167,785 results

1. EGFP-Peroxisomes-0

   Plasmid

   #54501

   Purpose

   Localization: Peroxisomes, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Type

   Empty backbone

   Tags

   EGFP and Peroximal Targeting Signal 1 (PTS1)

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. px458_2A_GFP_sgRNA_LTN1

   Plasmid

   #127125

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA LTN1 (LTN1 Human)
   

   Use

   CRISPR

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-GFP-AURKA-mCherry

   Plasmid

   #99876

   Purpose

   Expression of GFP-AURKA-mCherry for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP and mCherry

   Expression

   Bacterial

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. EcSTH

   Plasmid

   #211923

   Purpose

   This plasmid contains human codon optimized sequence of EcSTH which can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio in mammalian cells.

   Depositor

   Binghui Li

   Article

   Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

   Insert

   EcSTH
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mNeonGreen-APEX3

   Plasmid

   #202590

   Purpose

   Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

   Depositor

   Reuben Harris

   Article

   Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

   Insert

   mNeonGreen-APEX3-V5 (APX1 )
   

   Use

   Retroviral

   Tags

   V5 epitope and mNeonGreen

   Expression

   Mammalian

   Mutation

   APEX2 L242A

   Promoter

   MESV LTR

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-V2-ORF

   Plasmid

   #73636

   Purpose

   Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Tags

   Venus fragment 2 (V2)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-dusp6

   Plasmid

   #82584

   Purpose

   5' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cells

   Depositor

   Kryn Stankunas

   Article

   Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

   Insert

   FGF-responsive element of the dusp6 promoter
   

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP13998 - pAAV-AiE0390m-minBG-iCre(R297T)-BGHpA (Alias: CN3998)

   Plasmid

   #208162

   Purpose

   AiE0390m is an enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCreR297T
   

   Use

   AAV

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. human p53-(1-393)

   Plasmid

   #24859

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Full length.

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGST1-TOM1-VHS

    Plasmid

    #44423

    Depositor

    James Hurley

    Article

    Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

    Insert

    TOM1-VHS (1-152) (TOM1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pJA54 HLA-A*03:01

    Plasmid

    #180451

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*03:01
    

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCk1

    Plasmid

    #136695

    Purpose

    Acceptor plasmid for odd level position 1, lacZ blue-white screening. Part of the OpenPlant toolkit.

    Depositor

    Nicola Patron

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ACBE-Q

    Plasmid

    #204608

    Purpose

    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG/ATP9A-HA

    Plasmid

    #209213

    Purpose

    Mammalian expression of ATP9A

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP9A (ATP9A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. DLG1_PDZ_3

    Plasmid

    #103917

    Purpose

    Protein expression and purification of DLG1 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG1 (DLG1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. RV-mCD4-HH5-5

    Plasmid

    #124985

    Purpose

    Express the Helicobacter hepaticus specific TCRa/b HH5-5 in T cell hybridoma cells

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH5-5 TCRa P2A TCRb
    

    Use

    retroviral

    Expression

    Mammalian

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. DLG1_PDZ_2

    Plasmid

    #103916

    Purpose

    Protein expression and purification of DLG1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG1 (DLG1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. DLG1_PDZ_1

    Plasmid

    #103915

    Purpose

    Protein expression and purification of DLG1 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG1 (DLG1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PDZK1_PDZ_1

    Plasmid

    #103927

    Purpose

    Protein expression and purification of PDZK1 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PDZK1_PDZ_4

    Plasmid

    #103945

    Purpose

    Protein expression and purification of PDZK1 PDZ4 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDONR221_SLC35C1

    Plasmid

    #132063

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC35C1 (SLC35C1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. 4QQG

    Plasmid

    #61948

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HTATIP:JMC001-B06:C41570 (KAT5 Human)
    

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pAAVS1-PDi-CRISPRn

    Plasmid

    #73500

    Purpose

    Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    Cas9
    rtTA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CAG and TRE

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. TJP2_PDZ_3

    Plasmid

    #103949

    Purpose

    Protein expression and purification of X104 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP2 (TJP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TJP2_PDZ_2

    Plasmid

    #103955

    Purpose

    Protein expression and purification of X104 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP2 (TJP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (AAV8)

    Viral Prep

    #130909-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (#130909). In addition to the viral particles, you will also receive purified AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] plasmid DNA. CAG-driven, Flp-dependent expression of ChrimsonR-tdTomato. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    CAG

    Tags

    tdTomato (Flp-dependent)

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

27. pEIGW roGFP2-Orp1

    Plasmid

    #64993

    Purpose

    Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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28. LB1554 (Bcl-2 promoter with MHS1234, Cdx mut)

    Plasmid

    #15384

    Depositor

    Linda Boxer

    Article

    Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

    Insert

    B-cell lymphoma/leukemia-2 (BCL2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Bacterial and Mammalian

    Mutation

    Bcl-2 full promoter with IgH full enhancer MHS123…

    Available Since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

29. pCMV-ABE8e-eVRQR-P2A-EGFP (HES1425)

    Plasmid

    #242657

    Purpose

    CMV promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with neVRQR(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    pCMV-ABE8e-SpCas9-VRQR(S55R)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA, eVRQR mutations in SpCas…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pGEM-T_mouseH3.1-HaloTag

    Plasmid

    #244242

    Purpose

    CRISPR/Cas9 donor plasmid to tag mouse histone H3.1 with Halo tag

    Depositor

    Kazuhiro Maeshima

    Article

    Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

    Insert

    H3.1 (H3c1 Mouse)
    

    Use

    CRISPR

    Tags

    HaloTag

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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