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82,461 results

Showing: 41 - 60 of 82461 results

  1. pHR-SFFV-dCas9-BFP-KRAB

    Plasmid
    #46911
    Purpose
    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain
    Depositor
    Stanley Qi, Jonathan Weissman
    Publication
    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008
    Insert
    dCas9-BFP-KRAB fusion
    Use
    CRISPR and Lentiviral
    Tags
    2xNLS, BFP, HA, and KRAB domain
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    121 Articles
    46911-plasmid-map-sequence-id-449615

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  4. pLVTHM

    Plasmid
    #12247
    Purpose
    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid
    Depositor
    Didier Trono
    Publication
    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194 Articles
    12247-plasmid-map-sequence-id-352425

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  5. ptfLC3

    Plasmid
    #21074
    Purpose
    Mammalian expression of rat LC3 fused to mRFP and EGFP
    Depositor
    Tamotsu Yoshimori
    Publication
    Kimura et al Autophagy. 2007;3(5):452-60.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    Tags
    EGFP and mRFP
    Expression
    Mammalian
    Available since
    May 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    331 Articles
    21074-plasmid-map-sequence-id-324322

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  6. pSpCas9n(BB)-2A-Puro (PX462)

    Plasmid
    #48141
    Purpose
    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9n-2A-Puro
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Cas9 D10A nickase mutant
    Promoter
    Cbh
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75 Articles

  7. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid
    #36047
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag
    Depositor
    Kyle Roux
    Publication
    Roux et al J Cell Biol. 2012 Mar 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BirA(R118G)-HA
    Tags
    HA
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Available since
    April 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65 Articles
    36047-plasmid-map-sequence-id-381279

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  10. MCS-BioID2-HA

    Plasmid
    #74224
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68 Articles
    74224-plasmid-map-sequence-id-238509

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  11. eSpCas9(1.1)

    Plasmid
    #71814
    Purpose
    Expresses high specificity SpCas9. Px330-like plasmid.
    Depositor
    Feng Zhang
    Publication
    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    141 Articles
    71814-plasmid-map-sequence-id-177023

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  17. pcDNA3.1 mycBioID

    Plasmid
    #35700
    Purpose
    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag
    Depositor
    Kyle Roux
    Publication
    Roux et al J Cell Biol. 2012 Mar 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BirA
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    cmv
    Available since
    April 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    94 Articles
    35700-plasmid-map-sequence-id-228311

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  20. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    119 Articles
    61427-plasmid-map-sequence-id-322387

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Showing: 41 - 60 of 82461 results