31,059 results
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Plasmid#200967Purposeinduction of phase-separated condensate mediated by FUSN-IDRDepositorInsertFUSN
UseLentiviralExpressionBacterial and MammalianAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-TRXtag
Plasmid#11516DepositorInsertNone - thioredoxin is vector encoded (trxA )
TagsHis, His (out of frame), and TrxExpressionBacterialMutationvector modified; removed N-terminal His tag by di…Available SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCCM’
Plasmid#162709PurposepCCM reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationp15A origin replaced with high-copy colE1 originPromoterPLtet0-1 promoterAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR83
Plasmid#90211PurposeExpresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoterDepositorAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2-Ct
Plasmid#79158PurposeExpresses the Ct biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Ct
ExpressionBacterialPromoterT7Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT
Plasmid#154271PurposeBacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationbacterial codon optimizedAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEVAT7K1A
Plasmid#58302PurposeGeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as cargo.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBG100-TCPTP
Plasmid#33365DepositorAvailable SinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX-EZH2
Plasmid#28060DepositorAvailable SinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET21-GFP-AURKA-mCherry
Plasmid#99876PurposeExpression of GFP-AURKA-mCherry for protein purification in bacteriaDepositorAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSP3545-dCas9Str
Plasmid#153516PurposeExpresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backboneDepositorInsertdCas9Str
ExpressionBacterialPromoternisAAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBZS-YP_009702000
Plasmid#198379PurposeExpress YP_009702000 in bacterial cells.DepositorInsertYP_009702000 (CVA-1_913R )
ExpressionBacterialAvailable SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
WIW_Scaffold2
Plasmid#205574PurposeNanobody that inhibits seeding of tau fibrilsDepositorInsertWIW_Scaffold2
Tags6X HisExpressionBacterialAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_48
Plasmid#109246PurposepSC101 var6 BbsI cloning vectorDepositorInsertsfGFP
ExpressionBacterialPromoterPJ23105Available SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
CpxR-GFP
Plasmid#220150PurposeTo track the natural CpxR promoter's transcriptional dynamicsDepositorInsertCpxR promoter only
ExpressionBacterialAvailable SinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK172-6xHis-0N4R-P301S-tau-HiBiT
Plasmid#187835PurposeExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.DepositorInsert6XHis-0N4R-P301S-tau-HiBiT
Tags6xHistidine and HiBiTExpressionBacterialPromoterT7Available SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
Tags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐hsRNASEH2BCA
Plasmid#108692PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzymeDepositorTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon a…PromotertacAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKm
Plasmid#170331Purposebackbone vector for clone constructionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-mCherry-6xHis
Plasmid#130984PurposeBacterial expression of mouse EB1-mCherry-6xHis for protein purification.DepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNCS mStrawberry
Plasmid#91768PurposeBacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)DepositorInsertmonomeric Strawberry
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGST1-TOM1-VHS
Plasmid#44423DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_3RZY
Plasmid#234156PurposeHeterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
R6K_tac_dGFPmut3_ChInt
Plasmid#187388Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)DepositorInsertdGFPmut3_AAV
TagsAAVExpressionBacterialMutationC-terminal AAV tailAvailable SinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pAOX1
Plasmid#98514PurposeBB1 with insert: promoter PAOX1DepositorInsertAOX1 promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL8B
Plasmid#67383PurposeExpression of native hArl8b in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
hFerritinL
Plasmid#122653PurposeExpression of hFerritinL in bacterial cellsDepositorInsertlight chain ferritin, human (FTL Human)
ExpressionBacterialAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-hTBP
Plasmid#171073PurposeExpresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBPDepositorAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRF-SrpR
Plasmid#49372DepositorInsertSrpR repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUC01
Plasmid#177204Purposechromogenic reporter for gene knockout in StreptomycesDepositorInsertSaindC
UseSynthetic BiologyTagsNONEExpressionBacterialMutationNONEPromoterTGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…Available SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_8
Plasmid#60579Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_8
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
AcpP pet29a C-terminal His Tag
Plasmid#75016PurposeExpresses the acyl carrier protein (ACP) from the E. coli fatty acid synthaseDepositorAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Citron1
Plasmid#134300PurposeBacterial expression of direct-response citrate sensorDepositorInsertCitron1
Tags6-His tagExpressionBacterialPromoterpBADAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30-HPF1-His-Sumo-Flag
Plasmid#111577PurposeExpresses full length HPF1 in E. coliDepositorAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-SNAP
Plasmid#38012DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
TagsSNAP26mExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRE132
Plasmid#118486Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
ExpressionBacterialAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crGE
Plasmid#172927PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
TagsHisExpressionBacterialPromoterT7Available SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEx-4T-1-YTHDF1
Plasmid#70087PurposeEncode YTHDF1 with N-terminal GST tag for E. coli expressionDepositorInsertYTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)
TagsGSTExpressionBacterialPromotertacAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-GFP-HiBit
Plasmid#170989PurposeFor bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-IL2 C125S
Plasmid#104348PurposeExpresses human IL-2 (C125S)DepositorInsertHuman IL-2 (C125S) (IL2 Human)
TagsMRGSHHHHHGS-tagExpressionBacterialMutationC125S mutation (TGT -> TCC)PromoterT5Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+Paxillin-mKate
Plasmid#105974Purposein vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesionsDepositorInsertPaxillin
TagsmKateExpressionBacterialAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
DLG1_PDZ_3
Plasmid#103917PurposeProtein expression and purification of DLG1 PDZ3 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#908
Plasmid#110914PurposeExpresses the plant proteins TIR1 (necessary for Auxin-Inducible Degradation) pan-neuronally in C. elegansDepositorInsertsrab-3 promoter
TIR1 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
ExpressionBacterialAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDB180
Plasmid#80677Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged proteinDepositorTypeEmpty backboneTagsOsmY-10His-Smt3ExpressionBacterialPromoterT7Available SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)
Plasmid#177850PurposeBacterial Expression of AMPKDepositorInsertAMPK (a1b2g1)
TagsMBPExpressionBacterialAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAurora
Plasmid#190158PurposeRegulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.DepositorTypeEmpty backboneTagsHis6 tagExpressionBacterialAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG79-attL1-pegRNA-attL2
Plasmid#213050PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBA620
Plasmid#186240PurposeLbuCas13a Negative Control (RFP targeting)DepositorInsertpTet-LbuCas13a + crRNA negative control
ExpressionBacterialAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTagMBP
Plasmid#35050DepositorInsertSpyTagMBP
TagsHis6ExpressionBacterialMutationSimple peptide tag added to the N-terminus of MBP…PromoterT7 promoterAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only