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Mammalian Expression

76,373 results

Showing: 8601 - 8650 of 76373 results

  2. pMH004_phiC31_neo-HS4-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179432
    Purpose
    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    HS4-TRE-cit-HS4-mch (DH)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    179432-plasmid-map-sequence-id-362555

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  4. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40

    Plasmid
    #100833
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    GENIE Project, Douglas Kim
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    100833-plasmid-map-sequence-id-198271

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  5. G-CaMP3

    Plasmid
    #22692
    Purpose
    a single-wavelength GCaMP2-based genetically encoded calcium indicator
    Depositor
    Loren Looger
    Article
    Tian et al Nat Methods. 2009 Dec;6(12):875-81.
    Insert
    G-CaMP3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N363D mutation (increased fluorescence)
    Promoter
    Available since
    Nov. 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    22692-plasmid-map-sequence-id-241043

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  6. pLEX-MCS PGK promoter

    Plasmid
    #128044
    Purpose
    Lentiviral vector with PGK promoter
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128044-plasmid-map-sequence-id-311647

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  11. TFORF3038

    Plasmid
    #144514
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    IRF2 (IRF2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144514-plasmid-map-sequence-id-404224

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  12. pLenti CMVtight eGFP Puro (w771-1)

    Plasmid
    #26431
    Purpose
    2nd generation lentiviral vector
    Depositor
    Eric Campeau
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Lentiviral; Tet-on advanced vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    26431-plasmid-map-sequence-id-446955

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  24. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

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  32. MIGR-mFoxP3

    Plasmid
    #24067
    Depositor
    Dan Littman
    Insert
    Foxp3 (Foxp3 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24067-plasmid-map-sequence-id-473505

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  33. AAV.rTH.PI.Cre.SV40

    Plasmid
    #107788
    Purpose
    AAV vector expressing Cre from rTH promoter
    Depositor
    James M. Wilson
    Has Service
    AAV Retrograde and AAV9
    Insert
    Cre
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    rTH
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    107788-plasmid-map-sequence-id-259405

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  34. TFORF2058

    Plasmid
    #141898
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NKX2-2 (NKX2-2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141898-plasmid-map-sequence-id-390544

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  35. pcDNA3.1-GGamma5-GFP2

    Plasmid
    #166777
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma5-GFP2 (GNG5 Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166777-plasmid-map-sequence-id-408989

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  36. pcDNA3.1-GGamma3-GFP2

    Plasmid
    #166775
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma3-GFP2 (GNG3 Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166775-plasmid-map-sequence-id-330996

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  45. HTR1B-Tango

    Plasmid
    #66405
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HTR1B (HTR1B Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66405-plasmid-map-sequence-id-347879

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  47. pCS2-UTX-F-MT2

    Plasmid
    #40619
    Purpose
    Expression of enzyme-dead UTX in mammalian cells
    Depositor
    Kai Ge
    Article
    Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.
    Insert
    UTX (KDM6A Human)
    Use
    Tags
    6xMyc and Flag
    Expression
    Mammalian
    Mutation
    changed Histidine 1146 and Glutamic Acid 1148 to …
    Promoter
    CMV
    Available since
    Nov. 5, 2012
    Availability
    Academic Institutions and Nonprofits only

  48. pcDNA3-TRIF-CFP

    Plasmid
    #13644
    Depositor
    Doug Golenbock
    Insert
    TRIF (TICAM1 Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13644-plasmid-map-sequence-id-7722

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Showing: 8601 - 8650 of 76373 results