76,418 results

1. pGL2-AN

   Plasmid

   #20950

   Depositor

   William Kaelin

   Article

   Neuman et al Mol Cell Biol. 1994 Oct . 14(10):6607-15

   Insert

   E2F-1 promoter, genomic fragment (E2F1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   wt human E2F-1 promoter sequence

   Promoter

   Available since

   Aug. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTriEx-mCherry-PA-Rac1-T17N

   Plasmid

   #22029

   Purpose

   Expression of photoactivatable Rac1 (dominant-negative mutant).

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
   

   Use

   Tags

   6xHis and mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

   Promoter

   CMV, p10

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FLX1.8CMVMitoTurboRFP

   Plasmid

   #28131

   Depositor

   Geoffrey Owens

   Article

   Chen et al PLoS One. 2008 . 3(7):e2804.

   Insert

   MitoTurboRFP
   

   Use

   Lentiviral

   Tags

   COX VIII signal sequence

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.Syn.NES-jRCaMP1b.WPRE.SV40

   Plasmid

   #100851

   Purpose

   AAV expression of jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1 and AAV9

   Insert

   jRCaMP1b
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHR_TRE3GS-->sGFP-P2A-mCherry

   Plasmid

   #162232

   Purpose

   Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-mCherry in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   EGFP and mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF1245

   Plasmid

   #141805

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PKNOX2 (PKNOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SpCas9-NG

   Plasmid

   #138566

   Purpose

   Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSBBi-Pur LaG17-synNotch-Gal4VP64

   Plasmid

   #158135

   Purpose

   Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

   Depositor

   Neal Devaraj

   Insert

   LaG17-synNotch-Gal4VP64
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pARF1(T31N)-CFP

   Plasmid

   #11384

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   ADP Ribosylation Factor 1 (ARF1 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Changed Threonine 31 to Asparagine.

   Promoter

   Available since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221_SLC1A5

    Plasmid

    #131974

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC1A5 (SLC1A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. TFORF0630

    Plasmid

    #142668

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1I2 (NR1I2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Spectrin-C-cpstFRET

    Plasmid

    #61111

    Purpose

    Expression of Spectrin-C-term cpstFRET to measure mechanical stress and report multidimensional stress fields

    Depositor

    Fred Sachs

    Article

    Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

    Insert

    spectrin-cpstFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmCherry-ATG5

    Plasmid

    #13095

    Depositor

    Roberta Gottlieb

    Article

    Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

    Insert

    ATG5 (Atg5 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-cdk5GFP

    Plasmid

    #1346

    Depositor

    Li-Huei Tsai

    Insert

    CDK5 (CDK5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Flag-Vac 14 Full Length

    Plasmid

    #47418

    Depositor

    Peter McPherson

    Article

    Lemaire et al FEBS Lett. 2006 Dec 22;580(30):6948-54.

    Insert

    Vac14 (VAC14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

16. Tol2 8xSTAR-mScarletI-NLS. PGK-puro

    Plasmid

    #136263

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mScarletI-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. #1.4 pre-G-SynapShot-TagBFP

    Plasmid

    #214717

    Purpose

    Expresses green #1.4 pre-SynapShot and TagBFP

    Depositor

    Won Do Heo

    Article

    Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

    Insert

    #1.4 pre-G-SynapShot
    

    Use

    AAV

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Neurofascin-186-HA

    Plasmid

    #31061

    Depositor

    Vann Bennett

    Article

    Zhang et al J Biol Chem. 1998 Nov 13. 273(46):30785-

    Insert

    Neurofascin (Nfasc Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

19. EGFP-hPMCA4b

    Plasmid

    #47589

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA4x/b (ATP2B4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K442R and P673L relative to NP_001675.3

    Promoter

    CMV

    Available since

    Aug. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCh-KIF17*-strep

    Plasmid

    #120167

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 17 (KIF17 Human)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF17: 1-509 aa

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-BAZ2A

    Plasmid

    #65373

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BAZ2A (BAZ2A Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP-rGBD

    Plasmid

    #26732

    Depositor

    William Bement

    Article

    Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

    Insert

    rhotekin (rtkn2 Mouse)
    

    Use

    Reporter for rho

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    RhoA binding domain of rhotekin

    Promoter

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1_Floxed-STOP Fluc

    Plasmid

    #122962

    Purpose

    Firefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reaction

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_Floxed-STOP Fluc
    

    Use

    Cre/Lox and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YAP1 (S6A) - V5 in pLX304

    Plasmid

    #42562

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S61A, S109A, S127A, S128A, S131A, S163A, S164A, S…

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.3-hALDOA

    Plasmid

    #210809

    Purpose

    For transient transfection

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Human IgM-Fc

    Plasmid

    #158214

    Purpose

    Express the Fc region of human IgM

    Depositor

    Junyu Xiao

    Article

    Li et al Science. 2020 Feb 28;367(6481):1014-1017

    Insert

    IGHM (IGHM Human)
    

    Use

    Tags

    twin strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNAI-GAL4-CREB-S133A

    Plasmid

    #46770

    Purpose

    Expression of GAL4 fusion to CREB-S133A

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    GAL4(1-147)-CREB-S133A (Creb1, GAL4 Rat, Budding Yeast)
    

    Use

    Tags

    GAL4

    Expression

    Mammalian

    Mutation

    Changed CREB serine 133 to Alanine

    Promoter

    CMV

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCerulean3-TOMM20-N-10

    Plasmid

    #55449

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

29. HA-Clover-HIF-1alpha Wildtype

    Plasmid

    #163365

    Purpose

    Express HA- and Clover-tagged HIF-1alpha wildtype plasmid

    Depositor

    Violaine Sée

    Insert

    Hypoxia Inducible Factor 1 alpha (HIF1A Human)
    

    Use

    Tags

    HA- and Clover-tagged

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    CMV Promoter

    Available since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. H2B-Venus

    Plasmid

    #20971

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    Histone cluster 1, H2bb (H2BC3 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV-DeltaFosB

    Plasmid

    #68545

    Purpose

    AAV expression of DeltaFosB

    Depositor

    Eric Nestler

    Article

    Zachariou et al Nat Neurosci. 2006 Feb;9(2):205-11. Epub

    Insert

    DeltaFosB-IRES-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    truncated splice variant of FosB

    Promoter

    CMV

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

32. pLSSmKate1-N1

    Plasmid

    #31866

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    LSS-mKate1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Promoter

    CMV

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPGKFLPobpA

    Plasmid

    #13793

    Purpose

    Expression of mouse codon-optimized FLP recombinase with bovine growth hormone polyadenylation (bpA) sequence.

    Depositor

    Philippe Soriano

    Article

    Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

    Insert

    FLPo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

    Plasmid

    #89608

    Purpose

    Expression of Luciferase in human cells. 3rd generation lentiviral vector

    Depositor

    Fernando Fierro

    Article

    Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MNDU3

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLVX-opto-mCasp8

    Plasmid

    #208782

    Purpose

    Inducibly expresses mouse caspase8 fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    mCherry mCaspase8 Cry2 (Casp8 Mouse)
    

    Use

    Lentiviral

    Tags

    Cry2 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MSCV H-Ras V12 IRES GFP

    Plasmid

    #18780

    Depositor

    Scott Lowe

    Insert

    H-Ras V12 (HRAS Human)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    V12

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRK7-FLAG-TSC1

    Plasmid

    #8995

    Depositor

    John Blenis

    Article

    Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

    Insert

    TSC1 (TSC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAVS1-Nst-CAG-DEST

    Plasmid

    #80489

    Purpose

    Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPLN-hFluc-WT-HA-GFP11-KDEL

    Plasmid

    #177722

    Purpose

    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    GFP11, HA, KDEL, and Prolactin Signal Sequence

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-PE2-SpRY

    Plasmid

    #159979

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpRY
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pmScarlet_H2A_C1

    Plasmid

    #85051

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    H2A (H2AC16 Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1-FGFR2c-V5/HIS

    Plasmid

    #201107

    Purpose

    expression of human FGFR2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. TFORF2593

    Plasmid

    #142336

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BHLHE40 (BHLHE40 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. FR_HNF4A2

    Plasmid

    #31100

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

    Plasmid

    #194278

    Purpose

    Expresses gRNA and dSaCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

    Inserts

    humanized dSaCas9 KRAB T2A Thy1.1
    gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hUbC

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCI neo-hEST2

    Plasmid

    #1781

    Depositor

    Bob Weinberg

    Article

    Meyerson et al Cell 1997 Aug 22;90(4):785-95.

    Insert

    hTERT (TERT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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47. pB-Neo-mtkeima

    Plasmid

    #210338

    Purpose

    Overexpression of mtkeima

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    mtkeima
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNAI-GAL4-CREB-S142A

    Plasmid

    #46771

    Purpose

    Expression of GAL4 fusion to CREB-S142A

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    GAL4(1-147)-CREB-S142A (Creb1, GAL4 Rat, Budding Yeast)
    

    Use

    Tags

    GAL4

    Expression

    Mammalian

    Mutation

    Changed CREB serine 142 to Alanine

    Promoter

    CMV

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMCherry F8-2

    Plasmid

    #186034

    Purpose

    Express VHH F8-2 as a mCherry fusion in mammalian cells

    Depositor

    Isabelle Landrieu

    Article

    Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

    Insert

    VHH F8-2
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

    Plasmid

    #175508

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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