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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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173,387 results

Showing: 9401 - 9440 of 173387 results

  2. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

    Plasmid
    #71293
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Article
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Tags
    FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71293-plasmid-map-sequence-id-127936

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  3. pAAV.GFA104.PI.eGFP.WPRE.bGH (AAV5)

    Viral Prep
    #100896-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.GFA104.PI.eGFP.WPRE.bGH (#100896). In addition to the viral particles, you will also receive purified pAAV.GFA104.PI.eGFP.WPRE.bGH plasmid DNA. GFA104-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Philip Haydon
    Article
    Ortinski et al Nat Neurosci. 2010 May;13(5):584-91. doi
    Promoter
    GFA104 (GFAP promoter)
    Tags
    EGFP
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

    Plasmid
    #181974
    Purpose
    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Article
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)
    Use
    Lentiviral
    Mutation
    NGFR truncation: aa 11-276
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    181974-plasmid-map-sequence-id-355578

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  13. pET21 MBPx-SpyCatcher

    Plasmid
    #72327
    Purpose
    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET21 MBPx-SpyCatcher
    Tags
    N-terminal His6 tag
    Expression
    Bacterial
    Mutation
    MBP contains A312V, I317V mutations and 172, 173,…
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72327-plasmid-map-sequence-id-434061

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  18. pcDNA3-TLR9-YFP

    Plasmid
    #13642
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 9 (TLR9 Human)
    Tags
    YFP
    Expression
    Mammalian
    Available Since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13642-plasmid-map-sequence-id-392328

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  31. TFORF3298

    Plasmid
    #144774
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    EZH2 (EZH2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144774-plasmid-map-sequence-id-404378

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  33. pOpen-TthpolA

    Plasmid
    #165533
    Purpose
    DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Tth DNA polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165533-plasmid-map-sequence-id-339122

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  35. pPEPX-P3-sgRNAluc

    Plasmid
    #85590
    Purpose
    Integrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template for
    Depositor
    Jan-Willem Veening
    Article
    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do
    Insert
    sgRNA targeting firefly luciferase encoding gene
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    P3
    Available Since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85590-plasmid-map-sequence-id-169513

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Showing: 9401 - 9440 of 173387 results