78,292 results

1. pCMV-PE6a

   Plasmid

   #207851

   Purpose

   Mammalian expression of PE6a prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6a
   

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE-Tight-MitoTimer

   Plasmid

   #50547

   Purpose

   Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

   Depositor

   Roberta Gottlieb

   Article

   Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

   Insert

   MitoTimer
   

   Expression

   Mammalian

   Mutation

   F12S mutation in Timer

   Available Since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

   Plasmid

   #102796

   Purpose

   Retrovirus for delivery of one sgRNA (empty, bbsI)

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   U6 sgRNA
   

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide Puro-P2A-EGFP

   Plasmid

   #137729

   Purpose

   For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-AR

   Plasmid

   #28235

   Purpose

   Mammalian expression of Androgen receptor fused to EGFP

   Depositor

   Michael Mancini

   Article

   Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

   Insert

   Androgen receptor (AR Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-SRRM2-mCherry

   Plasmid

   #174089

   Purpose

   Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

   Depositor

   Wesley Sundquist, Katie Ullman

   Article

   Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

   Insert

   SRRM2 (SRRM2 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TREtight

   Available Since

   Sept. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. ER-GCaMP6-150

   Plasmid

   #86918

   Purpose

   Fluorescent reporter for ER calcium signaling

   Depositor

   Timothy Ryan

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   ER-GCaMP6-150
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

   Promoter

   CAMKII

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

   Plasmid

   #113610

   Purpose

   PPI reporter

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

   Insert

   beta 2 adrenergic receptor
   

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-ATG5

   Plasmid

   #13095

   Depositor

   Roberta Gottlieb

   Article

   Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

   Insert

   ATG5 (Atg5 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Nov. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. hCas9

    Plasmid

    #41815

    Purpose

    Expresses human codon optimized Cas9 nuclease for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-Tol2

    Plasmid

    #31823

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Insert

    Tol2 Transposase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. OMMGFP 1-10

    Plasmid

    #164119

    Purpose

    Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

    Depositor

    Marisa Brini, Tito Calì

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    OMMGFP 1-10
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF1922

    Plasmid

    #141888

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZBTB20 (ZBTB20 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

    Plasmid

    #205703

    Purpose

    CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-EGFP-RhoA-Q63L

    Plasmid

    #12968

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA constitutively active (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q63L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1+_hsTRPA1_myc_dTom

    Plasmid

    #183179

    Purpose

    myc tagged hsTRPA1 used for cell transfection

    Depositor

    Sreekanth Chalasani

    Article

    Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

    Inserts

    hsTRPA1 (TRPA1 Human)
    dTom
    

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF2971

    Plasmid

    #144447

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1H4 (NR1H4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF3039

    Plasmid

    #144515

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1H3 (NR1H3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-Hylight

    Plasmid

    #193447

    Purpose

    A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

    Depositor

    Richard Goodman

    Article

    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Hylight Biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

    Plasmid

    #208696

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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