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Bacterial Expression

29,712 results

Showing: 2851 - 2900 of 29712 results

  1. pDUAL_tacI

    Plasmid
    #155170
    Purpose
    BaSP and CuCBP co-expression: tacI promoter controls BaSP expression
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    155170-plasmid-map-sequence-id-311666

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  3. M13cp-dg2 hp

    Plasmid
    #218094
    Purpose
    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pII
    Depositor
    Kevin Esvelt
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218094-plasmid-map-sequence-id-437754

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  4. pBJG1 T7-8xHis-OGT K842M

    Plasmid
    #154272
    Purpose
    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Lysine 842 changed to Methionine- bacterial codon…
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154272-plasmid-map-sequence-id-304833

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  5. pYFP

    Plasmid
    #175592
    Purpose
    Tet inducible, KAN Resistant, mVenus gene on a low copy backbone. Used as a negative control to the pRelA_YFP plasmid.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    mVenus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    An improved monomeric YFP. See doi: 10.1021/bi051…
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    175592-plasmid-map-sequence-id-348320

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  10. p5E-2.3k-fabp2

    Plasmid
    #84927
    Purpose
    –2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vector
    Depositor
    John Rawls
    Insert
    –2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
    Use
    Tags
    NO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    84927-plasmid-map-sequence-id-254043

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  11. pCR2.1-InC

    Plasmid
    #83959
    Purpose
    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.
    Depositor
    Kai-Shun Chen
    Insert
    Internal Control sequence
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83959-plasmid-map-sequence-id-160468

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  12. pET28a-His6-Ran(Q69L)

    Plasmid
    #171563
    Purpose
    Encodes human Ran protein
    Depositor
    Yan Zeng
    Insert
    Ran (RAN Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171563-plasmid-map-sequence-id-337921

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  18. pRedCmOcr

    Plasmid
    #176226
    Purpose
    A helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    Lambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaB and PL
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176226-plasmid-map-sequence-id-347002

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  19. pCCM’

    Plasmid
    #162709
    Purpose
    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    p15A origin replaced with high-copy colE1 origin
    Promoter
    PLtet0-1 promoter
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162709-plasmid-map-sequence-id-341131

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  23. mOrange-pBAD

    Plasmid
    #54751
    Purpose
    Localization: Protein Expression Vector , Excitation: 548, Emission: 562
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    6xHis and mOrange
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    54751-plasmid-map-sequence-id-453673

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  25. ECHS1

    Plasmid
    #38845
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ECHS1 (Echs1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. METTL1

    Plasmid
    #25264
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    METTL1_07 (METTL1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. UBC80

    Plasmid
    #25267
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc80.229.304 (TSG101 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pKD3_mCherry

    Plasmid
    #187385
    Purpose
    To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT
    Depositor
    Roi Avraham
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187385-plasmid-map-sequence-id-371123

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  38. t1-435

    Plasmid
    #166578
    Purpose
    For expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11
    Insert
    Tln1 Head (1-435) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-435 only
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166578-plasmid-map-sequence-id-330255

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  40. Capping Protein

    Plasmid
    #89950
    Purpose
    F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.
    Depositor
    Antoine Jégou, Guillaume Romet-Lemonne
    Article
    Shekhar et al Nat Commun. 2015 Nov 13;6:8730. doi: 10.
    Inserts
    F-actin-capping protein subunit alpha-1 (Capza1 Mouse)
    F-actin-capping protein subunit beta (Capzb Mouse)
    Use
    Tags
    6*His
    Expression
    Bacterial
    Mutation
    V222I
    Promoter
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    89950-plasmid-map-sequence-id-183281

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  43. pNB22

    Plasmid
    #103059
    Purpose
    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpAp
    Depositor
    Dirk Bumann
    Article
    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-
    Insert
    GFP-OVA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Promoter
    hmpAp
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    103059-plasmid-map-sequence-id-199053

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  44. pOPINB-HOIL-1 full-length (1–510)

    Plasmid
    #193858
    Purpose
    Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:
    Insert
    HOIL-1 (RBCK1 Human)
    Use
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    Codon optimised for expression in E. coli
    Promoter
    T7
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    193858-plasmid-map-sequence-id-379617

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  46. pMV306DIG13+FFlucRT

    Plasmid
    #49998
    Purpose
    Integrase removed to increase stability of the plasmid in mycobacteria
    Depositor
    Brian Robertson
    Article
    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2
    Insert
    G13 promoter + Firefly luciferase Red Thermostable
    Use
    Mycobacteria integrating vector
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for Mycobacterium tuberculosis wi…
    Promoter
    G13
    Available since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    49998-plasmid-map-sequence-id-74812

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  48. pCDF-mcherry1

    Plasmid
    #87144
    Purpose
    Constitutive E. coli mcherry Expression
    Depositor
    Michael Lynch
    Insert
    mcherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive proD
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87144-plasmid-map-sequence-id-176832

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Showing: 2851 - 2900 of 29712 results