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31,059 results

Showing: 7001 - 7040 of 31059 results

  13. htpG1-GFP

    Plasmid
    #109387
    Purpose
    GFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    phtpG1-GFP
    Expression
    Bacterial
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109387-plasmid-map-sequence-id-210728

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  16. pSK-mPar1

    Plasmid
    #53227
    Purpose
    bacterial expression of mouse Par1
    Depositor
    Shaun Coughlin
    Insert
    Par1 (F2r Mouse)
    Expression
    Bacterial
    Available Since
    July 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pCR8/GW/TOPO PCGF2

    Plasmid
    #69791
    Purpose
    Gateway entry vector for PCGF2
    Depositor
    Gerard Cagney
    Insert
    PCGF2 (PCGF2 Human)
    Expression
    Bacterial
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pET-A3A(N57Q)-BE3

    Plasmid
    #158157
    Purpose
    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS
    Depositor
    Keith Joung
    Article
    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10
    Insert
    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    Tags
    6xHis tag on N-terminus
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    158157-plasmid-map-sequence-id-309498

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  21. pTRC-LIC

    Plasmid
    #62343
    Purpose
    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    tac promoter from pKK233-2
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62343-plasmid-map-sequence-id-102266

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  22. RHOA

    Plasmid
    #73231
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RHOA (RHOA Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00071:Ras 7-180
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73231-plasmid-map-sequence-id-136222

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  23. pAD-SPS6-Pman

    Plasmid
    #133906
    Purpose
    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
    Depositor
    Oscar Kuipers
    Article
    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57
    Insert
    sfGFP(SPS6)
    Use
    E.coli/bacillus spp. shuttle vector
    Expression
    Bacterial
    Promoter
    Pman
    Available Since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    133906-plasmid-map-sequence-id-261727

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  34. pET28GST-LIC

    Plasmid
    #26101
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6XHis, GST, and Thrombin CLeavage Site
    Expression
    Bacterial
    Available Since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26101-plasmid-map-sequence-id-142370

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  35. K980

    Plasmid
    #129762
    Purpose
    Expresses Drosophila Kinesin-1 full-length for microtubule gliding assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    His6
    Expression
    Bacterial
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129762-plasmid-map-sequence-id-263587

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  39. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

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  40. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

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Showing: 7001 - 7040 of 31059 results