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Plasmid#222339PurposePlasmid for expressing evolved and engineered Bxb1 in the mammalian cellsDepositorInsertCodon-optimized evolved and engineered Bxb1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dR8.2 dvpr
Plasmid#8455Purpose2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT-IRES-Puro
Plasmid#182590Purposeexpresses TERT in mammalian cellsDepositorInserthTERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CA
Plasmid#20960PurposepiggyBac empty vector with Gatweway cassette and CAG constitutive promoterDepositorInsertGateway Destination Vector
UsepiggybacTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNGN2
Plasmid#172115PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expressionInsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti dCAS-VP64_Blast
Plasmid#61425Purpose3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)DepositorHas ServiceLentiviral PrepInsertdCAS9(D10A, N863A)-VP64_2A_Blast
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1AAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
scAAV-sgRNA-GFP
Plasmid#177935PurposeExpresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic miceDepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
FH-TET1-pEF
Plasmid#49792PurposeThis plasmid expresses the full-length human TET1 gene with a Flag and HA tag.DepositorInsertTet methylcytosine dioxygenase 1 (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hOCT3/4-shp53-F
Plasmid#27077PurposeIntegration-free (episomal) expression of human OCT3/4 and shRNA against p53DepositorInsertOCT3/4 (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
FUGW
Plasmid#14883Purpose3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresionDepositorInsertflap-Ub promoter-GFP-WRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac PlayBack
Plasmid#203312PurposeTo all for PiggyBac gene insertion into GenomeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
HDAC1 Flag
Plasmid#13820PurposeMammalian expression of human histone deacetylase 1 with flag tagDepositorInsertHDAC1 (HDAC1 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GFP
Plasmid#11153PurposeMammalian expression vector for expression of GFP (CMV promoter)DepositorHas ServiceCloning Grade DNAInsertCMV promoter-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVX-UbC-rtTA-Ngn2:2A:Ascl1
Plasmid#127289Purposeall-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)DepositorUseLentiviralTagsNgn2 and Ascl1 linked via 2AExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL2-p21 promoter-Luc
Plasmid#33021DepositorInsertp21 promoter (CDKN1A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterp21Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLX_305_Ovalbumin
Plasmid#184924PurposeLentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.DepositorInsertOvalbumin (OVAL Chicken)
UseLentiviralTagsExpressionMammalianMutationOur vector has an A188T mutation that does not im…PromoterPGKAvailable sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
XLone-GFP
Plasmid#96930PurposeTunable and temporal expression control of GFPDepositorInsertGreen Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hUL
Plasmid#27080PurposeIntegration-free (episomal) expression of human L-MYC and LIN28DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIG-FLAG-MLL-AF9
Plasmid#71443PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFPDepositorInsertMLL-Af9 (KMT2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only