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161,492 results

Showing: 4751 - 4800 of 161492 results

  1. pCas9_GFP

    Plasmid
    #44719
    Purpose
    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    44719-plasmid-map-sequence-id-472945

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  3. pAAV.hSynapsin.SF-iGluSnFR.A184S (AAV1)

    Viral Prep
    #106174-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSynapsin.SF-iGluSnFR.A184S (#106174). In addition to the viral particles, you will also receive purified pAAV.hSynapsin.SF-iGluSnFR.A184S plasmid DNA. hSynapsin driven SF-iGluSnFR.A184S, glutamate sensor. The A184S mutant has a higher glutamate affinity. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Promoter
    Syn
    Tags
    None
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  4. mCherry-ZO1-C-14

    Plasmid
    #55165
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ZO1 (TJP1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55165-plasmid-map-sequence-id-194785

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  6. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid
    #197510
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Expression
    Mutation
    ABE8e mutations in TadA and nSpRY(D10A/A61R)
    Promoter
    Cbh
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197510-plasmid-map-sequence-id-386692

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  12. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV1)

    Viral Prep
    #51084-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting
    Promoter
    Syn
    Tags
    nuclear dTomato (physically separate, not a fusion protein)
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. mEmerald-Calreticulin-N-16

    Plasmid
    #54023
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Calreticulin (CALR Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54023-plasmid-map-sequence-id-474311

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  17. Epac-S-H81

    Plasmid
    #170339
    Purpose
    GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Insert
    GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170339-plasmid-map-sequence-id-344640

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  18. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV5)

    Viral Prep
    #105540-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Expression of EGFP-Cre from hSyn promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    EGFP
    Available since
    May 26, 2020
    Availability
    Academic Institutions and Nonprofits only

  20. pYD1

    Plasmid
    #73447
    Purpose
    expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells
    Depositor
    Dane Wittrup
    Article
    Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.
    Type
    Empty backbone
    Use
    Tags
    6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    73447-plasmid-map-sequence-id-364550

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  22. pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 (AAV1)

    Viral Prep
    #172922-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 (#172922). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 plasmid DNA. Syn-driven expression of calcium sensor jGCaMP8m in axons with cytosolic expression of mRuby3. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Promoter
    Syn
    Tags
    mRuby3
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only

  24. pCI-ING3

    Plasmid
    #79070
    Purpose
    Expression of p47 ING3 in mammalian cells
    Depositor
    Karl Riabowol
    Insert
    ING3 (p47 ING3 isoform) (ING3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. pAAV-Syn-ChR2(H134R)-GFP (AAV Retrograde)

    Viral Prep
    #58880-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Syn-ChR2(H134R)-GFP (#58880). In addition to the viral particles, you will also receive purified pAAV-Syn-ChR2(H134R)-GFP plasmid DNA. Humanized channelrhodopsin H134R mutant fused to GFP, under the control of the Synapsin promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.
    Promoter
    Syn
    Tags
    GFP
    Available since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only

  29. ACBE-Q

    Plasmid
    #204608
    Purpose
    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204608-plasmid-map-sequence-id-404717

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  37. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV Retrograde)

    Viral Prep
    #162378-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Tags
    None
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  44. TFORF1636

    Plasmid
    #141856
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HOXC13 (HOXC13 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141856-plasmid-map-sequence-id-389795

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  47. pAAV-hSynapsin-FLEX-soCoChR-GFP (AAV9)

    Viral Prep
    #107712-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSynapsin-FLEX-soCoChR-GFP (#107712). In addition to the viral particles, you will also receive purified pAAV-hSynapsin-FLEX-soCoChR-GFP plasmid DNA. Synapsin-driven, Cre-dependent somatic CoChR (soCoChR) fused to GFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.
    Promoter
    Syn
    Tags
    GFP (Cre-dependent)
    Available since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  50. pDONR221-SLC20A1_STOP

    Plasmid
    #161176
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC20A1 (SLC20A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161176-plasmid-map-sequence-id-333109

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Showing: 4751 - 4800 of 161492 results