Skip to main content
Addgene
Showing: 601 - 650 of 7933 results

  14. pGJJ001

    Plasmid
    #183619
    Purpose
    bindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.
    Depositor
    Ben Lehner
    Article
    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183619-plasmid-map-sequence-id-361476

    Enlarge

  22. pCE41

    Plasmid
    #174433
    Purpose
    C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.
    Depositor
    Clarissa Nobile
    Article
    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182
    Insert
    HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174433-plasmid-map-sequence-id-349717

    Enlarge

  25. pRS303-PADH1-AFB2

    Plasmid
    #99530
    Purpose
    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)
    Depositor
    Helle Ulrich
    Insert
    AFB2 (AFB2 Mustard Weed)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99530-plasmid-map-sequence-id-190861

    Enlarge

  39. MmSphk1

    Plasmid
    #118599
    Purpose
    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Mus musculus sphingosine kinase 1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    118599-plasmid-map-sequence-id-234126

    Enlarge

  40. HsSPHK1

    Plasmid
    #118091
    Purpose
    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens Sphingosine Kinase 1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    118091-plasmid-map-sequence-id-234124

    Enlarge

  45. pE6n

    Plasmid
    #17460
    Purpose
    modified pENTR vector for N-terminal EYFP tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    EYFP
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17460-plasmid-map-sequence-id-8628

    Enlarge

  46. ZJOM143

    Plasmid
    #133667
    Purpose
    Integration of VPH1-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPH1-mTagBFP2 (VPH1 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133667-plasmid-map-sequence-id-265896

    Enlarge

  47. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

    Enlarge

  49. ZJOM40

    Plasmid
    #133650
    Purpose
    Integration of SEC7-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-SEC7-DuDre (SEC7 Budding Yeast)
    Use
    Tags
    DuDre
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    133650-plasmid-map-sequence-id-292148

    Enlarge

Showing: 601 - 650 of 7933 results