8,631 results

1. pDEST-hisMBP-AsCpf1-EC

   Plasmid

   #79007

   Purpose

   Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

   Depositor

   Jin-Soo Kim

   Article

   Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Insert

   AsCpf1
   

   Use

   Tags

   6xHis-MBP-NLS and NLS-HA

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Fuw-dCas9-Tet1CD_IM

   Plasmid

   #84479

   Purpose

   Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Tet1CD_IM
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lac-I-SceI-Tet

   Plasmid

   #17655

   Depositor

   Tom Misteli

   Article

   Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

   Insert

   lac I-SceI tet
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 30, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

   Plasmid

   #103866

   Purpose

   dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GSGGGGS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK-Myc-Smurf1

   Plasmid

   #13676

   Depositor

   Ying Zhang

   Article

   Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

   Insert

   Smurf1 (SMURF1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28HIS-hAPE1

   Plasmid

   #70757

   Purpose

   bacterial expression of human APE1

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   APEX1 (APEX1 Human)
   

   Use

   Tags

   6-HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lac operon

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. tet_pLKO.1_puro_shNRF2 #1

   Plasmid

   #136584

   Purpose

   Expresses an inducible short hairpin targeting human NRF2 sequence

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   shNRF2v1 (NFE2L2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRubiG-T2A-Cas9

   Plasmid

   #75348

   Purpose

   Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   GFP (via t2a)

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p1242 3x-KB-L

   Plasmid

   #26699

   Depositor

   Bill Sugden

   Article

   Mitchell et al J Virol. 1995 May . 69(5):2968-76.

   Insert

   3x MHC I KB element - luciferase (NFKB1 Human, Photinus pyralis)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK5-HA-Ubiquitin-K29R

    Plasmid

    #17602

    Purpose

    Mammalian expression of HA tagged ubiquitin K29R

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K29R; V5 deleted

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-EF1a-FLEX-GT

    Plasmid

    #26198

    Depositor

    Edward Callaway

    Article

    Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Enhanced green fluorescent protein, TVA (eGFP Mouse, TVA is a mouse codon optimized version of an avian gene.)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    TVA mammalian codon optimized

    Promoter

    Available since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. IKK-2 K44M

    Plasmid

    #11104

    Depositor

    Anjana Rao

    Article

    Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

    Insert

    IKK-2 (IKBKB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K44M, kinase inactive

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBiFC-CC155

    Plasmid

    #22015

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    CC155
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p18sheLL

    Plasmid

    #37321

    Depositor

    John Schiller

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    HPV18L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXs-hKLF4

    Plasmid

    #17219

    Purpose

    Retroviral expression of human KLF4

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-4T-3-mR7BD

    Plasmid

    #79149

    Purpose

    bacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)

    Depositor

    Aimee Edinger

    Article

    Romero Rosales et al Mol Biol Cell. 2009 Jun;20(12):2831-40.

    Insert

    R7BD (Rilp Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 220-299

    Promoter

    tac

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C5V

    Plasmid

    #26394

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Insert

    Cerulean-5-Venus
    

    Use

    Fret standard

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-SHP2 WT

    Plasmid

    #8381

    Depositor

    Ben Neel

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-Golgi eGFP

    Plasmid

    #79809

    Purpose

    To express and target GFP into the Golgi apparatus using lentiviruses

    Depositor

    Pantelis Tsoulfas

    Insert

    Golgi eGFP (B4GALT1 Human)
    

    Use

    Lentiviral

    Tags

    Fusion of the first 62 amino acids of the human b…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mcherry-PH

    Plasmid

    #36075

    Depositor

    Narasimhan Gautam

    Article

    Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

    Insert

    PH domain of PLCdelta1 (PLCD1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p3XFlag-CAPN1

    Plasmid

    #60941

    Purpose

    Expresses calpain1 in mammalian cells

    Depositor

    Yi Zhang

    Article

    Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

    Insert

    Calpain1 (CAPN1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNLS-iRFP720

    Plasmid

    #45467

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP720
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pShuttle-CMV-iRFP

    Plasmid

    #31856

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Insert

    iRFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid

    #86233

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEYFP-Mitotrap

    Plasmid

    #46942

    Purpose

    Expresses Mitotrap in mammalian cells

    Depositor

    Margaret Robinson

    Article

    Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

    Insert

    Mitotrap (MTOR Human, Aequorea victoria, Synthetic, Budding Yeast)
    

    Use

    Tags

    This is a fusion between a yeast mitochondrial ta…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PEA1-Puro

    Plasmid

    #171991

    Purpose

    Delivers all prime editing nickase components in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro

    Plasmid

    #141370

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pcDNA3.1_sfCherry2(1-10)

    Plasmid

    #82602

    Purpose

    Expresses sfCherry2(1-10) fusion in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Glu 118 to Gln, changed Thr 128 to Ile

    Promoter

    CMV

    Available since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA3.3_CoV2_B.1.1.7

    Plasmid

    #170451

    Purpose

    Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Yuan et al Science. 2021 Aug 13;373(6556):818-823.

    Insert

    SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …

    Promoter

    CMV

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMRXIP GFP-Stx17 WT

    Plasmid

    #45909

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    Syntaxin 17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-MBP-2xNLS-tdTomato

    Plasmid

    #104054

    Purpose

    An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    2xNLS-tdTomato
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    MBP

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. dClover2-N1

    Plasmid

    #54538

    Purpose

    Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

    Depositor

    Michael Davidson

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    dClover2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pDONR207 SARS-CoV-2 NSP3

    Plasmid

    #141257

    Purpose

    Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Has Service

    Cloning Grade DNA

    Insert

    NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-Syn-ChrimsonR-GFP

    Plasmid

    #58806

    Purpose

    AAV expression of ChrimsonR-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    Syn

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA5/FRT/TO V5

    Plasmid

    #19445

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    V5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V5 tag inserted to create new vector

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEF5-FRT-TagRFP-T-IFT88

    Plasmid

    #61684

    Purpose

    Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    IFT88 (Ift88 Mouse)
    

    Use

    Tags

    TagRFP.T

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Rabbit GFP Cav 2.2 pMT2

    Plasmid

    #58737

    Purpose

    expression of GFP tagged rabbit Cav2.2 in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

    Insert

    Cav2.2 (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAnc80L65

    Plasmid

    #68837

    Purpose

    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

    Depositor

    Luk Vandenberghe

    Article

    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

    Insert

    Ancestral AAV Capsid Anc80L65
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Rep2

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLemir9-124

    Plasmid

    #31779

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

    Plasmid

    #71707

    Purpose

    A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

    Depositor

    Giulio Draetta

    Article

    Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

    Insert

    humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
    

    Use

    CRISPR

    Tags

    3xFLAG and hGEM(1/110)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1-NORAD

    Plasmid

    #120383

    Purpose

    Expression vector for lncRNA NORAD

    Depositor

    Igor Ulitsky

    Article

    Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

    Insert

    NORAD (NORAD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGL-FLKif3A

    Plasmid

    #13742

    Depositor

    Linda Wordeman

    Article

    Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

    Insert

    mouse Kif3A (Kif3a Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLenti PGK V5-LUC Puro (w543-1)

    Plasmid

    #19360

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

    Plasmid

    #104495

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, and AAV1

    Insert

    jGCaMP7s
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pICH41780

    Plasmid

    #48019

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. pLentiPGK Puro DEST JNKKTRClover

    Plasmid

    #59151

    Purpose

    Lentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pRN111

    Plasmid

    #84463

    Purpose

    pJB38-NWMN29-30 + SarA_P1-mCherry-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-mCherry from pRN11
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pMBP-MS2

    Plasmid

    #65104

    Purpose

    can be used to isolate RNA binding complexes

    Depositor

    Josep Vilardell

    Article

    Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

    Insert

    MS2
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    double mutation (V75Q and A81G) that prevents oli…

    Promoter

    tac

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pEGFP-N1 alpha-actinin 1

    Plasmid

    #11908

    Depositor

    Carol Otey

    Article

    Edlund et al Cell Motil Cytoskeleton. 2001 Mar . 48(3

    Insert

    alpha-actinin 1 (ACTN1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge