163,703 results

1. pCAG-D1R

   Plasmid

   #89160

   Purpose

   Express vaccinia virus D1R in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D1R
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promotor

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCMV-SS-antiFLAGscFv-TM

   Plasmid

   #198303

   Purpose

   Displays anti-FLAG scFv from the surface of mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pHCMV-SS-antiFLAGscFv-TM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL2680

   Plasmid

   #85581

   Purpose

   Replicating base vector for constructing cpf1/CRISPR editing plasmids

   Depositor

   Himadri Pakrasi

   Article

   Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBA950

   Plasmid

   #122239

   Purpose

   CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiGuide-puro-NLS-GFP

   Plasmid

   #185473

   Purpose

   Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCGN-ATF6 (1-373)

   Plasmid

   #27173

   Purpose

   For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

   Depositor

   Ron Prywes

   Article

   Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

   Insert

   ATF6 (ATF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Contains only aa 1-373 (please see depositor comm…

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCXLE-Sox2-17-T2A-Klf4-E2A-Myc

   Plasmid

   #210016

   Purpose

   episomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve reset

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   CAG

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-human Igf2bp2-IRES-GFP

   Plasmid

   #91890

   Purpose

   Retroviral expression of human Igfbp2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Igf2bp2 (IGF2BP2 Human)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Luciferase

   Plasmid

   #213979

   Purpose

   Expresses Doxy Inducible Luciferase

   Depositor

   Coleman Lindsley

   Article

   Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   6xHis affinity tag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Tet-responsive promoter PTight, consisting of sev…

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-Merlin

    Plasmid

    #84293

    Purpose

    Expression of wt Merlin

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEHAC1-HA-BirA-GSGS-Traf6

    Plasmid

    #232586

    Purpose

    Express BirA tagged to the N-terminus of TRAF6 with a GSGS linker between BirA and TRAF6.

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    BirA -TRAF6 (TRAF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-MetGFP

    Plasmid

    #37560

    Depositor

    David Rimm

    Insert

    MET (MET Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ub-M-GFP

    Plasmid

    #11938

    Depositor

    Nico Dantuma

    Article

    Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

    Insert

    Ub-M-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Methionine …

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriEx-mCerulean-PA-Rac1

    Plasmid

    #22030

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His and mCerulean

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cyto-roGFP

    Plasmid

    #49435

    Purpose

    Expresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cells

    Depositor

    Paul Schumacker

    Article

    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

    Insert

    roGFP2
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    C48S, Q80R, S147C, and Q204C in Clontech's G…

    Promoter

    CMV

    Available since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. 4xmts-mScarlet-I

    Plasmid

    #98818

    Purpose

    In vivo visualization of mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    mts
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYLCRISPR/Cas9Pubi-B

    Plasmid

    #66188

    Purpose

    expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    maize ubiquitin promoter (Pubi)

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-FLIP-puro-dsRed-GFP-miRNA

    Plasmid

    #32704

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Insert

    emGFP-miRNA
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-rtTA

    Plasmid

    #163601

    Purpose

    Expresses rtTA in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    rtTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PGC-1 alpha promoter 2kb luciferase

    Plasmid

    #8887

    Depositor

    Bruce Spiegelman

    Article

    Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

    Insert

    PGC-1 alpha promoter (Ppargc1a Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSBBi-Pur LaG17-synNotch-TetRVP64

    Plasmid

    #158133

    Purpose

    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

    Depositor

    Neal Devaraj

    Insert

    LaG17-synNotch-TetRVP64
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDonor_ShCAST_kanR

    Plasmid

    #127924

    Purpose

    Kanamycin resistance donor for ShCAST

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-TRE-DIO-ChR2-TdTomato (Cre-OFF)

    Plasmid

    #166610

    Purpose

    Encodes Cre-inactivated ChR2-TdTomato under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    ChR2-TdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    H134R

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSET.HaloTag.v7

    Plasmid

    #226197

    Purpose

    bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    HaloTag.v7
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. shh10

    Plasmid

    #64867

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

    Depositor

    John Flannery, David Schaffer

    Article

    Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

    Insert

    shh10 cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    I319V, N451D, D532N

    Promoter

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEP4 E02S ET2K

    Plasmid

    #20927

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; SV40LT and Klf4 (SOX2, POU5F1, KLF4 Human)
    

    Use

    Episomal/ebna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLI50

    Plasmid

    #13573

    Depositor

    Chia Lee

    Article

    Lee et al Gene. 1991 Jul 15. 103(1):101-5.

    Type

    Empty backbone

    Use

    S. aureus-e. coli shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

    Plasmid

    #169128

    Purpose

    AAV plasmid containing a mKate2 and a truncated PDE4D3

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Insert

    mKate2-PDE4DCatL (PDE4D Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    truncated, Catalatic domain only

    Promoter

    EF1a

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. iF53 PB-Zim3-dCas9-mScarlet-Hygro

    Plasmid

    #204726

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSTARR-seq_human

    Plasmid

    #71509

    Purpose

    humanSTARR-seq screening vector with SCP1 core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

    Type

    Empty backbone

    Use

    Humanstarr-seq screening vector

    Tags

    sgGFP (SuperGlo, Qbiogene, Inc)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

31. c-Fos

    Plasmid

    #187905

    Purpose

    Expresses c-Fos in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    cFos (FOS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-Ef1a-DIO ChETA-EYFP (AAV5)

    Viral Prep

    #26968-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO ChETA-EYFP (#26968). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO ChETA-EYFP plasmid DNA. EF1a-driven, Cre-dependent, ChETA fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

    Promoter

    EF1a

    Tags

    EYFP (Cre-dependent)

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

33. gWiz_BM40-vGAT-VL-hIgG1-CL

    Plasmid

    #218437

    Purpose

    mammalian expression plasmid for the light chain fragment of an anti-vGAT Fab domain

    Depositor

    Axel Brunger

    Article

    Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

    Insert

    vGAT-VL-hIgG1-CL
    

    Use

    Affinity Reagent/ Antibody

    Tags

    BM40

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. gWiz_BM40-vGAT-VH-hIgG1-CH1-TS-HIS

    Plasmid

    #218436

    Purpose

    mammalian expression plasmid for the heavy chain fragment of an anti-vGAT Fab domain

    Depositor

    Axel Brunger

    Article

    Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

    Insert

    vGAT-Vh-hIgG1-CH1
    

    Use

    Affinity Reagent/ Antibody

    Tags

    BM40 and TwinStrep-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDONR-A-HYG

    Plasmid

    #29635

    Depositor

    Scott Gold, David_L Andrews

    Article

    Garcia-Pedrajas et al Fungal Genet Biol. 2008 Apr . 45(4):379-

    Type

    Empty backbone

    Use

    Gateway donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. HA-KIF5B*-strep

    Plasmid

    #120170

    Purpose

    Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5B (Kif5b Mouse)
    

    Use

    Tags

    HA and Streptavidin

    Expression

    Mammalian

    Mutation

    Truncated KIF5B: 1-555

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. px459-TDP 43 sgRNA2

    Plasmid

    #133763

    Purpose

    Expresses Cas9 and human TDP-43 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHyperTEV60

    Plasmid

    #228496

    Purpose

    10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

    Depositor

    Richard Baker

    Insert

    TEV Protease
    

    Use

    Tags

    10xHis-SUMO(Smt3)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pminiTol2

    Plasmid

    #31829

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge