32,656 results
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Plasmid#49374DepositorInsertTetR repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTNT-B18R
Plasmid#58978Purposeexpresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
ExpressionBacterialPromoterT7/Sp6Available SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET23a-mUbc9
Plasmid#53137PurposeE.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)DepositorInsertUbc9 (Ube2i Mouse)
TagsuntaggedExpressionBacterialMutationprotein sequence is identical to humanPromoterT7Available SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
BARD1 26-327 pET28a
Plasmid#12646DepositorInsertBRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)
ExpressionBacterialAvailable SinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
p356_DG
Plasmid#234699PurposeGolden gate domesticated pET28b for scarless cloning with His10-MBP-3CDepositorTypeEmpty backboneTagsHis10-MBP-3CExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Antares
Plasmid#74279PurposeConstitutive expression of Antares in bacteriaDepositorInsertAntares
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn WT
Plasmid#36046DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-hAos1
Plasmid#53135PurposeE.coli expression of full length human Aos1 (SAE1) with Nterminal His-tagDepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET11a-SUMO2
Plasmid#53142PurposeE.coli expression of untagged mature human SUMO2 (amino acids 1-92)DepositorAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-D4H*-mCherry
Plasmid#134604Purposefluorescent cholesterol-binding probeDepositorInsertD4H*
TagsGST and mCherryExpressionBacterialMutationY415A and A463WAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBB541
Plasmid#27394DepositorAvailable SinceMarch 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP_PrA/G-H6
Plasmid#176077PurposeExpress His6 tagged MBP_PrA/G in Escherichia coliDepositorInsertMBP PrA/G-H6
TagsHis tagExpressionBacterialPromoterT7 PromoterAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFC332
Plasmid#87845PurposeAMA1 plasmid with Aspergillus optimized Cas9 and hph selection markerDepositorInsertsCas9
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA
Plasmid#216229PurposeS. aureus USA300 GyrA Codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX3X-RanBP2dFG
Plasmid#53120PurposeE.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activityDepositorInsertRanBP2 fragment (RANBP2 Human)
TagsGSTExpressionBacterialMutationfragment (amino acids 2553-2838)PromotertacAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato
Plasmid#180152PurposetdTomato cre reporter with Rosa26 recombineering homology armsDepositorInserttdTomato
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-G3
Plasmid#135521PurposeEngineered form of fibroblast growth factor 2 with improved thermostabilityDepositorInsertfibroblast growth factor 2 (FGF2 Human)
Tags6x His tag and Agg Thrombin TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTUM4
Plasmid#230905PurposePlasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coliDepositorInsertsExpressionBacterialAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PSMD10
Plasmid#31332DepositorInsertPSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-zeoCV
Plasmid#73890Purposefission yeast drug selection markerDepositorInsertzeoCV
ExpressionBacterial, Mammalian, and Y…PromoterCMVAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEM7068
Plasmid#214006PurposePrpsM driven expression of ssrA-tagged eGFP (ASV)DepositorInserteGFP-ASV
ExpressionBacterialPromoterPrpsMAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
spNW30
Plasmid#173485PurposeExpresses spNW30 in Bl21DepositorInsertspNW30
TagsHistagExpressionBacterialAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
AB-GFP
Plasmid#72203Purposescreen AB42 variants for aggregationDepositorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4
Plasmid#196071PurposeExpress wild type SG4 in E.coliDepositorInsertSG4
Tags3XFLAG and 6XHisExpressionBacterialPromoterT7Available SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pE-FLP
Plasmid#45978PurposeExpresses yeast Flp driven by the constitutive promoter pE from phage P2DepositorInsertflp
UseSynthetic BiologyExpressionBacterialMutationflp driven by the constitutive promoter pE from p…PromoterpE (from phage P2)Available SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mCherry
Plasmid#102629PurposeIn vitro expression of mCherry from the T7 promoterDepositorInsertmCherry
ExpressionBacterialPromoterT7Available SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-Ec1
Plasmid#138474PurposeEnables high-efficiency recombineering in E. coliDepositorInsertsCspRecT
mutL E32K
ExpressionBacterialMutationE32KAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCV020-tetQ
Plasmid#232826PurposeRandom transposon mutagenesis vector for Bacteroidales species - tetracyclineDepositorInserttetQ
ExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp15-mNeongreen
Plasmid#182389PurposeBacterial expression of Vesicle Nucleating peptide15-mNeongreen fusionDepositorInsertVNp15-mNeongreen-His
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2
Plasmid#190181PurposeFluorescent reporter vector to visualize all of cell cycle phases.DepositorInsertsExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLBH531_MBP-Cas14a1 expression
Plasmid#112500PurposeExpresses MBP fused to Cas14a1DepositorInsertCas14a1
UseCRISPRTags10xHis-MBPExpressionBacterialPromoterT7Available SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mTagBFP2
Plasmid#102638PurposeIn vitro expression of mTagBFP2 from the T7 promoterDepositorInsertmTagBFP2
ExpressionBacterialPromoterT7Available SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-mTurquoise2
Plasmid#108235PurposeBroad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.DepositorInsertPc promoter - mTurquoise2
ExpressionBacterialMutationcodon optimized for experession in P. fluorescensAvailable SinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3z/601
Plasmid#26656DepositorInsert
ExpressionBacterialAvailable SinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLEVI(408)-ColE-iLight-msfGFP
Plasmid#170268PurposeExpresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.DepositorInsertiLight
TagsmsfGFPExpressionBacterialMutationTo design iLight for expression in bacteria, a fu…PromoterpJ23116Available SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Taq DNA Ligase
Plasmid#178798PurposeInducible expression of Taq DNA Ligase in E. coliDepositorInsertTaq DNA Ligase
ExpressionBacterialAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28:GFP
Plasmid#60733PurposepET28 driving expression of GFP, biobrick part Bba_E0040DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPARA2
Plasmid#200417PurposeGolden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)DepositorArticleTypeEmpty backboneExpressionBacterialPromoterpBADAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
BirA in pET28a (w400-2)
Plasmid#26624DepositorInsertBirA
TagsHisExpressionBacterialAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOPARA1
Plasmid#200416PurposeGolden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)DepositorArticleTypeEmpty backboneExpressionBacterialPromoterpBADAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
NW30
Plasmid#133444PurposeCovalently circularized nanodisc NW30 for studying membrane proteins and viral entry.DepositorInsertNW30
ExpressionBacterialAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pTn Donor
Plasmid#236211PurposeArabinose-inducible Tn5 InducTn-seq plasmidDepositorInsertNone
ExpressionBacterialAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
Raf-1 GST RBD 1-149
Plasmid#13338DepositorAvailable SinceOct. 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AlkB
Plasmid#228218PurposeExpress wild type AlkB protein in E. coli BL21(DE3)DepositorInsertAlpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
TagsHis tagExpressionBacterialMutationcodon optimizedPromoterT7Available SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b RNase A
Plasmid#58903PurposeBacterial expression of bovine pancreatic ribonuclease ADepositorInsertRNase A (RNASE1 Bovine)
TagspelB sequenceExpressionBacterialMutationSee Depositor Comments belowPromoterT7Available SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-IPP1-His
Plasmid#118978PurposeBacterial expression plasmid for Inorganic pyrophosphataseDepositorInsertinorganic diphosphatase IPP1 (IPP1 Budding Yeast)
Tags6x-HisExpressionBacterialPromoterT7 promoterAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNR220
Plasmid#79541PurposeInput plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.DepositorInsertsTP901
BxbI
A118
TagsHis tag and LAA degradation tagExpressionBacterialAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKL2299A
Plasmid#222105PurposeA ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.DepositorInsertvirA
ExpressionBacterial and PlantPromotervirA promoterAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only