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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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173,361 results

Showing: 3881 - 3920 of 173361 results

  1. mCherry-Rab11a-7

    Plasmid
    #55124
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab11a (RAB11A Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55124-plasmid-map-sequence-id-127231

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  3. pETDuet_huCAD_ICADL (TevS)

    Plasmid
    #100098
    Purpose
    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences
    Depositor
    Jim Wells
    Article
    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112
    Insert
    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    Expression
    Bacterial
    Mutation
    two caspase cleavage sites (DETD-117 and DAVD-224…
    Promoter
    T7
    Available Since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    100098-plasmid-map-sequence-id-261413

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  13. pKSE401

    Plasmid
    #62202
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62202-plasmid-map-sequence-id-288285

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  20. tdTomato-C1

    Plasmid
    #54653
    Purpose
    Localization: C1 Cloning Vector, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    tdTomato
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54653-plasmid-map-sequence-id-450928

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  21. pEVOL-pBpF

    Plasmid
    #31190
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.
    Depositor
    Peter Schultz
    Article
    Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99
    Insert
    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    Expression
    Bacterial
    Mutation
    Y32G, E107P, D158T, I159S
    Available Since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31190-plasmid-map-sequence-id-418765

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  26. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171991-plasmid-map-sequence-id-338127

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  29. pCMVβ-p300-myc

    Plasmid
    #30489
    Purpose
    Mammalian expression of human p300 with myc tag
    Depositor
    Tso-Pang Yao
    Insert
    p300 (EP300 Human)
    Tags
    Myc
    Expression
    Mammalian
    Available Since
    Oct. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    30489-plasmid-map-sequence-id-244762

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  34. pLEX-MCS UBC promoter

    Plasmid
    #128045
    Purpose
    Lentiviral vector with UBC promoter
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    128045-plasmid-map-sequence-id-256398

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  40. pAAV-hSyn Con/Fon EYFP (AAV Retrograde)

    Viral Prep
    #55650-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon EYFP (#55650). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon EYFP plasmid DNA. Synapsin driven, Cre and Flp-dependent EYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    Syn
    Tags
    EYFP (Cre- and Flp-dependent)
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 3881 - 3920 of 173361 results