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Showing: 501 - 550 of 29690 results

  2. pET32a-Trx-His6-TEV_SacB

    Plasmid
    #164588
    Purpose
    Bacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.
    Depositor
    Ernst Schonbrunn
    Insert
    TEV-SacB
    Use
    Tags
    Trx-His6 followed by TEV site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    164588-plasmid-map-sequence-id-337962

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  5. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

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  6. pB025

    Plasmid
    #183092
    Purpose
    Expresses FnCas12a in Bacteroides and used for genome editing
    Depositor
    Lei Dai
    Article
    Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47
    Insert
    FnCas12a, gRNA, Promoter, HAB, TetR,
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1TDPGH023
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183092-plasmid-map-sequence-id-368147

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  7. mEGFP-pBAD

    Plasmid
    #54622
    Purpose
    Localization: Protein Expression Vector , Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    6xHis and mEGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54622-plasmid-map-sequence-id-257491

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  8. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid
    #218190
    Purpose
    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)
    Depositor
    Florence Pojer
    Insert
    nucA
    Use
    Tags
    His and NSP4 signal secretion peptide
    Expression
    Bacterial
    Mutation
    codon optimized ecoli residues - 22-266
    Promoter
    T7
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218190-plasmid-map-sequence-id-467313

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  11. bZiPro

    Plasmid
    #86846
    Purpose
    express NS2B-NS3 protease in E.coli
    Depositor
    Dahai Luo
    Article
    Zhang et al Science. 2016 Dec 8. pii: aai9309.
    Inserts
    NS3 1-177
    NS2B45-96
    Use
    Tags
    a hexahistidine tag and a thrombin cleavage site
    Expression
    Bacterial
    Mutation
    NS2B cofactor region (residues 45–96) and NS3 pro…
    Promoter
    T7
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86846-plasmid-map-sequence-id-172845

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  16. pET22b_PstS_1

    Plasmid
    #78198
    Purpose
    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A197C (please see depositors comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78198-plasmid-map-sequence-id-146048

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  21. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

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  22. FKBP8

    Plasmid
    #25609
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FKBP8 (FKBP8 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    FKBP-type PPIase Domain, residues 90-205
    Promoter
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25609-plasmid-map-sequence-id-454823

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  25. pTriEx4-Cdc42-2G

    Plasmid
    #68814
    Purpose
    FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)
    Depositor
    Olivier Pertz
    Article
    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10
    Insert
    Cdc42 activation reporter
    Use
    Tags
    His and Myc
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    CMV
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    68814-plasmid-map-sequence-id-120972

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  26. p2B-58

    Plasmid
    #177928
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TET4 (contains four rtTA binding sites)
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177928-plasmid-map-sequence-id-349996

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  28. pEVOL-pBpF

    Plasmid
    #31190
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.
    Depositor
    Peter Schultz
    Article
    Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99
    Insert
    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G, E107P, D158T, I159S
    Promoter
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31190-plasmid-map-sequence-id-418765

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  30. pET28-MBP-super TEV protease

    Plasmid
    #171782
    Purpose
    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    MBP-sTEV
    Use
    Tags
    Arg6 tag and Internal His6 tag
    Expression
    Bacterial
    Mutation
    No internal TEV cleavage site between the MBP and…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171782-plasmid-map-sequence-id-443047

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  32. RepX_pET28a(+)

    Plasmid
    #172199
    Purpose
    Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.
    Depositor
    Taekjip Ha
    Article
    Arslan et al Science. 2015 Apr 17;348(6232):344-7. do
    Insert
    RepX superhelicase
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    C18L, C43S, C167V, S400C, C612A
    Promoter
    Available since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    172199-plasmid-map-sequence-id-356977

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  34. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  39. pTO_CHMP4B_LAP

    Plasmid
    #101850
    Purpose
    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101850-plasmid-map-sequence-id-200248

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  40. p2B-56

    Plasmid
    #177925
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    MYO2
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177925-plasmid-map-sequence-id-349995

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  43. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

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  44. pNP1723

    Plasmid
    #222727
    Purpose
    sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)
    Depositor
    Patrick Hsu
    Article
    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:
    Type
    Empty backbone
    Use
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222727-plasmid-map-sequence-id-440095

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  47. pCPP5371

    Plasmid
    #128732
    Purpose
    Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tag
    Depositor
    Alan Collmer
    Article
    Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    128732-plasmid-map-sequence-id-252578

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Showing: 501 - 550 of 29690 results