Skip to main content
Addgene
Showing: 1201 - 1250 of 159762 results

  2. CAG-Cremyc-2A-GFP

    Plasmid
    #116879
    Purpose
    Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.
    Depositor
    Qiang Lu
    Article
    Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:
    Insert
    Cremyc-2A-GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    116879-plasmid-map-sequence-id-243398

    Enlarge

  3. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV9)

    Viral Prep
    #20298-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  4. TFORF1373

    Plasmid
    #143954
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF143 (ZNF143 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143954-plasmid-map-sequence-id-404612

    Enlarge

  7. pLV-SFFV-PIK3CA-WPRE-UbC-Emerald

    Plasmid
    #203797
    Purpose
    Lentiviral vector expressing human phosphoinositide 3-kinase catalytic subunit alpha (PIK3CA) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Article
    Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d
    Inserts
    PIK3CA (PIK3CA Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203797-plasmid-map-sequence-id-401572

    Enlarge

  20. pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

    Plasmid
    #192939
    Purpose
    FlpO-based digitizer circuit driven by AP1 promoter
    Depositor
    Wilson Wong
    Article
    Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:
    Inserts
    AP1(2)/minTK promoter
    FlpO-ABI
    PYL-FlpO
    CAG promoter
    frt-STOP-frt
    GFP
    luciferase
    pPGK
    Zeocin resistance
    BFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    192939-plasmid-map-sequence-id-380399

    Enlarge

  24. TFORF1374

    Plasmid
    #143955
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF143 (ZNF143 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143955-plasmid-map-sequence-id-404613

    Enlarge

  29. TFORF2020

    Plasmid
    #142313
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYBL2 (MYBL2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142313-plasmid-map-sequence-id-395784

    Enlarge

  30. pDONR221-SLC25A5_STOP

    Plasmid
    #161173
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC25A5 (SLC25A5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161173-plasmid-map-sequence-id-398139

    Enlarge

  31. pCWX-R4DESTR2-PGK-BSD

    Plasmid
    #114320
    Purpose
    Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Blasticidin resistance
    Depositor
    Patrick Salmon
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114320-plasmid-map-sequence-id-219986

    Enlarge

  36. WDR5 (2H9N)

    Plasmid
    #25229
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5_01 (WDR5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  41. TFORF1372

    Plasmid
    #143953
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF143 (ZNF143 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    143953-plasmid-map-sequence-id-471144

    Enlarge

  42. MT-AvrXa10-FT

    Plasmid
    #234373
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus
    Depositor
    Michael Smanski
    Article
    Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202
    Inserts
    NbGP41-GFP-AvrXa10-GB1
    dCas9-24XGP41
    gRNAs targeting FT locus
    RFP
    Use
    Synthetic Biology
    Tags
    GB1, GFP, and GP41
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsiā€¦
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234373-plasmid-map-sequence-id-469429

    Enlarge

  44. pJL31

    Plasmid
    #227428
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)
    Depositor
    Pierre Santucci
    Insert
    mWasabi
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227428-plasmid-map-sequence-id-455967

    Enlarge

  45. pJL32

    Plasmid
    #227429
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)
    Depositor
    Pierre Santucci
    Insert
    tdTomato
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227429-plasmid-map-sequence-id-455968

    Enlarge

  46. pJL33

    Plasmid
    #227430
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)
    Depositor
    Pierre Santucci
    Insert
    mWasabi
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227430-plasmid-map-sequence-id-455969

    Enlarge

  47. pJL34

    Plasmid
    #227431
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)
    Depositor
    Pierre Santucci
    Insert
    tdTomato
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227431-plasmid-map-sequence-id-455970

    Enlarge

  50. AiP13790 - pAAV-AiE0475m-minBG-iCre(R297T)-BGHpA (Alias: CN3790)

    Plasmid
    #230384
    Purpose
    AiE0475m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.
    Insert
    iCre(R297T)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    minBG
    Available since
    Feb. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    230384-plasmid-map-sequence-id-462402

    Enlarge

Showing: 1201 - 1250 of 159762 results