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Bacterial Expression

29,729 results

Showing: 5251 - 5300 of 29729 results

  1. pGEX6P1-DEST-Strep2

    Plasmid
    #119753
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.
    Depositor
    Martin Reijns, Andrew Jackson
    Type
    Empty backbone
    Use
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119753-plasmid-map-sequence-id-243311

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  2. PE-SUMO CRYAB

    Plasmid
    #217670
    Purpose
    For expression of SUMO tagged human alpha B crystallin
    Depositor
    Larry David
    Insert
    CRYAB (CRYAB Human)
    Use
    Tags
    6XHis and Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217670-plasmid-map-sequence-id-434869

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  6. KLHL11

    Plasmid
    #39019
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KLHL11 (KLHL11 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. p006-GFP-weak

    Plasmid
    #108314
    Purpose
    GFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFAB4282
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    108314-plasmid-map-sequence-id-220975

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  14. (SIM)5

    Plasmid
    #126947
    Purpose
    Bacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    unknown
    Available since
    Sept. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126947-plasmid-map-sequence-id-255788

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  16. GCN5L2

    Plasmid
    #25482
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GCN5L2 (KAT2A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pET32-sLatherin

    Plasmid
    #61685
    Purpose
    bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion
    Depositor
    Brian Smith
    Article
    Vance et al J R Soc Interface. 2013 Jun 19;10(85):20
    Insert
    Latherin (LATH horse)
    Use
    Tags
    HIS and Trx-Tag thioredoxin protein
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. pDIV300

    Plasmid
    #204968
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204968-plasmid-map-sequence-id-454124

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  22. pJMP12089

    Plasmid
    #222357
    Purpose
    Tn7 integrative vector with PabstBR promoter expression cassette and hygR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222357-plasmid-map-sequence-id-439068

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  24. pDIV313

    Plasmid
    #204956
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus fumigatus U3 promoter, Aspergillus ni…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204956-plasmid-map-sequence-id-415598

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  25. pBad-HPII

    Plasmid
    #105839
    Purpose
    expresses E. coli HPII catalase with N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105839-plasmid-map-sequence-id-205523

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  27. mCherry-OptoPB

    Plasmid
    #113935
    Purpose
    Express a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactions
    Depositor
    Yubin Zhou
    Article
    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi
    Insert
    OptoPB
    Use
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    113935-plasmid-map-sequence-id-367689

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  29. pcDNA.3.1.ADRB2-NP

    Plasmid
    #134376
    Purpose
    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    ADRB2-NP (ADRB2 Human)
    Use
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    Aug. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134376-plasmid-map-sequence-id-306396

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  32. pKM263-USP7

    Plasmid
    #196365
    Purpose
    protein expression of the GST-tagged catalytic domain
    Depositor
    Stefan Knapp
    Insert
    USP7 catalytic domain
    Use
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    contains only S207-E560
    Promoter
    T7
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196365-plasmid-map-sequence-id-386294

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  35. pOPINN-GFP

    Plasmid
    #53541
    Purpose
    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
    Depositor
    Ray Owens
    Insert
    GFP
    Use
    Tags
    N-His-GFP
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53541-plasmid-map-sequence-id-83419

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  36. PE-SUMO CRYBB1

    Plasmid
    #217675
    Purpose
    For expression of SUMO tagged human beta B1 crystallin
    Depositor
    Larry David
    Insert
    CRYBB1 (CRYBB1 Human)
    Use
    Tags
    6XHis and Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217675-plasmid-map-sequence-id-434867

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Showing: 5251 - 5300 of 29729 results