31,059 results

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pMR-A-3-Sasg

Plasmid

#214349

Purpose

Expresses T7-RNAP, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

T7-RNAP

Expression

Bacterial

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_HECT

Plasmid

#187719

Purpose

Express GST-tagged NEDD4L HECT domain in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 594-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-593

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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MP6-SlugABEN-738

Plasmid

#214348

Purpose

Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE

Expression

Bacterial

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBT120a17-sg-del

Plasmid

#214347

Purpose

Expresses pIII

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

gIII-xLuxAB

Expression

Bacterial

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-151

Plasmid

#118565

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAGD248

Plasmid

#219498

Purpose

Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K

Expression

Bacterial

Promoter

pBAD

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pGTM_COV2_NSP5_004_SUMO

Plasmid

#190062

Purpose

Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

Depositor

Gaetano Montelione

Article

Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

Insert

3CLpro (ORF1ab SARS-CoV-2)

Tags

6X-His-Sumo

Expression

Bacterial

Promoter

T7 lac

Available Since

June 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVSV eGFP RABV-G

Plasmid

#31833

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Expression

Bacterial

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5Knob

Plasmid

#198289

Purpose

Produces PVCs retargeted with knob domain from hAd5 virus

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5Knob

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBK- Ma PylRS WT

Plasmid

#197573

Purpose

Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Insert

M. alvus PylRS WT

Expression

Bacterial

Promoter

GlnS (constitutive)

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1_D73C

Plasmid

#39328

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E3D1_D73C (APOA1 synthetic gene)

Tags

6xHis

Expression

Bacterial

Mutation

cys mutant of MSP1E3D1

Promoter

T7

Available Since

Sept. 11, 2012

Availability

Academic Institutions and Nonprofits only
pMRE-Tn7-145

Plasmid

#118561

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Zeo-gfpmut3

Plasmid

#65037

Purpose

ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

gfpmut3

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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Lac-I-SceI-Tet

Plasmid

#17655

Depositor

Tom Misteli

Article

Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

Insert

lac I-SceI tet

Expression

Bacterial

Available Since

May 30, 2008

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-pgaC

Plasmid

#154919

Purpose

aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting pgaC

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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LgBiT-ST

Plasmid

#207633

Purpose

A plasmid encoding LgBiT fused to SpyTag

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

LgBiT-SpyTag

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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ERG_pet28a

Plasmid

#131642

Purpose

Bacterial expression of ETS gene ERG

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ERG (ERG Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD/His-miRFP670nano3

Plasmid

#184663

Purpose

Monomeric near-infrared fluorescent protein miRFP670nano3 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3

Tags

His

Expression

Bacterial

Promoter

araC

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pScaf-5544.1

Plasmid

#111405

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v1)

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-GH'

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET16b-PCNA

Plasmid

#134898

Purpose

Bacterial expression and purification of human PCNA

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

PCNA (PCNA Human)

Tags

untagged

Expression

Bacterial

Mutation

fully synthetic, codon optimized for bacterial ex…

Promoter

T7

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDB6 (CK2alpha')

Plasmid

#27084

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' (CSNK2A2 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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mEos3-Rac1

Plasmid

#92039

Purpose

Expresses mEos3-Rac1 fusion protein

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCold-TF-hRubicon

Plasmid

#64147

Purpose

For protein expression in E. coli

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

Rubicon (RUBCN Human)

Tags

6xHis-tag and Trigger Factor (TF)

Expression

Bacterial

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
mEos3-dnRac1

Plasmid

#92040

Purpose

Expresses mEos3 fusion with dnRac1 (T17N).

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

T17N (dominant negative)

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_PDCD6_EF-hand

Plasmid

#110009

Purpose

Protein expression and purification of PDCD6_EF-hand

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PDCD6_EF-hand (PDCD6 Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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RARS_pNIC-Bio3

Plasmid

#153055

Purpose

Expression construct for producing Avi tagged Arginyl-tRNA synthetase

Depositor

Susanne Gräslund

Insert

RARS (RARS1 Human)

Tags

Avi and His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-WspR

Plasmid

#79162

Purpose

Expresses WspR WT under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pQE60-RRF-His

Plasmid

#124132

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Ribosome recycling factor (frr Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET-15b-UbcH5b/2419

Plasmid

#11475

Depositor

Allan Weissman

Article

Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

Insert

UbcH5b (UBE2D2 Human)

Expression

Bacterial

Mutation

n/a

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pASK-mSAN1-Streptag

Plasmid

#117165

Purpose

Expresses Strep-tagged murine SAN1 nuclease in bacteria

Depositor

Ian Macara

Article

Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

Insert

mSAN1 (Fam120b Mouse)

Tags

Streptag

Expression

Bacterial

Promoter

tetR/tetA

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAR17_StrepI_hFASh_H8_pET22b

Plasmid

#122846

Purpose

Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

Depositor

Martin Grininger

Article

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

human FASN/ human fatty acid synthase (FASN Human)

Tags

His8 tag and Twin-Strep tag

Expression

Bacterial

Mutation

wildtype

Promoter

T7 promoter

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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mEos3-caRac1

Plasmid

#92041

Purpose

Expresses mEos3 fusion with caRac1 (Q61L)

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L (constitutively active)

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-3xMBT

Plasmid

#46987

Purpose

Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli

Depositor

Or Gozani

Article

Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

Insert

Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)

Tags

GST and PreScission cleavage site

Expression

Bacterial

Mutation

contains amino acids 190–530 of accession NP_0562…

Promoter

tac

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2A

Plasmid

#15780

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2A (UBE2A Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pET32a-TRXtag

Plasmid

#11516

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

None - thioredoxin is vector encoded (trxA )

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

vector modified; removed N-terminal His tag by di…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pET11d-RAN

Plasmid

#108919

Purpose

Expresses human RAN

Depositor

Larry Gerace

Article

Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

Insert

RAN (RAN Human)

Expression

Bacterial

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-EZH2

Plasmid

#28060

Depositor

Mien-Chie Hung

Article

Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

Insert

EZH2 (EZH2 Human)

Tags

GST

Expression

Bacterial

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
AFDN_PDZ_1

Plasmid

#103938

Purpose

Protein expression and purification of TRITHORAX PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

AFDN (AFDN Human)

Tags

GST

Expression

Bacterial

Mutation

contains AA983-1110 of AFDN

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Annexin A7-TEV-TwinStrep

Plasmid

#198635

Purpose

Annexin A7 fibrillization and lipid binding

Depositor

Lalit Deshmukh

Article

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 (ANXA7 Human)

Tags

TEV-GS-Dual Strep

Expression

Bacterial

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbS8a-RFP

Plasmid

#35274

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

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