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Mammalian Expression

76,416 results

Showing: 2441 - 2480 of 76416 results

  6. pLEX_305

    Plasmid
    #41390
    Purpose
    Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41390-plasmid-map-sequence-id-352470

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  7. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

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  17. TFORF0915

    Plasmid
    #143451
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NCOA2 (NCOA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143451-plasmid-map-sequence-id-397908

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  18. pCW57.1_blasti

    Plasmid
    #194067
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194067-plasmid-map-sequence-id-380706

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  20. pHS2002

    Plasmid
    #239832
    Purpose
    Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    NovaIscB omegaRNA scaffold
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Replacement of A12-A57 with GAAA in omegaRNA scaf…
    Promoter
    human U6
    Available since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239832-plasmid-map-sequence-id-479110

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  26. pcDNA3.1-Flag

    Plasmid
    #208051
    Purpose
    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208051-plasmid-map-sequence-id-418310

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  28. pCAGGs-BFP

    Plasmid
    #127348
    Purpose
    Expresses BFP constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Phil Sharp
    Insert
    Blue Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS Promoter
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    127348-plasmid-map-sequence-id-246576

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  29. MCS-13X Linker-BioID2-HA

    Plasmid
    #80899
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    80899-plasmid-map-sequence-id-181185

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  34. TFORF2946

    Plasmid
    #144422
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYOD1 (MYOD1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144422-plasmid-map-sequence-id-407317

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Showing: 2441 - 2480 of 76416 results