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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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19,175 results

Showing: 5001 - 5050 of 19175 results

  4. pFC332

    Plasmid
    #87845
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87845-plasmid-map-sequence-id-418925

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  11. pAd-M3cherry

    Plasmid
    #61041
    Purpose
    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.
    Depositor
    Qiao Zhou
    Article
    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.
    Inserts
    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61041-plasmid-map-sequence-id-456476

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  13. pDB4581

    Plasmid
    #171124
    Purpose
    A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.
    Insert
    3_sAID
    Use
    Other
    Tags
    Expression
    Mutation
    non applicable
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171124-plasmid-map-sequence-id-336986

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  16. pKM461

    Plasmid
    #108320
    Purpose
    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB
    Depositor
    Kenan Murphy
    Article
    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-
    Inserts
    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    SacRB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108320-plasmid-map-sequence-id-210431

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  22. Luc-T20

    Plasmid
    #177941
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    177941-plasmid-map-sequence-id-348207

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  28. PB-TET

    Plasmid
    #20909
    Purpose
    piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)
    Depositor
    Andras Nagy
    Article
    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi
    Insert
    Gateway Destination Vector
    Use
    piggybac
    Tags
    IRES-bGeo
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    20909-plasmid-map-sequence-id-174009

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  35. tdTomato-CD9-10

    Plasmid
    #58076
    Purpose
    Localization: Tetraspanin/Membrane, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    CD9 (CD9 Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

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  44. mPA-GFP-LaminB1-10

    Plasmid
    #57141
    Purpose
    Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    LaminB1 (LMNB1 Human)
    Use
    Tags
    mPA-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. pCFD5_w

    Plasmid
    #112645
    Purpose
    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
    Depositor
    Michael Boutros
    Insert
    U6:3-tRNA-sgRNA expression cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112645-plasmid-map-sequence-id-217990

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Showing: 5001 - 5050 of 19175 results