We narrowed to 18,717 results for: IRE
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Plasmid#172763PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-18
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCit-APPAP-gs
Plasmid#228090PurposeMammalian expression of five coiled coil segments in APPAP arrangment, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, APPAP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK-Cas9-gate
Plasmid#113743PurposeGateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.DepositorInsertCas9
UseCRISPR; Gateway destination vectorTagsExpressionMutationPromoterZ. Mays ubiquitin promoter, 35S promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsEDC4_S
Plasmid#147455PurposeMammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.DepositorInsertHsEDC4 (EDC4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL0915 (CRISPR with target 3)
Plasmid#130643PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertCRISPR(target-3)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-42
Plasmid#172758PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-42
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70
Plasmid#62897PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.DepositorInsertT2A-Gal4-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16-3xFLAG-LaG-2
Plasmid#172765PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)DepositorInsertLaG-16-3xFLAG-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5238
Plasmid#128711PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopG1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
CCR5_gRNA_pX459
Plasmid#192268PurposegRNA expression for CRISPR Genome Editing of CCR5DepositorInsertCCR5 guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1 FL
Plasmid#13469DepositorInsertMKP-1 FL (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70
Plasmid#62915PurposeCreating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-p65AD-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70
Plasmid#62903PurposeCreating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1DepositorInsertT2A-Gal4DBD-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
His-drebrin
Plasmid#40362DepositorInsertdrebrin E2 (DBN1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBS-KLF7-200-GFPfr1
Plasmid#169799PurposeTHe donor vector for the KLF7 gene locus.DepositorInsertDonor sequence to KLF7 neighbouring region for HR
UseHr donor vector for human actb gene.TagsExpressionMutationPartial sequence of GFP is inserted the sequence …PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-KLF7-1
Plasmid#169797PurposeExpresses Cas9 and guide RNA for the site neighbouring KLF7 gene.DepositorInsertguide RNA for KLF7 neighbouring region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER- Po Long P2A
Plasmid#164117PurposeDetect the long Peroxisomes-mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP and YFP Mt-ER- Po Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-L-Myc-L351P
Plasmid#50777Purposeretroviral expression of mouse L-Myc L351P mutantDepositorInsertL-Myc-L351P (Mycl Mouse)
UseRetroviralTagsExpressionMammalianMutationL351P (does not bind to Max)PromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cas9-PCV
Plasmid#123644PurposeExpresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coliDepositorInsertsUseTags6xHis and SUMOExpressionBacterialMutation2-116PromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-6
Plasmid#172741PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-6
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5052
Plasmid#128729PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAM1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5951
Plasmid#128719PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopR1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Stat1 alpha Y701F pRc/CMV
Plasmid#8701DepositorInsertSTAT1 alpha Y701F (STAT1 Human)
UseTagsExpressionMammalianMutationY701FPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMALcPP-MS2cp
Plasmid#203351PurposeExpresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteriaDepositorInsertMS2 coat protein
UseTagsHx6-MBPExpressionBacterialMutationV75E and A81G, to prevent capsid assemblyPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
NylB'-SCY_pET21b
Plasmid#215417PurposeExpression construct for NylB'-SCY gene, codon optimized for expression in E. coliDepositorInsertNylB'-SCY
UseTagsExpressionBacterialMutationR187S/F264C/D370YPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GST-beta-arrestin1
Plasmid#36918DepositorInsertbeta-arrestin1 (Arrb1 Rat)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMALB
Plasmid#161784PurposeGateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterBidirectional minhCMV and hPGKAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR V2 sgMTHFD1L_1
Plasmid#106316PurposeExpress Cas9 and sgRNA targeting MTHFD1LDepositorInsertsgRNA targeting MTHFD1L
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2 WC visual barcode
Plasmid#158669Purposetagging clonal populations with whole cell mTurquoise2 fluorescent proteinDepositorInsertmTurquoise2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70
Plasmid#62898PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2.DepositorInsertT2A-Gal4-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCPP5108
Plasmid#128726PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAA1-2
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5107
Plasmid#128725PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAA1-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-TetR-KRf-TetR
Plasmid#216270PurposeConstruct 3: TetR - Lipid binding domain KRφ - TetRDepositorInsertTetR - Lipid binding motif (KRf) - TetR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
drebrin-His
Plasmid#40363DepositorInsertdrebrin E2 (DBN1 Human)
UseTagsHis and T7 tagExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTRY-YFP
Plasmid#15302DepositorInsertYFP
UseGateway entryTagsExpressionMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCPP5537
Plasmid#128730PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertHopAO1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cdk6-DN-HA
Plasmid#1869DepositorInsertCdk6 (CDK6 Human)
UseTagsHAExpressionMammalianMutationDominant negative, D to N in conserved KLAD*FGLAR…PromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV14-mULK1
Plasmid#24301DepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCPP5099
Plasmid#128714PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopK1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5328
Plasmid#128721PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopU1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5306
Plasmid#128712PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopH1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5571
Plasmid#128713PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopI1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5539
Plasmid#128728PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAF1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5618
Plasmid#128709PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopE1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5617
Plasmid#128706PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopB1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-erHRP(N175S mutant)
Plasmid#79909PurposeerHRP(N175S mutant) pCMV backboneDepositorInserterHRP(N175S mutant)
UseTagsHA tagExpressionMammalianMutationN175S mutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_Ub
Plasmid#172134PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH3626
Plasmid#179630PurposePacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagsRFPExpressionWormMutationPromoterPacr-2(s)Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-hsDicer (D1709A)
Plasmid#41588DepositorInserthsDicer (DICER1 Human)
UseTagsFlagExpressionMammalianMutationD1709APromoterCMVAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
EYFP NES visual barcode
Plasmid#158676Purposetagging clonal populations with NES EYFP fluorescent protein. Adding NES to EYFP WCDepositorInsertEYFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only