76,418 results

1. hSyn-DIO-somBiPOLES-mCerulean

   Plasmid

   #154951

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Has Service

   AAV Retrograde, AAV5, and AAV9

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GRK2-CAAX

   Plasmid

   #166224

   Purpose

   GRK2-CAAX mammalian expression plasmid

   Depositor

   Robert Lefkowitz

   Article

   Inglese et al Nature. 1992 Sep 10;359(6391):147-50. do

   Insert

   bovine GRK2-CAAX (GRK2 Bovine)
   

   Use

   Tags

   no

   Expression

   Mammalian

   Mutation

   adding CAAX sequence at C terminal

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-Neo-Bam p53 R249S

   Plasmid

   #16438

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 R249S (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R249S

   Promoter

   Available since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-Tag4-OMA1

   Plasmid

   #61719

   Purpose

   mammalian expression of human Oma1 with C-terminal FLAG tag

   Depositor

   Oleh Khalimonchuk

   Insert

   OMA1 (OMA1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-centrin-1

   Plasmid

   #72641

   Purpose

   The plasmid encodes centrin-1 with an N-terminal EGFP tag

   Depositor

   Michel Bornens

   Insert

   centrin-1 (CETN1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. POLD1_pLX307

   Plasmid

   #98358

   Purpose

   Lentiviral expression of POLD1

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   POLD1 (POLD1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCh-KIF13A*-strep

   Plasmid

   #120166

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 13A (KIF13A Human)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIF13A: 1-639 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mTurquoise-Rab5a-7

   Plasmid

   #55578

   Purpose

   Localization: Ras GTPase / Endosomes, Excitation: 434, Emission: 474

   Depositor

   Michael Davidson

   Insert

   Rab5a
   

   Use

   Tags

   mTurquoise

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. hNFAT2-409:TALE:VP64:KRɸ

   Plasmid

   #160661

   Purpose

   Constitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

   Depositor

   Roman Jerala

   Article

   Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

   Insert

   hNFAT2-409:TALE:VP64:KRɸ
   

   Use

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

    Plasmid

    #87694

    Purpose

    Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    ERT2-PhoCl-Cre-PhoCl-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCherry-Alpha-5-Integrin-12

    Plasmid

    #54970

    Purpose

    Localization: Focal Adhesions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Alpha-5 Integrin (ITGA5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMJ179

    Plasmid

    #85996

    Purpose

    One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    EGFP-NT2_cr2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified mouse U6

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSinREP5-iAchSnFR

    Plasmid

    #140643

    Purpose

    Acetylcholine sensor for sindbis virus

    Depositor

    J. Julius Zhu

    Article

    Borden et al bioRxiv 2020

    Insert

    Ach sensor V9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AgDD

    Plasmid

    #78289

    Purpose

    create protein aggregates in living cells

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MLALKLAGLDI

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

    Plasmid

    #55613

    Purpose

    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (WT) (HERC2 Human)
    

    Use

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    Silent mutations have been incorporated into the …

    Promoter

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV-U6+27-Squash

    Plasmid

    #177913

    Purpose

    Expresses Squash aptamer in mammalian cells using the U6+27 promoter.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Squash aptamer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiBE3-SpRY-Blast

    Plasmid

    #199303

    Purpose

    Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    FNLS-BE3-SpRY
    

    Use

    CRISPR and Lentiviral

    Tags

    2A tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-SBP-VAP-A*

    Plasmid

    #120175

    Purpose

    Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    VAMP associated protein A (VAPA Human)
    

    Use

    Tags

    EGFP and SBP

    Expression

    Mammalian

    Mutation

    Truncated VAP-A: 118-249

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. qTAG-C-mStayGold-Puro-PXN

    Plasmid

    #227317

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    PXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB-cT3G-cERP2-MAFF

    Plasmid

    #192917

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of MAFF

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    MAFF (MAFF Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pShuttle-mRFP-gsk3 s9a

    Plasmid

    #24371

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    GSK3beta(S9A) (GSK3B Human)
    

    Use

    Adenoviral

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    S9A. Mutant version of GSK3 that lacks the inhibi…

    Promoter

    Available since

    May 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCF142_U6-sgRNA

    Plasmid

    #225960

    Purpose

    U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    U6-sgRNA (recipient)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pU6-sp-sgRNA-RNF2_+41nick

    Plasmid

    #135958

    Purpose

    S. pyogenes sgRNA for +41 nick in RNF2

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    RNF2_+41 nicking sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-ChrimsonR-tdT

    Plasmid

    #59169

    Purpose

    Expression vector for ChrimsonR-tdTomato

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    CAG

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. lenti-fullef1a-FAKSPARK2

    Plasmid

    #195265

    Purpose

    express wtFAKSPARK2 in a lenti vector

    Depositor

    Xiaokun Shu

    Article

    Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

    Insert

    FAKSPARK2 sensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ef1a

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mCh-BICD2*-strep

    Plasmid

    #120168

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    BICD cargo adaptor 2 (Bicd2 Mouse)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated BICD2: 15-595 aa

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

    Plasmid

    #35510

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 Mfn2-HA S27A

    Plasmid

    #139193

    Purpose

    Expresses C-terminal HA tagged human Mfn2 S27A in mammalian cells

    Depositor

    Allan Weissman

    Article

    Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

    Insert

    MFN2 (MFN2 Human)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    Serine 27 to Alanine

    Promoter

    CMV promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAV-5S-Squash

    Plasmid

    #177914

    Purpose

    Expresses 5S rRNA fused to Squash aptamer in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Squash aptamer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5S

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1(+)-iBB-GECO1

    Plasmid

    #194215

    Purpose

    Far-red fluorescent Ca2+ ion indicator for mammalian cell expression.

    Depositor

    Jin Zhang

    Article

    Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

    Insert

    iBB-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFH2.217_PB-EPN24-PCP-IRES-eGFP-PGK-Blast

    Plasmid

    #205557

    Purpose

    Express EPN24-PCP-IRES-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-PCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HiBiT-SRPK1 Fusion Vector

    Plasmid

    #236908

    Purpose

    Express HiBiT-SRPK1 in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    SRPK1 (SRPK1 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pCDH-CMV-HA-CFP-SLMAP (isoform r)

    Plasmid

    #235684

    Purpose

    Express CFP tagged SLMAP gene in mammalian cells using the CMV promoter

    Depositor

    Duojia Pan

    Article

    Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

    Insert

    SLMAP (SLMAP Human)
    

    Use

    Lentiviral

    Tags

    HA-CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

    Plasmid

    #223965

    Purpose

    Dual fluorescent reporter for histone H2B and for Cdt1

    Depositor

    Hee Won Yang

    Article

    Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

    Insert

    H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670 and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CL20_mEGFP_BCL9L_CBL

    Plasmid

    #205779

    Purpose

    Express mEGFP-tagged fusion protein, BCL9L_CBL from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    BCL9L_CBL (BCL9L, CBL Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TFORF0938

    Plasmid

    #143083

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SS18 (SS18 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TFORF1166

    Plasmid

    #143471

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EBF4 (EBF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-nCFP

    Plasmid

    #185360

    Purpose

    For mammalian expression of N-term CFP for split-CFP

    Depositor

    Ayaz Najafov

    Insert

    CFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PIK3C2A gRNA (BRDN0001149181)

    Plasmid

    #76414

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3C2A

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3C2A (PIK3C2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PIK3C2A gRNA (BRDN0001148547)

    Plasmid

    #76415

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3C2A

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3C2A (PIK3C2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Vinculin-venus

    Plasmid

    #27300

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Vinculin (VCL Chicken)
    

    Use

    Tags

    Venus A206K

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.4_17G6-1 light chain

    Plasmid

    #198250

    Purpose

    recombinant expression of light chain of monoclonal anti-AMP antibody 17G6-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    light chain of monoclonal anti-AMP antibody 17G6-1 (mouse kappa)
    

    Use

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

    Plasmid

    #193968

    Purpose

    Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    hPol eta (POLH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. TFORF3514

    Plasmid

    #144990

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PURB (PURB Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCS2-UTX-F

    Plasmid

    #17438

    Purpose

    Expression of UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    UTX (KDM6A Human)
    

    Use

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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46. pMDG

    Plasmid

    #187440

    Purpose

    VSV-G envelope expressing plasmid

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Inserts

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. BII-Sh-FUCCI

    Plasmid

    #133363

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Clover-Geminin-IRES-mKO2-Cdt1
    

    Use

    Tags

    HA-tagged Clover-Geminin

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. dmBACCS2-IRES-dOrai-IRES-mCherry

    Plasmid

    #72894

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCI-SEP_NR2A

    Plasmid

    #23997

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    NR2A (Grin2a Rat)
    

    Use

    Tags

    SEP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCX-DI-2A

    Plasmid

    #74671

    Purpose

    Mammalian expression of DI-2A (hCD73/F2A/hCD39)

    Depositor

    Roberto Giovannoni

    Article

    De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

    Inserts

    CD73 (NT5E Human)
    CD39 (ENTPD1 Human)
    

    Use

    Tags

    F2A

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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