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Plasmid#90314PurposemVirD2 expression plasmidDepositorInsertA. tumefaciens VirD2 from aa 1 to 204
UseTagsHis6x tagExpressionBacterialMutationPromoterT7/T5 IPTG inducibleAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i2
Plasmid#120883PurposeExpresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.DepositorInsertCas12i2
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQnl_Pcphy23
Plasmid#191354PurposeClostridium plasmid expressing NanoLuc reporter from moderate, constitutive promoterDepositorInsertPcphy23-NanoLuc
UseTagsExpressionBacterialMutationPromoterPcphy23Available sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-GB1 Leu5Met
Plasmid#128686PurposeExpresses GB1 Leu5Met mutantDepositorInsertspg
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJUMP18-cueR_O
Plasmid#127026PurposeBasic Part O- ORF; cueR; transcription factorDepositorInsertPart cueR_o
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTE1059
Plasmid#186628PurposeExpression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.DepositorInsertsscbA
scbB
scbC
UseSynthetic BiologyTags6xHis and 6xHis-thrombinExpressionBacterialMutationPromoterSynthetic promoter B10, Synthetic promoter B19, a…Available sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-EPL123
Plasmid#45361DepositorInsertAcrAB
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterLacUV5Available sinceJune 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pIB169
Plasmid#90192PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoterDepositorTypeEmpty backboneUseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCoofy6
Plasmid#43990DepositorTypeEmpty backboneUseTagsHis6-Sumo3ExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAR3040-spitz
Plasmid#38341DepositorInsertspitz (spi Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_E83K
Plasmid#109412PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationE83K repA mutation to increase copy numberPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-E1103G
Plasmid#10989DepositorInsertrpb1-E1103G (RPO21 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMutation 3308 A to G in the RPB1 ORF resulting in…PromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pColdI-GST-mGTSF1l
Plasmid#214817PurposeBacterial expression of GST-tagged mouse GTSF1lDepositorInsertGTSF1l (Gtsf1l Mouse)
UseTags6x His and GSTExpressionBacterialMutationPromoterCspAAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-FL-syt1
Plasmid#213628PurposeBacterial expression of SUMO-tagged rat FL-syt1DepositorInsertFL-syt1 (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pKCyGe2
Plasmid#167515PurposeBroad-Host range expression vector, RK2 and pUC origin, CymRAM repressor, PCymRC promoter controlling GmR, Kanamycin Resistance Marker.DepositorInsertCymRAM-GmR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87E/S129E
Plasmid#36064DepositorInsertalpha-synuclein S87E/S129E (SNCA Human)
UseTagsExpressionBacterialMutationS87E/S129EPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY7
Plasmid#21766DepositorInsertECFP -EYFP linked by flexible linker containing 7 SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EIF4E_IF4E
Plasmid#110010PurposeProtein expression and purification of EIF4E_IF4EDepositorInsertEIF4E_IF4E (EIF4E Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_4
Plasmid#60576Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_4
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-HpENR
Plasmid#12651DepositorInsertnoyl-acyl carrier protein reductase (fabI Helicobacter pylori)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LOV-ipaA
Plasmid#40236DepositorInsertLOV-ipaA
UseTags6xHisExpressionBacterialMutationJα helix region hybridized (AsLOV2 aa 537-546/ipa…PromoterT7Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTn7·xylS
Plasmid#122591PurposePlasmid for genomic integraction of the xylS regulator into the Tn7 insertion siteDepositorInsertxylS
UseSynthetic Biology; Tn7 genomic integrationTagsExpressionBacterialMutationPromoterPmAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-DsRed-Express2
Plasmid#111260Purposemini-Tn7 vector for insertion of DsRed-Express2 at bacterial attTn7 siteDepositorInsertDsRed-Express2
UseTagsExpressionBacterialMutationPromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a.TM0857
Plasmid#11419DepositorInsertflavin binding protein (TM0857 T. maritima)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2-Gal11p
Plasmid#127481PurposePositive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interactionDepositorInsertLGF2-Gal11p (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA
Plasmid#127464PurposeAVA-seq plasmid with duel BstXI sitesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2(fs)-Gal11p(fs)
Plasmid#127483PurposeNegative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interactionDepositorInsertLGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationOne nucleotide inserted between lambda CI and LGF…PromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2(fs)
Plasmid#127482PurposeNegative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interactionDepositorInsertGal11p-LGF2(frame shifted) (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationLGF2 is frame shifted by the insert of one nucleo…PromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBORF-DBD
Plasmid#127463PurposeORF filtering plasmid (lambda cI associated plasmid for pAVA)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Orf9b
Plasmid#156485PurposeBacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tagDepositorInsertOrf9b
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Delta DED FLIP-L
Plasmid#13790DepositorInsertDELTA DED FLIP LONG (CFLAR Human)
UseTags8 x His tagExpressionBacterialMutationDelta DED removes first 186 residuesPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX C_K nsp1 CoV2
Plasmid#175516PurposeExpresses C_K for bioconjugation on nsp1DepositorInsertC_K nsp1 CoV2
UseTagsGST tagExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Sh3bp2-SH2
Plasmid#46484DepositorInsertSh3bp2 (SH3BP2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 450-561PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2
Plasmid#191751PurposeMicrotubule marker together with nuclear envelope marker driven by bidirectional promoterDepositorUseTagsGdp1 promoter, ToxA promoter, iRFP670, and mTagBF…ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKS13
Plasmid#24627DepositorInsertpdc, adhB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCVD438
Plasmid#63759Purposeeae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.DepositorInserteae
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGST-PPARdelta
Plasmid#16548DepositorInsertPPAR delta (PPARD Human)
UseTagsGSTExpressionBacterialMutationN-terminal DNA-binding domain: aa 1-249PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPDS1-mEGFP-IRES-puro2b
Plasmid#26464DepositorInsertpPDS1 (PTTG1 Human)
UseTagsmEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
LaM8-GG15
Plasmid#159593PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at GG15
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC572
Plasmid#127973PurposeAMA1 plasmid with pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertpyrG
UseFungal expressionTagsExpressionBacterialMutationPromoternative promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-2_dCrk
Plasmid#131146PurposeExpresses GST::dCrk (based on crk isoform RA).DepositorInsertCrk (Crk Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTB-4
Plasmid#22820DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET15-MTFmt
Plasmid#31174DepositorInsertMature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)
UseTagsHis and thrombin cleavageExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSS2 (hS100B)
Plasmid#26774DepositorInsertS100B (S100B Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SERPINA5_Serpin
Plasmid#110034PurposeProtein expression and purification of SERPINA5_SerpinDepositorInsertSERPINA5_Serpin (SERPINA5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a H759A
Plasmid#113518PurposeExpresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processingDepositorInsertLbCas12a
UseTagsMBPExpressionBacterialMutationH759APromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRF-BM3R1
Plasmid#49358DepositorInsertBM3R1 repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only