29,693 results
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SAE2-mGarnet
Plasmid#175240PurposeBacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimerDepositorInsertUBA2 (UBA2 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT316
Plasmid#8608DepositorInsertbarstar
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS_nanorod_105b
Plasmid#120347Purposephagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)DepositorInsertStaple sequences for a DNA origami nanorod
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-mOVA
Plasmid#25099DepositorInsertmembrane bound ovalbumin
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7FTrp)RS
Plasmid#177310PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-E2Crimson
Plasmid#78473PurposeBroad range bacterial fluorescence labelingDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-EsCas13d
Plasmid#108303PurposeExpresses E. coli codon optimized EsCas13d in the pET28a backbone.DepositorInsertEsCas13d
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b Tau Cys
Plasmid#108867PurposeExpresses human Tau with a mutated cysteineDepositorInsertTau (MAPT Human)
UseTagsExpressionBacterialMutationI260C C291A C322A to allow maleimide dye labellingPromoterT7Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-NRG1B1
Plasmid#120287PurposeProduce human recombinant NRG1 in DE3 cellsDepositorInsertNRG1-beta 1
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPO3
Plasmid#209977PurposeContains Level 0 Part: replication origin (pAMBeta1) for the construction of Level 1 plasmidsDepositorInsertreplication origin (pAMBeta1)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p15TV-L
Plasmid#26093PurposeSGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.DepositorTypeEmpty backboneUseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceSept. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF3
Plasmid#228777PurposeProtein expression plasmid for YTH domain of YTHDF3 in E.Coli.DepositorInsertYTH domain of YTHDF3 (YTHDF3 Human)
UseTags6*HisExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF2
Plasmid#228776PurposeProtein expression plasmid for YTH domain of YTHDF2 in E.Coli.DepositorInsertYTH domain of YTHDF2 (YTHDF2 Human)
UseTags6*HisExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBADZ-HisCre
Plasmid#111187PurposeHelper plasmid containing Cre-recombinase gene, which is expressed in DH10MultiBac cellsDepositorInsertCre recombinase
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ELP[V]60
Plasmid#219406Purpose(VPGVG)60DepositorInsertELP[V]60
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-UbE2D3
Plasmid#186805PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.DepositorInsertHis-Tev-UbE2D3 (UBE2D3 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYHY1
Plasmid#215926PurposeE. coli-Bacteroides shuttle vector.DepositorTypeEmpty backboneUseSynthetic Biology; E. coli-bacteroides shuttle ve…TagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVSL10
Plasmid#201182PurposeBst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavableDepositorInsertBst polymerase large fragment (BF) F710Y/D598A
UseTagsHRV 3C protease cleavage site and His6ExpressionBacterialMutationF710Y D598APromoterT5-lacOAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSGKP-spe
Plasmid#117234PurposeA sgRNA expression plasmid for genome editing in Klebsiella PneumoniaeDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA7CR_2.0
Plasmid#73950PurposepMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recXDepositorInsertscas9
lambda beta
dam
recX
UseTagsExpressionBacterialMutationPromoterpAra and pTetAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-nanoHER2-SpyTag003
Plasmid#216312PurposeExpresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.DepositorInsertnanoHER2-SpyTag003
UseTagsHis6 tag and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TadCBE-Cas9NG
Plasmid#225093PurposePlasmid for bacterial purification of codon-optimized TadCBE-Cas9NGDepositorInsertTadCBE-Cas9NG
UseCRISPRTags8xHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTOX4
Plasmid#127449PurposeEncodes YhaV toxin and amilCP chromoprotein. CAM-RDepositorInsertsyhaV toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pComb3XTT
Plasmid#63891PurposeBackbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)DepositorInsertTT Fab
UsePhage displayTags6xHis, HA tag, and geneIIIExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK cyclin T1 (P#468)
Plasmid#14607DepositorInsertcyclin T1 (CCNT1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBR322-PVCpnf18-21
Plasmid#198273PurposeContains only regulatory genes; for generating empty PVCsDepositorInsertPVCpnf18-21
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET259-pUC57 24xMS2V6
Plasmid#104391PurposeMS2V6 cloningDepositorInsert24xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET N6his GFP1-9
Plasmid#182240PurposeTripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.DepositorInsertGFP1-9
UseTags6HISExpressionBacterialMutationPromoterT7Available sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis T5 Exonuclease
Plasmid#206209PurposeInducible expression of T5 exonucleaseDepositorInsertT5 exonuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB46 (CK2beta)
Plasmid#27085DepositorInsertCK2beta (CSNK2B Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET15-MHL
Plasmid#26092PurposeSGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.DepositorTypeEmpty backboneUseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceSept. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a Halotag-CNA35
Plasmid#131128PurposeHalotag-CNA35 peptide for Collagen labellingDepositorArticleInsertHalotag-CNA35
UseTagsHalotagExpressionBacterialMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTOX11
Plasmid#127456PurposeEncodes MqsR toxin. Gent-RDepositorInsertmqsR toxin
UseTagsExpressionBacterialMutationPromoterpRhaAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMyC-kan
Plasmid#84692PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterAcetamidaseAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPET-PKR/PPase
Plasmid#42934DepositorInsertsUseTagsExpressionBacterialMutationPromoterT7Available sinceApril 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNPTS138 Cm
Plasmid#41891DepositorTypeEmpty backboneUseBacterial allelic exchange vector with sacbTagsExpressionBacterialMutationPromoterAvailable sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMAZ-SK
Plasmid#73962Purposeself-killing gRNA plasmid pMAZ-SKDepositorInsertgRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLS.402
Plasmid#187202PurposeExpresses retron Eco1 RTDepositorInsertRetron Eco1 Reverse Transcriptase
UseSynthetic BiologyTagsExpressionBacterialMutationPromotermphRAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEND-418_Cacnes_LysA
Plasmid#225607PurposeSuicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sitesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top2A-5X Gly-Venus-T2A-Hygro
Plasmid#211115PurposeFor tagging the C-term of Top2A with VenusDepositorInsertsUseCRISPRTagsExpressionBacterial and MammalianMutationPromotern/aAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
KTK_002
Plasmid#180527PurposeEntry vector containing J23104 promoterDepositorInsertJ23104 promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTOX10
Plasmid#127455PurposeEncodes YhaV toxin. Gent-RDepositorInsertyhaV toxin
UseTagsExpressionBacterialMutationPromoterpRhaAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-E2-25K
Plasmid#63572PurposeExpression of human Ubiquitin conjugating enzyme E2-25KDepositorInsertUBE2-25K (UBA7 Human)
UseTagsGSTExpressionBacterialMutationIsoform 1 (200 resiudes)PromotertacAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24b-BG1.3
Plasmid#199437Purposeexpresses BAmGreen1.3 in bacterial cellsDepositorInsertBAMGreen1.3
UseTagshis tagExpressionBacterialMutationPromoterT7Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
R701-X65-527: His6-tev-Hs.CALM1(2-148)
Plasmid#159693PurposeE. coli protein expression of His6-tev-Hs.CALM1(2-148)DepositorInsertHis6-tev-Hs.CALM1(2-148) (CALM1 Human)
UseTagsHis6-tevExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b RNase A
Plasmid#58903PurposeBacterial expression of bovine pancreatic ribonuclease ADepositorInsertRNase A (RNASE1 Bovine)
UseTagspelB sequenceExpressionBacterialMutationSee Depositor Comments belowPromoterT7Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
spNW100
Plasmid#173488PurposeExpresses spNW100 in Bl21DepositorInsertspNW100
UseTagsHistagExpressionBacterialMutationP1049L- please see depositor commentsPromoterT7Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only
