31,059 results
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Plasmid#124931PurposeExpression of GFP-SZ2DepositorInsertGFP-SYNZIP2
TagsGFP and HisExpressionBacterialMutationSZ2 domainAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
NylCK_pET28a
Plasmid#215422PurposeExpression construct for NylCK gene, codon optimized for expression in E. coliDepositorInsertNylCK
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCis GFPuv-SS Himar1 A7
Plasmid#84686PurposeCan be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
KLHL12
Plasmid#38908PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-TfCut2-S121P-D174S-D204P
Plasmid#218695PurposeEncodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expressionDepositorInsertTfCut2
Tags10xHisExpressionBacterialMutationS121P-D174S-D204PAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEERM4 Cm
Plasmid#64026PurposePlasmid for integration into neutral site 2 of Synechocystis 6803DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPnrsBAvailable SinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMW363
Plasmid#238208PurposeExpresses TasR with an N-terminal Twin-StrepII tag, followed by 3xRNA1 TIGR array. AmpRDepositorInsertTaTasR
TagsTwinStrepIIExpressionBacterialAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMW364
Plasmid#238205PurposeExpresses untagged TasR from a PBAD promoter, followed by 3xRNA1 TIGR arrayDepositorInsertTaTasR
ExpressionBacterialAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMBEC2
Plasmid#187596PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
ExpressionBacterialPromotertrcAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8e
Plasmid#208319PurposeOverxpression MBP-TadA8eDepositorInsertTadA8e
ExpressionBacterialMutationmutations in TadA are described in the paperAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbE7a-RFP
Plasmid#35313DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAN3938
Plasmid#74697PurposeCircuit plasmid: 0xF6DepositorInsertCircuit 0xF6
ExpressionBacterialAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
WT C-His Ana GvpC in pET28a
Plasmid#85732PurposeExpresses His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (his-tagged)
TagsHis-tagExpressionBacterialMutationAdded an extra glycine after the methionine at th…PromoterT7Available SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMAL-PUM1
Plasmid#120384PurposeBacterial expression of MBP-Pumillio1 fusion proteinDepositorAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pHybr_r2a>mA-srt-his
Plasmid#124806PurposeHDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.DepositorInsertMurine IgA
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
Plasmid#83485PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A_R1048A_H1053A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477A, R1048A, H1053AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-hYAP2
Plasmid#17844DepositorAvailable SinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pE2n
Plasmid#17456Purposemodified pENTR vector for N-terminal 3x HA tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags3xHAExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBJ1
Plasmid#167130PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterFrr promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-X(4130)B1
Plasmid#168018PurposeExpresses X(4130)B1-6xHis in bacterial cellsDepositorInsertX(4130)B1-6xHis
TagsHisx6ExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEvoltac-EcW-TGA-h14
Plasmid#218764Purposeencodes EcWRS-h14 and EcW-tRNA-TGADepositorInsertEcWRS-h14
ExpressionBacterialMutationS8A, V144G, V146CPromotertacIAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
Plasmid#172503PurposeEncodes the DisCas7-11 protein only for bacterial recombinant protein expression for purificationDepositorInsertDisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
ExpressionBacterialAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-morange
Plasmid#78468PurposeBroad range bacterial fluorescence labelingDepositorInsertmOrange
ExpressionBacterialAvailable SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWT019a
Plasmid#107893PurposeW1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1DepositorInsertPTetO-sd2U-Cas9, PLacO-sgRNA1
ExpressionBacterialAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG025 (DUF4297 + HerA)
Plasmid#157903PurposeExpresses the DUF4297 + HerA defense systemDepositorInsertDUF4297 + HerA
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBb(B5)1k-GFPuv
Plasmid#106393PurposeIPTG-inducible trc promoter expressing gfpuv on BBR1-B5 originDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL18
Plasmid#183390PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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IVT-nCas9(H840A)
Plasmid#207455Purposein vitro transcription of mRNADepositorInsertH840A
ExpressionBacterialAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQLinkG2
Plasmid#13671DepositorInsertmulti cloning site
UseCo-expressionTagsGST, TEV, and attB1ExpressionBacterialAvailable SinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
SpyLigase
Plasmid#51722PurposePlasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!DepositorInsertSpyLigase
TagsHis6 TagExpressionBacterialPromoterT7Available SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB3-eGFP-6xHis
Plasmid#130983PurposeBacterial expression of mouse EB3-GFP-6xHis for protein purification.DepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-Lux
Plasmid#109383PurposeArabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coliDepositorInsertpARABAD-LUX operon
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.847
Plasmid#108524PurposePhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.DepositorInsertPhlFAM + PPhlF-YFP
ExpressionBacterialMutationPhlF: S50G S73I R95H A121V M137I N144D T155I R200CAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTCF7 HLA-B*07:02
Plasmid#180446PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*07:02
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_HA
Plasmid#184866PurposepSwap plasmid part with HA homology armsDepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI
Plasmid#115209PurposeExpresses PbuCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPbuCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP Asn10 TEV LIC cloning vector (1C)
Plasmid#29654DepositorTypeEmpty backboneTags10xAsn, 6xHis, MBP, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -