162,632 results

1. pmScarlet3-alphaTubulin_C1

   Plasmid

   #189768

   Purpose

   Construct for labelling microtubules in mammalian cells with mScarlet3-alphatubulin

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-alphaTubulin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG PSAM4-5HT3 HC IRES EGFP

   Plasmid

   #119740

   Purpose

   Chemogenetic activator expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F, R420Q, R424D, R428A

   Promoter

   CAG

   Available since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3 HA eIF4GI (1-1599)

   Plasmid

   #45640

   Depositor

   Nahum Sonenberg

   Article

   Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

   Insert

   eIF4GI transcript variant 2 (EIF4G1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKSE401

   Plasmid

   #62202

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDA_867

   Plasmid

   #231109

   Purpose

   A>G base editor

   Depositor

   John Doench, David Root

   Insert

   ABE8e-nCas9-SpG-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. tau/pET29b

   Plasmid

   #16316

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

   Insert

   tau (MAPT Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-rab7 WT

   Plasmid

   #12605

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB7 (Homo sapiens) (RAB7A Human)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-TLR2-CFP

   Plasmid

   #13015

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 2 (TLR2 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB-tetO-NB

   Plasmid

   #197092

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-Ub

    Plasmid

    #11928

    Purpose

    Used for in vivo monitoring of ubiquitin localization.

    Depositor

    Nico Dantuma

    Article

    Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-Cas9-P2A-Puro

    Plasmid

    #110837

    Purpose

    Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 35S-VP16-GWY/pAM

    Plasmid

    #201234

    Purpose

    Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-VP16-GWY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-28a-mCherry-MED1-IDR

    Plasmid

    #194545

    Purpose

    Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    MED1 (MED1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV CAMKII PSAM4 GlyR IRES EGFP

    Plasmid

    #119744

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CamKII

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pY016 (pcDNA3.1-hLbCpf1)

    Plasmid

    #69988

    Purpose

    Expresses humanized LbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDule-tfmF A65V S158A

    Plasmid

    #85484

    Purpose

    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32V F108W Q109M I159M with 65Val and 158Ala

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-U6-gRNA-EF1a-EGFP-CAAX

    Plasmid

    #167936

    Purpose

    gRNA entry vector

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    U6-gRNA backbone
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHCMV-EcoEnv

    Plasmid

    #15802

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    MLV env (ecotropic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-45b-POLR2A-CTD-mCherry

    Plasmid

    #185011

    Purpose

    Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    POLR2A (Polr2a Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSmartBAC-S

    Plasmid

    #107268

    Purpose

    Broad host range bacterial artificial chromosome vector

    Depositor

    Mark Liles, Ron Godiska

    Article

    Nasrin et al J Nat Prod. 2018 Jun 22;81(6):1321-1332.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBMN FKBP(DD)-YFP

    Plasmid

    #31763

    Depositor

    Thomas Wandless

    Article

    Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

    Insert

    FKBP(DD)-YFP-HA (FKBP1A Human)
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    F36V, L106P on FKBP12

    Promoter

    LTR

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p37-2iDMD-LR

    Plasmid

    #88892

    Purpose

    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.

    Depositor

    Michele Calos

    Article

    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

    Inserts

    Dystrophin (DMD Human)
    luciferase
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-HA2-ROC1

    Plasmid

    #19897

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    RING finger protein 1 (ROC1) (RBX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

    Plasmid

    #190181

    Purpose

    Fluorescent reporter vector to visualize all of cell cycle phases.

    Depositor

    Toshiro Sato

    Article

    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

    Inserts

    iRFP
    hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
    TagBFP2
    hGeminin(1/110)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCS2+IMS-HyPer7

    Plasmid

    #136469

    Purpose

    Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    SMAC/DIABLO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSpCas9(BB)-2A-mCherry

    Plasmid

    #197420

    Purpose

    Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28 His6-mEGFP-D4H

    Plasmid

    #226373

    Purpose

    Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4H (pfoA Clostridium perfringens)
    

    Use

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 35S-GAL4BD-GWY/pAM

    Plasmid

    #201233

    Purpose

    Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GAL4BD-GWY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. His6-pAG-RTase

    Plasmid

    #214932

    Purpose

    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

    Depositor

    Chuan He

    Article

    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

    Insert

    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

    Promoter

    T7 promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Plasmid

    #179106

    Purpose

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBOB-synP-HTB

    Plasmid

    #30195

    Depositor

    Edward Callaway, Liqun Luo

    Article

    Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

    Insert

    histone-GFP-2A-TVA receptor-2A-rabies glycoprotein
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCRISPRa_all-in-one

    Plasmid

    #183695

    Purpose

    Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-Neo-Bam APC 1-1309

    Plasmid

    #16509

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    APC (APC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon 1309 truncation

    Promoter

    Available since

    April 30, 2008

    Availability

    Academic Institutions and Nonprofits only

34. p6sheLLr

    Plasmid

    #37318

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2005 Jul 5;337(2):365-72.

    Insert

    HPV6L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-Ef1a-Con/Fon-oScarlet

    Plasmid

    #137136

    Purpose

    Intersectional viral expression of oScarlet in cells expressing both Cre AND Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E95D

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 6xHis-MBP-BrCas12b-RFND

    Plasmid

    #195339

    Purpose

    Expresses Engineered BrCas12b in Bacteria

    Depositor

    Piyush Jain

    Article

    Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis and 6xHis, MBP, TEV cleavage site

    Expression

    Bacterial

    Mutation

    R160E, F208W, N524V, D868V

    Promoter

    T7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHIV-EGFP.Flag-EZH2

    Plasmid

    #220243

    Purpose

    Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p3xFLAG-CMV10-mApg16L

    Plasmid

    #24302

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

    Insert

    autophagy related genes 16L (Atg16l1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    V583M

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBabe-tetoff-mVenus-PICdc42

    Plasmid

    #91876

    Purpose

    PI(WT): Photo inhibitable (blue light sensitive)

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Insert

    Vav2 (VAV2 Human)
    

    Use

    Tags

    mVenus

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pL

    Plasmid

    #196286

    Purpose

    35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV L antigenome encoding codon-optimized L (RdRp)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge