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pLVX-TetOne-Puro-GFP

Plasmid

#171123

Purpose

Doxycycline inducible expression of GFP

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3GS

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

Plasmid

#171588

Purpose

Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

Bxb1 integrase
iCasp9
BFP
rtTA3G

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi-GP

Plasmid

#60511

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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SpRY-Tad-CBE(ACC3)

Plasmid

#235082

Purpose

Expresses SpRY-Tad-CBE(ACC3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACC3)-SpRY Cas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL-CD86 mIL-15

Plasmid

#183245

Purpose

Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV FKBP-V5-sTurboID (N)

Plasmid

#153002

Purpose

Expresses low-affinity split-TurboID N-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID (N) (L73)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV HA-HaloTag-FRB-sTurboID (C)

Plasmid

#153003

Purpose

Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID (C) (G74)

Use

Lentiviral

Tags

FRB, HA, and HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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H2B-mCherry

Plasmid

#20972

Purpose

H2B fused to mCherry for chromatin visualization

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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JD171

Plasmid

#229483

Purpose

Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E2A, VA RNA I, L4-22k/33k

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

CMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-mitoDsRed

Plasmid

#44386

Purpose

3rd generation lentiviral vector

Depositor

Pantelis Tsoulfas

Article

Kitay et al Hum Mol Genet. 2013 Jan 24.

Insert

mitoDsRed

Use

Lentiviral

Tags

61 aa targeting sequence of the P1 isoform F1F0-A…

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1

Plasmid

#54767

Purpose

Localization: N1 Cloning Vector, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

mEGFP

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSBbi-RP

Plasmid

#60513

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGlucoSnFR-TS

Plasmid

#127952

Purpose

Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

Depositor

Gary Yellen

Article

Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

Insert

iGlucoSnFR-TS

Use

AAV

Tags

His6 and Xpress epitope

Expression

Mammalian

Promoter

CAG

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pKrox24(MapErk)DsRed

Plasmid

#200114

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

MapErk sequence in minimal promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

5 copies of designed MapErk sequence, no other pr…

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR2

Plasmid

#64932

Purpose

Enhanced FRET-based biosensor for monitoring Akt activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

AktAR2

Tags

Cerulean3 and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL-CD86

Plasmid

#183244

Purpose

Expression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

CD86, 41BBL

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA AXL

Plasmid

#105932

Purpose

Mammalian expression of myc-His tagged AXL

Depositor

Rosa Marina Melillo

Article

Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

Insert

AXL (AXL Human)

Tags

His and myc

Expression

Mammalian

Promoter

tet-inducible CMV

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry

Plasmid

#128744

Purpose

Expresses mCherry in mammalian cells

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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scramble shRNA

Plasmid

#1864

Purpose

3rd gen lentiviral negative control vector containing scrambled shRNA

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

scramble

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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Mitochondrial PancACe

Plasmid

#215710

Purpose

Expresses acetyl-CoA biosensor in the mitochondria

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and mitochondrial localization sequence…

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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