76,055 results

1. pEGFP-N1-1x

   Plasmid

   #172281

   Purpose

   Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

   Depositor

   Antony K Chen

   Article

   Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

   Insert

   EGFP-N1-1x
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK5-HA-Parkin

   Plasmid

   #17613

   Depositor

   Ted Dawson

   Article

   Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBABE-MCS(extended)-Puro (empty vector)

   Plasmid

   #172603

   Purpose

   Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The multiple cloning site (MCS) has been edited/e…

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-C2_SEPT9 i4

   Plasmid

   #71617

   Purpose

   mammalian expression of human SEPT9 i4 with a GFP fusion

   Depositor

   Cristina Montagna, Elias Spiliotis

   Article

   Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

   Insert

   Septin 9 (SEPTIN9 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-EGFP-RhoA-Q63L

   Plasmid

   #12968

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   RhoA constitutively active (RHOA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q63L Constitutively active

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HRE-dUnOHR

   Plasmid

   #201714

   Purpose

   Hypoxia responsive promoter driving UnaG and mOrange

   Depositor

   Friedemann Kiefer

   Article

   Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-Lyn-AKAR4

   Plasmid

   #61620

   Purpose

   Lipid raft-targeted FRET biosensor for monitoring PKA activity

   Depositor

   Jin Zhang

   Article

   Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

   Insert

   Lyn-AKAR4
   

   Use

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAGGS-ACE2-his expression plasmid

   Plasmid

   #158089

   Purpose

   Expresses ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-Gα13*-BERKY3

   Plasmid

   #158218

   Purpose

   BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pDEST_FLAG-HUWE1

    Plasmid

    #187120

    Purpose

    mammalian cell expression of FLAG-HUWE1

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLX209-neo-active WRN

    Plasmid

    #125788

    Purpose

    constitutive, ectopic expression of human active WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-active WRN (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pINDUCER20-Tau

    Plasmid

    #92201

    Purpose

    Retroviral overexpression vector (doxycycline-inducible) for Tau

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221_SLC22A5

    Plasmid

    #132048

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC22A5 (SLC22A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCMV5B-HA-Smad2

    Plasmid

    #11734

    Depositor

    Jeff Wrana

    Article

    Eppert et al Cell. 1996 Aug 23. 86(4):543-52.

    Insert

    SMAD2 (SMAD2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. mKeima-Red-N1

    Plasmid

    #54597

    Purpose

    Localization: N1 Cloning Vector, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mKeima-Red

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmGFP-ADAR1-p110

    Plasmid

    #117928

    Purpose

    ADAR1-p110 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p110 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid

    #182287

    Purpose

    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    

    Use

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CaMKII-PinkyCaMP

    Plasmid

    #232858

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 5HRE/GFP

    Plasmid

    #46926

    Purpose

    hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

    Depositor

    Thomas Foster, Martin Brown

    Article

    Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

    Insert

    5X HRE of VEGF (VEGFA Human)
    

    Use

    Tags

    d2EGFP

    Expression

    Mammalian

    Mutation

    five copies of a 35-bp fragment from the hypoxia-…

    Promoter

    EF-1α promoter

    Available since

    Aug. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF3518

    Plasmid

    #144994

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NHLH2 (NHLH2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV10/3xflag-Clock

    Plasmid

    #47334

    Purpose

    full length clock in pCMV10 with 3x flag at the N-terminus

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    clock
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TFORF0481

    Plasmid

    #142638

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HIF1A (HIF1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p50 cFlag pcDNA3

    Plasmid

    #20018

    Depositor

    Stephen Smale

    Insert

    Nfkb1 (Nfkb1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Truncated at the p105 ligation site. It codes for…

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EF1a-TET1-dCas9

    Plasmid

    #235593

    Purpose

    Tet1CD fused to N-terminus of dCas9

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    TET1-dCas9 (TET1 Human, SpCas9 is from Streptococcus pyogenes)
    

    Use

    CRISPR

    Tags

    3xNLS, TET1 CD, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-TRE3g-mNeon-WPRE-pA

    Plasmid

    #192064

    Purpose

    mNeonGreen expression with TRE3g promoter

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3g

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHTC-HaloTag_C9orf72:1-481

    Plasmid

    #210987

    Purpose

    Mammalian expression of full-length human C9orf72. C-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    C9orf72:M1-F481 (C9orf72 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. GFP-Dcp1a

    Plasmid

    #153972

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Use

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mApple-LAMP1-phLuorin-N-8

    Plasmid

    #54918

    Purpose

    Localization: Lysosome Membrane, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    LAMP1 phLuorin
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Lysosomes-20 double labeled with phLuorin

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKH3-human RSK1

    Plasmid

    #13841

    Depositor

    John Blenis

    Article

    Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

    Insert

    RSK1 (RPS6KA1 Human)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRK5F-TACE

    Plasmid

    #31713

    Purpose

    Mammalian expression of human TACE (ADAM17)

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    TACE (ADAM17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. Rtn3L-mNeonGreen

    Plasmid

    #169034

    Purpose

    Express human Rtn3L in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rtn3L (RTN3 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR221_SLC9A2

    Plasmid

    #132222

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC9A2 (SLC9A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. Fuw-dCas9-Tet1CD_IM

    Plasmid

    #84479

    Purpose

    Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Tet1CD_IM
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV.TBG.PI.Null.bGH

    Plasmid

    #105536

    Purpose

    empty AAV vector with TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    None
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TBG

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EGFP-Rubicon

    Plasmid

    #28022

    Depositor

    Qing Zhong

    Article

    Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

    Insert

    Rubicon (RUBCN Human)
    

    Use

    Tags

    3xFlag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBV-Luc

    Plasmid

    #16539

    Purpose

    luciferase reporter plasmid with very low basal activity

    Depositor

    Bert Vogelstein

    Article

    He et al Cell. 1999 Oct 29. 99(3):335-45.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

    Plasmid

    #125235

    Purpose

    Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAG-hHDAC6

    Plasmid

    #21099

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    Histone Deacetylase 6 (HDAC6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

39. pMK265 (RAD21-mAC Hygro)

    Plasmid

    #140539

    Purpose

    RAD21 tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1

    Plasmid

    #122147

    Purpose

    Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Lentiviral

    Tags

    NLS-iLID-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCDNA3.1(+)-NEMOc

    Plasmid

    #189933

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3-ER-AKAR4

    Plasmid

    #64733

    Purpose

    Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    AKAR4
    

    Use

    Tags

    Cerulean, N-terminal 27 amino acids from CYP450, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLX401-INK4A

    Plasmid

    #121919

    Purpose

    Doxycycline-inducible expression of p16-INK4A product of CDKN2A

    Depositor

    William Hahn

    Article

    Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

    Insert

    p16-INK4A (CDKN2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Tet ON

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pmRFP-LC3

    Plasmid

    #21075

    Purpose

    Mammalian expression of rat LC3 fused to mRFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    First ATG is not included.

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. PCMV-flag-Caspase11

    Plasmid

    #21145

    Depositor

    Junying Yuan

    Article

    Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

    Insert

    Caspase11 (Casp4 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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46. UBC plasmid

    Plasmid

    #169746

    Purpose

    Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. Epac-S-H187

    Plasmid

    #170348

    Purpose

    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

    Insert

    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV-CASI-Cas9C

    Plasmid

    #80932

    Purpose

    Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. PB-CA

    Plasmid

    #20960

    Purpose

    piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLKO-MCS

    Plasmid

    #185594

    Purpose

    Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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