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179,463 results

Showing: 101 - 150 of 179463 results

  1. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47 Articles
    74223-plasmid-map-sequence-id-334595

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  4. pLV-mCherry

    Plasmid
    #36084
    Purpose
    3rd generation lentiviral plasmid expressing mCherry
    Depositor
    Pantelis Tsoulfas
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    June 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91 Articles
    36084-plasmid-map-sequence-id-372931

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  5. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV9)

    Viral prep
    #44362-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV9)

    Viral prep
    #44361-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV5)

    Viral prep
    #20298-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  11. pAAV-hSyn-EGFP (AAV Retrograde)

    Viral prep
    #50465-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. Synapsin-driven EGFP-expression control. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only

  12. pLKO.1 hygro

    Plasmid
    #24150
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
    Depositor
    Bob Weinberg
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 for shRNA
    Available since
    March 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    76 Articles
    24150-plasmid-map-sequence-id-376525

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  14. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV2)

    Viral prep
    #44362-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Nov. 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. lentiCas9-Blast (Lentiviral Prep)

    Viral prep
    #52962-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.
    Depositor
    Feng Zhang
    Publication
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Promoter
    EFS-NS
    Available since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pLenti CMV rtTA3 Blast (w756-1)

    Plasmid
    #26429
    Purpose
    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast
    Depositor
    Eric Campeau
    Insert
    Tetracycline repressor A3 mutant
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Jan. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63 Articles
    26429-plasmid-map-sequence-id-354259

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  20. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55 Articles
    61357-plasmid-map-sequence-id-358013

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  21. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV2)

    Viral prep
    #44361-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Nov. 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  31. pBOB-EF1-FastFUCCI-Puro

    Plasmid
    #86849
    Purpose
    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection
    Depositor
    Kevin Brindle, Duncan Jodrell
    Publication
    Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164
    Inserts
    mKO2-hCDT1(30-120)
    mAG-hGEM(1-110)
    pac (puromycin N-acetyl-transferase)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1 and PGK
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46 Articles
    86849-plasmid-map-sequence-id-237968

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  32. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    142 Articles
    61426-plasmid-map-sequence-id-140565

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  37. pAAV-hSyn-hM4D(Gi)-mCherry (AAV8)

    Viral prep
    #50475-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  38. pAAV-Ef1a-mCherry-IRES-Cre (AAV Retrograde)

    Viral prep
    #55632-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-mCherry-IRES-Cre (#55632). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry-IRES-Cre plasmid DNA. Cre and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a and Ef1a/IRES
    Tags
    mCherry
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only

  40. Human sgRNA library Brunello in lentiCRISPRv2

    Pooled library
    #73179
    Purpose
    Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls
    Depositor
    John Doench, David Root
    Publication
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Has service
    Lentiviral Prep
    Expression
    Mammalian
    Use
    CRISPR and Lentiviral
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    94 Articles

  43. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (AAV5)

    Viral prep
    #62723-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (#62723). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] plasmid DNA. Cre-dependent ChrimsonR-tdTomato under the control of the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Publication
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato (Cre-dependent)
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only

  45. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    146 Articles
    83480-plasmid-map-sequence-id-423158

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Showing: 101 - 150 of 179463 results