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Showing: 11101 - 11150 of 29646 results

  3. ZP16 Wnt-1

    Plasmid
    #16925
    Depositor
    Randall Moon
    Insert
    wnt-1 (wnt1 Zebrafish)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only

  7. pET-24a-STE2

    Plasmid
    #133430
    Purpose
    Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Both native Cys (C59S/C252A) have been mutated. S…
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133430-plasmid-map-sequence-id-267723

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  10. pYTK084

    Plasmid
    #65191
    Purpose
    Encodes KanR-ColE1 as a Type 8 part to be used in the Dueber YTK system
    Depositor
    John Dueber
    Article
    Lee et al ACS Synth Biol. 2015 Apr 14.
    Insert
    KanR-ColE1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65191-plasmid-map-sequence-id-110244

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  12. pGBW-m4046933

    Plasmid
    #145592
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145592-plasmid-map-sequence-id-292573

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  20. pNIC28-Bsa4-TF

    Plasmid
    #61689
    Purpose
    E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    HIS and Trigger Factor
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. pOP5GT

    Plasmid
    #112611
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-GST and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112611-plasmid-map-sequence-id-220247

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  33. 6xHis-MBP-huLb5Cas12a

    Plasmid
    #174684
    Purpose
    Expresses 6xHis-MBP tagged Lb5Cas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huLb5Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174684-plasmid-map-sequence-id-346193

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  42. SPR

    Plasmid
    #39161
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (SPR Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  45. pDCAF3-IS5

    Plasmid
    #65220
    Purpose
    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.
    Depositor
    Jeffrey Barrick
    Article
    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.
    Inserts
    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed start codon from gtg to atg and Changed s…
    Promoter
    BBa_J23100
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    65220-plasmid-map-sequence-id-134450

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  49. pGBW-m4046921

    Plasmid
    #145755
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145755-plasmid-map-sequence-id-292520

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  50. MLYCD

    Plasmid
    #39081
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MLYCD (MLYCD Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Surface mutant for crystallisation E19A, K20A, E2…
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 11101 - 11150 of 29646 results