164,737 results
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Plasmid#163846PurposeIn vitro transcription of TurboID-EGFP. Parton lab clone JRSDepositorInsertTurboID
UseIn vitro transcription of rnaTagsEGFPExpressionMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF6-myc-TBC1D15
Plasmid#79148Purposemammalian expression of TBC1D15DepositorInsertTBC1D15 (Tbc1d15 Mouse)
UseTagsHis, Myc, and V5ExpressionMammalianMutationS616N (please see depositor comment below)PromoterEF-1alphaAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-double floxed-eNpHR-EYFP-WPRE-pA (AAV9)
Viral Prep#20949-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-double floxed-eNpHR-EYFP-WPRE-pA (#20949). In addition to the viral particles, you will also receive purified pAAV-double floxed-eNpHR-EYFP-WPRE-pA plasmid DNA. EF1a-driven, cre-dependent eNpHR EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 ZoAl N-1F
Plasmid#203763PurposeFor expression of N-flag tagged ZoAl R2 protein in mammalian cellsDepositorInsertR2 retroelement protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mRuby2-pBAD
Plasmid#54771PurposeLocalization: Protein Expression Vector , Excitation: 559, Emission: 600DepositorTypeEmpty backboneUseTags6xHis and mRuby2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-dCas9-FLp300
Plasmid#61356Purposeencodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tcf/Lef:H2B-GFP
Plasmid#32610DepositorInsertTcf/Lef:H2B-GFP
UseTagsExpressionMutationPromoterhsp68Available sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 IRF7(5āUTR)-FF
Plasmid#85490PurposeFirefly luciferase under the control of IRF7 5'UTRDepositorInsert5'UTR of IRF7 (Irf7 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLARRYv2-EGFP
Plasmid#233208PurposeCloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP markerDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
TCARZ-CBh-GFP-SV40PA-GF3-R4A22
Plasmid#203767PurposeFor production of template RNA for use in PRINT, GFP under CBh promoterDepositorInsertPRINT template RNA for GFP
UseIvt templateTagsExpressionMutationPromoterCBhAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
Plasmid#207860PurposeAAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterA. nidulans gpdA promoter with Hammerhead ribozymā¦Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF1106
Plasmid#143762PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEPAS1 (EPAS1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)
Plasmid#204619PurposeExpression vector of Cascade and Cas3. Puromycin selectable.DepositorInsertCas7, Cas5, Cas8, Cas11, Cas6, and Cas3
UseCRISPRTagsEach protein has C- and N-terminal SV40 NLSExpressionMutationPromoterEF1aAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AckRS
Plasmid#137976Purposesequence optimized Nāacetyl lysylātRNA synthetase with cognate tRNA for genetic code expansionDepositorInsertAckRS and pylTcua
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-MNase
Plasmid#136291PurposeExpresses Micrococcal nucleaseDepositorInsertMNase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
BPK3082
Plasmid#78742PurposeHuman expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorInsertLbCpf1 crRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_FL
Plasmid#157867Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid proteinDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-Keratin-17
Plasmid#56465PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 488, Emission: 507DepositorInsertKeratin (KRT18 Human)
UseTagsmEGFPExpressionMammalianMutationV282LPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pEGFP-C1-MKLP1
Plasmid#70145Purposemammalian expression of MKLP1 with an eGFP fusionDepositorInsertMKLP1 (KIF23 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWB411
Plasmid#74805PurposeGateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL3718 (pQCascade_Pse)
Plasmid#200899PurposeExpresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.DepositorInsertPseCas7, PseCas8, PseCas6, PseTniQ
UseTagsNLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Beta-2-adrenergic receptor-mRFP
Plasmid#67145PurposeBeta-2-adrenergic receptor tagged with mRFP on carboxyl terminusDepositorInsertBeta-2-adrenergic receptor-mRFP (ADRB2 Human, Discosoma sp.)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJC175e
Plasmid#79219PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPPSP gIII, luxAB
UseTagsExpressionBacterialMutationPromoterPPSPAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD3
Plasmid#20185DepositorInsertNotch 3 Intracellular Domain (Notch3 Mouse)
UseTags3X FlagExpressionMammalianMutationContains murine Notch 3 Intracellular Domain (Metā¦PromoterCMVAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCBā
Plasmid#162703PurposepCB reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertpHnCB10 derived carboxysome operon, prk
UseTagsExpressionBacterialMutation5513T>G (tetR E37A); 7758G>T (second tet opā¦PromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE25
Plasmid#90367PurposeNotch - NIDC/CBF1 gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterNotchAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TFEBS211A-MYC (#805)
Plasmid#99957Purposemammalian expressionDepositorInsertTFEB (TFEB Human)
UseTags6xHIs and MycExpressionMammalianMutationS211APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-flex-iGluSnFR4f-NGR-WPRE
Plasmid#234442PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4f-NGR
UseAAV and Cre/LoxTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianMutationPromoterSynapsinAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330S-2
Plasmid#58778PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
nesprin TSmod 25
Plasmid#125555PurposeNesprin-2G force sensor with cerulean-F25-venus TSmoduleDepositorInsertmini-nesprin-2G (Syne2 Mouse)
UseTagscerulean, venusExpressionMammalianMutationTSmod changed from mTFP to cerulean, flageliform ā¦PromoterCMVAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (AAV PHP.eB)
Viral Prep#104495-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (#104495). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE plasmid DNA. CAG-driven, Cre-dependent GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpRYc-P2A-EGFP
Plasmid#208336PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYcDepositorInserthuman codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationSc++ (1-1119), SpRY (1111-1368), D10APromoterCAGAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
HRV-B3 IRES
Plasmid#189969PurposePart 2 plasmid to introduce the HRV-B3 IRES into circRNAsDepositorInsertHRV-B3 IRES
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-2.1 R
Plasmid#18723DepositorInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Shen Lab DdCBE assembly kit
Plasmid Kit#1000000212PurposeThe DddA-derived cytosine base editor (DdCBE) assembly kit is a collection of 192 modules harboring repeat variable di-residue (RVD) arrays for any desired target.DepositorAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Tmem192-mRFP-3xHA
Plasmid#134631Purposebait for affinity-isolation of lysosomesDepositorInsertTMEM192 (TMEM192 Human)
UseLentiviralTags3xHA, TEV, and mRFPExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE
Plasmid#198516PurposeExpresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.DepositorHas ServiceAAV1 and AAV5InsertPdCO
UseAAV and Cre/LoxTagsRho1D4 and mScarletExpressionMammalianMutationPromoterEF-1aAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVi U6_gRNA CMV_SadCas9-KRAB
Plasmid#214609PurposeAll-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassetteDepositorInsertsgRNA
SadCas9
UseAAVTagsNP NLS and SV40 NLSExpressionMammalianMutationD10A, N580APromoterCMV and U6Available sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-polyQ23-NLS
Plasmid#231891PurposeExpression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleusDepositorInsertHTT partial exon 1 Q23-NLS (HTT Human)
UseTagsEGFP and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
A547 (miR-146a promoter)
Plasmid#15091DepositorInsertmiR-146a promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_r5-HT1.0
Plasmid#208719PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in neuronsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP1-SB100X
Plasmid#154887PurposeSleeping Beauty transposase fused to SP1DepositorInsertSP1-Sleeping Beauty
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pITGA4-GFP
Plasmid#81178PurposeMammalian expression of human full length ITGA4-EGFPDepositorInsertITGA4 (ITGA4 Human)
UseTagsEGFPExpressionMammalianMutationContains KLGFFKR motif; A1002L (please see deposiā¦PromoterCMVAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVCT-tTR-KRAB
Plasmid#11643PurposeTet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertCAG promoters, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
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Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem
Plasmid#229795PurposeBxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2DepositorInsertTRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hATG7wt
Plasmid#87867PurposeExpress a wild type human Atg7/Apg7 in mammalian cellsDepositorInsertATG7 (ATG7 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Golgi-7
Plasmid#58018PurposeLocalization: Golgi Complex, Excitation: 555, Emission: 584DepositorInsertGolgi (B4GALT1 Human)
UseTagsmTagRFPExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits