We narrowed to 60,538 results for: Gene
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Plasmid#38191DepositorAvailable SinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only
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pCas
Plasmid#62225PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRDepositorHas ServiceCloning Grade DNAInsertcas9
UseCRISPR; Lambda red recombinaseExpressionBacterialPromoternative cas9 promoterAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
EcNR2 Strain
Bacterial Strain#26931DepositorBacterial ResistanceChloramphenicol and AmpicillinAvailable SinceJan. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CreERT2
Plasmid#14797PurposeMammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)DepositorInsertCre-ERT2 fusion protein
UseCre/LoxExpressionMammalianPromoterCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
VP12
Plasmid#72247PurposeHuman expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A and Q926APromoterCMVAvailable SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP
Plasmid#11150PurposeMammalian expression vector for expression of GFP (CAG promoter)DepositorHas ServiceCloning Grade DNAInsertEGFP from Aequorea victoria
Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hOCT3/4-shp53-F
Plasmid#27077PurposeIntegration-free (episomal) expression of human OCT3/4 and shRNA against p53DepositorInsertOCT3/4 (POU5F1 Human)
ExpressionMammalianAvailable SinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hSK
Plasmid#27078PurposeIntegration-free (episomal) expression of human SOX2 and KLF4DepositorAvailable SinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hUL
Plasmid#27080PurposeIntegration-free (episomal) expression of human L-MYC and LIN28DepositorAvailable SinceMarch 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
E-cadherin-GFP
Plasmid#28009DepositorAvailable SinceMarch 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pH2B-mNG2(11)
Plasmid#206043PurposePlasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.DepositorInsertH2B gene fused to the mNG2(11) fragment
UseSynthetic BiologyTagsH2B-mNG2(11)ExpressionMammalianPromoterCMVAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV Retrograde)
Viral Prep#37825-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCAS9-VP64_GFP
Plasmid#61422PurposeExpresses dCAS9-VP64 activator with 2A GFPDepositorHas ServiceConcentrated Lentiviral PrepInsertdCAS9(D10A,H840A)-VP64_2A_GFP
UseCRISPR and LentiviralExpressionMammalianMutationD10A and H840A in Cas9PromoterEF1AAvailable SinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
Plasmid#71236PurposeExpress sgRNA and dCas9-KRAB from lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A Puro
sgRNA
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-tTA (AAV1)
Viral Prep#99120-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-ihSyn1-tTA (#99120). In addition to the viral particles, you will also receive purified pAAV-ihSyn1-tTA plasmid DNA. Expression of the tet-off transactivator from an inducible synapsin promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterihSyn1Available SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42229PurposeThis plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianAvailable SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-KRAB
Plasmid#50917PurposeTet-regulatable dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterTREAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX-sgRNA
Plasmid#50662PurposeLentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)DepositorInsertsgAAVS1
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE3
Plasmid#73021PurposeExpresses BE3 in mammalian cellsDepositorInsertBE3
ExpressionMammalianPromoterCMVAvailable SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.GFP
Plasmid#57818PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGPromoterEFS and hU6Available SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-FLAG-Gene33
Plasmid#21633DepositorAvailable SinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
FUGW
Plasmid#14883Purpose3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresionDepositorInsertflap-Ub promoter-GFP-WRE
UseLentiviralExpressionMammalianAvailable SinceMay 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
B. subtilis Single Gene Deletion Library--Kanamycin
Plasmid Kit#1000000115PurposeA collection of Bacillus subtilis strains in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.DepositorApplicationStrain CollectionsVector TypeBacterial ExpressionAvailable SinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-GFP
Plasmid#132776PurposePrime editing in mammalian cells with co-translational GFP expressionDepositorInsertPE2-P2A-GFP
TagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato (AAV9)
Viral Prep#28306-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available SinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTargetF
Plasmid#62226PurposeConstitutive expression of sgRNA without donor editing template DNADepositorInsertsgRNA
UseCRISPRExpressionBacterialPromoterpij23119Available SinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-mCherry
Plasmid#99154PurposeLentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.DepositorInsertsCas9
mCherry
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS-NSAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato (AAV Retrograde)
Viral Prep#28306-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. Cre-dependent (FLEX) tdTomato expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available SinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP
Plasmid#71237PurposeExpress sgRNA and dCas9-KRAB from 3rd generation lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A GFP
sgRNA
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CRISPR_DpmrA
Plasmid#160903PurposeCRISPR_Cas9, lambda red recombineeringDepositorInsertsCas9
Lambda Red Recombineering Genes
Zeocin Resistance Cassette
PromoteraraBADAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only