4,958 results
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Plasmid#111547PurposeOptogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p(bhsp68-Cas9)
Plasmid#65959PurposeTribolium basal heat shock promoter driving Cas9DepositorInsertCas9
UseCRISPRTagsnuclear localization sequenceExpressionInsectPromoterbhspAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc-y1sgRNA-Cas9
Plasmid#49331PurposeExpresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertsUseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-Cas9)
Plasmid#194768PurposeTo insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.DepositorInsertT2A-Cas9
UseCRISPRExpressionInsectAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pE3n
Plasmid#17457Purposemodified pENTR vector for N-terminal 6x MYC tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags6xMycExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDrBB2
Plasmid#18946DepositorInserthygromycin gene driven by the copia promoter, phiC31 attB
ExpressionInsectAvailable SinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pQUASp-RedStinger (nlsRFP)
Plasmid#46165PurposeExpression of RedStinger in insectDepositorTypeEmpty backboneExpressionInsectAvailable SinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTa_HCK
Plasmid#224245PurposeExpression of human HCK Kinase in insect cells (SF9 cells)DepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rabx6 WT
Plasmid#53479DepositorAvailable SinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUWR-Cad-Venus
Plasmid#58328PurposeDestination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genomeDepositorInsertCad-Venus
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_SNAP
Plasmid#82716PurposeExpresses Myosin VI HMM with a C-terminal SNAP tag.DepositorInsertMyosin VI
Tags6xHis Tag, FLAG purification tag, and SNAP tagExpressionBacterial and InsectPromoterT7 PromoterAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal80-3xP3-RFP
Plasmid#127562PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal80
UseCRISPRExpressionInsectAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
p(U6b-BsaI-gRNA)
Plasmid#65956PurposeTribolium U6b promoter with BsaI cloning site to insert guide RNA sequenceDepositorTypeEmpty backboneExpressionInsectPromoterU6Available SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
IL2 pAcGP67a
Plasmid#41806DepositorAvailable SinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3XP3.RFP-tan
Plasmid#83474PurposeRFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.DepositorInsert3XP3.RFP-tan
UseCRISPRExpressionBacterial and InsectPromoter3XP3 eye-expression PromoterAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINTRX
Plasmid#41116DepositorTypeEmpty backboneExpressionBacterial, Insect, and Mamm…Available SinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR
Plasmid#199197PurposeInsert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-split-intein-Gal4[1-20]-N-int-BlastR
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R
Plasmid#199198PurposeInsert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-split-intein-Gal4[21-881]-C-int-G418R
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Myc-BirA-G3-ER
Plasmid#173815PurposePromiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage siteDepositorInsertMyc-BirA-G3-ER
TagsMycExpressionInsectPromoterUASAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINTF
Plasmid#41114DepositorTypeEmpty backboneExpressionBacterial, Insect, and Mamm…Available SinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual
Plasmid#216377PurposeBaculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)DepositorInsertLDL receptor related protein 6 (LRP6 Human)
UseBaculovirusTags9xHis and HA signal sequence-FLAG-3C cleavage sit…ExpressionInsectPromoterPolyhedrin (LRP6)/p10 (MESD)Available SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1-T17N
Plasmid#22017PurposeExpression of photoactivatable Rac1 (dominant-negative mutant)DepositorInsertPA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
Tags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutation T17N.PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-hsp70
Plasmid#71500PurposeflySTARR-seq screening vector with hsp70 core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC154
Plasmid#118902Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
TagsTwinStrep, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQUASp-mCD8GFP
Plasmid#46163PurposeExpression of murine CD8GFP in insectsDepositorInsertmCD8GFP
ExpressionInsectAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
IMPT-2070
Plasmid#172092PurposepFastBac1 vector with GP64 promoter replacing polH promoter expressing A2aAR+BRIL GPCRDepositorInsertHomo sapiens adenosine A2a receptor (ADORA2A) (ADORA2A Human)
Tagscytochrome b562RILExpressionInsectPromotergp64Available SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC7
Plasmid#118876Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC151-3XUAS-IVS-Flp2::PEST
Plasmid#32133DepositorInsertFlippase2::PEST
TagsPESTExpressionInsectMutationGlycine at Amino Acid 5Available SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)
Plasmid#78509PurposeConstruct for expressing CD45R0 (extracellular domain) His10 in Sf9DepositorAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-20XUAS-IVS-PhiC31
Plasmid#133565PurposeReplace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.DepositorAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.His.XPB
Plasmid#39216DepositorAvailable SinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
Dynein-2_complex
Plasmid#132536PurposeFull length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.DepositorInsertsTags8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …ExpressionInsectMutationCodon optimised for Sf9 expressionAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
AE-Polyubiquitin-GCaMP6s
Plasmid#106868PurposeExpresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.DepositorInsertAE-Polyubiquitin-GCAaMP6s
ExpressionInsectPromoterAe. aegypti polyubiquitin (PUb)Available SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
PGRP-SA
Plasmid#21055DepositorAvailable SinceSept. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-LRRK2 WD40
Plasmid#139211PurposeHuman LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.DepositorAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(E17K)
Plasmid#86563PurposeExpresses human full-length Akt1(E17K) mutantDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKM50
Plasmid#123656PurposeEncodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cellsDepositorInsertAedes aegypti UbL40 promoter
UseLuciferaseExpressionInsectPromoterUbL40 promoterAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Human IntS6L
Plasmid#198409PurposeExpresses FLAG-tagged human IntS6LDepositorInsertIntegrator complex subunit 6 like (INTS6L Human)
Tags3x FLAGExpressionInsectPromoterUbi-p63eAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
Plasmid#217513PurposeEncodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD RecombinaseDepositorInsert10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
ExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4
Plasmid#217893PurposeInsect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)DepositorExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.His.XPD
Plasmid#39217DepositorInsertXPD (ERCC2 Human)
TagsHis (HHYHHH)ExpressionInsectMutationXPD starts at aa4PromoterPphAvailable SinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - CA
Plasmid#65424PurposeRhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63LDepositorInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, Q63L in RhoCAvailable SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - DN
Plasmid#65423PurposeRhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19NDepositorInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, T19N in RhoCAvailable SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBacI-KDAC6
Plasmid#224346PurposeExpresses human KDAC6 (HDAC6) in insect cellsDepositorAvailable SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTV-Gal4DBD2xHighMag1
Plasmid#182023PurposeDonor plasmid for conversion of Gal4 line into ShineGal4 driversDepositorInsertGal4DBD2xHighMag1
UseDonor plasmidExpressionInsectAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
AT5G63930 X059_pECIA2
Plasmid#115116PurposeBait vector AT5G63930 X059_pECIA2 should be used with prey vector AT5G63930 X059_pECIA14.DepositorInsertAT5G63930 (AT5G63930 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT1G17230 X074_pECIA2
Plasmid#115096PurposeBait vector AT1G17230 X074_pECIA2 should be used with prey vector AT1G17230 X074_pECIA14.DepositorInsertAT1G17230 (AT1G17230 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT2G33170 X049_pECIA2
Plasmid#115101PurposeBait vector AT2G33170 X049_pECIA2 should be used with prey vector AT2G33170 X049_pECIA14.DepositorInsertAT2G33170 (AT2G33170 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT1G74360 X038_pECIA2
Plasmid#115080PurposeBait vector AT1G74360 X038_pECIA2 should be used with prey vector AT1G74360 X038_pECIA14.DepositorInsertAT1G74360 (AT1G74360 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-FLPo-3xP3-RFP
Plasmid#127561PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.DepositorInsertFLPo
UseCRISPRExpressionInsectAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only