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Mammalian Expression

75,914 results

Showing: 5201 - 5240 of 75914 results

  1. IDG_SCN2B_OE_1

    Plasmid
    #161624
    Purpose
    Overexpresses SCN2B
    Depositor
    Mike McManus
    Insert
    SCN2B (SCN2B Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    161624-plasmid-map-sequence-id-334519

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  8. pcDNA3-NGFP-VMAn-FKBP

    Plasmid
    #70225
    Purpose
    Expresses N-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Use
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Glu 125 to Ile, Deleted amino acids 129-2…
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pcDNA3-FRB-VMAc-CGFP

    Plasmid
    #70226
    Purpose
    Expresses C-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Use
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Ile 129 to Cys, Deleted amino acids 1-128
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. IDG_SCN3B_OE_1

    Plasmid
    #161625
    Purpose
    Overexpresses SCN3B
    Depositor
    Mike McManus
    Insert
    SCN3B (SCN3B Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    161625-plasmid-map-sequence-id-319552

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  20. pMSCV-BLU-VIPR

    Plasmid
    #220497
    Purpose
    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Article
    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220497-plasmid-map-sequence-id-442817

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  21. pBLU-VIPR

    Plasmid
    #220498
    Purpose
    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Article
    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.
    Type
    Empty backbone
    Use
    CRISPR; Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220498-plasmid-map-sequence-id-442816

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  24. TFORF3532

    Plasmid
    #145008
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LIN28B (LIN28B Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145008-plasmid-map-sequence-id-287138

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  32. pPLN-hFluc-WT-HA-GFP11-KDEL

    Plasmid
    #177722
    Purpose
    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFluc
    Use
    Tags
    GFP11, HA, KDEL, and Prolactin Signal Sequence
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177722-plasmid-map-sequence-id-352603

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  34. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122239-plasmid-map-sequence-id-292012

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  37. mTurquoise-Rab5a-7

    Plasmid
    #55578
    Purpose
    Localization: Ras GTPase / Endosomes, Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    Rab5a
    Use
    Tags
    mTurquoise
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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Showing: 5201 - 5240 of 75914 results