

Gateway entry vector for miRNA expression under U6 promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
PKC beta II DN

Plasmid

#16385

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
WGA-D

Plasmid

#17989

Depositor

Natasha Raikhel

Article

Smith et al Plant Mol Biol. 1989 Nov . 13(5):601-3.

Insert

wheat germ agglutinin isolectin D

Expression

Bacterial

Mutation

N/A

Available Since

July 10, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3 CDK9 (D167N) HA (P#811)

Plasmid

#14641

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Tags

HA

Expression

Mammalian

Mutation

D167N. Kinase negative mutant. Please see deposit…

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
Keratin 18 (pcDNA3)

Plasmid

#18064

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBI-GFP-Bub1-wt

Plasmid

#16624

Depositor

Bert Vogelstein

Article

Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Insert

BUB1 (BUB1 Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5myc RhoA L63

Plasmid

#15900

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

RhoA (RHOA Human)

Tags

myc

Expression

Mammalian

Mutation

Q63L

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
p406TEF1

Plasmid

#15976

Depositor

Nicolas Buchler, Fred Cross

Insert

TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

TEF1 promoter is between SacI and XbaI restrictio…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2A

Plasmid

#15780

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2A (UBE2A Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX TIF1gamma

Plasmid

#15734

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma (TRIM33 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pBABE HAII

Plasmid

#14738

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

Type

Empty backbone

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pmKalama1-Nuc

Plasmid

#14894

Purpose

Mammalian expression of mKalama1 blue fluorescent protein with NLS

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

pmKalama1-Nuc

Tags

NLS

Expression

Mammalian

Mutation

I178T

Promoter

CMV

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pRK5 HA GST rap2a

Plasmid

#14952

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

rap2a (RAP2A Human)

Tags

HA-GST

Expression

Mammalian

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pQE9-His-p97(wt)

Plasmid

#14666

Depositor

Graham Warren

Article

Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Insert

p97 (Vcp Mouse)

Tags

RGS6xHis

Expression

Bacterial

Mutation

See depositor comments below.

Available Since

April 13, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pQLinkHD

Plasmid

#13668

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

Gateway cassette

Use

Co-expression

Tags

His

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
GEV 2

Plasmid

#12616

Depositor

John Louis

Article

Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Insert

GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)

Tags

G protein and His

Expression

Bacterial

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pCS-CG

Plasmid

#12154

Depositor

Inder Verma

Article

Miyoshi et al J Virol. 1998 Oct . 72(10):8150-7.

Insert

CMV-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSC-SP-PW-GFP (aka: pBOB-GFP)

Plasmid

#12337

Depositor

Inder Verma

Article

Marr et al J Mol Neurosci. 2004 . 22(1-2):5-11.

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ3H-Mst2 K56R

Plasmid

#12206

Depositor

Jonathan Chernoff

Article

Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

Insert

Mst2 (STK3 Human)

Tags

HA

Expression

Mammalian

Mutation

Catalytically inactive: K56R

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
3492 pcDNA3 Bad

Plasmid

#8778

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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