165,307 results

1. pCDNA3-HA-Akt1

   Plasmid

   #73408

   Purpose

   Expresses human AKT1 with a N-terminus HA tag

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Tags

   HA tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV4a-Flag-c-Myc

   Plasmid

   #102625

   Purpose

   Expresses c-Myc in mammalian cells

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   MYC (MYC Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. neurod1:CaMPARI

   Plasmid

   #173479

   Purpose

   CaMPARI is driven by -5kb neurod1 promoter.

   Depositor

   John Rawls

   Article

   Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

   Insert

   CaMPARI
   

   Use

   Zebrafish expression

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSG5-BALF5

   Plasmid

   #72630

   Purpose

   Mammalian expression vector for Epstein-Barr virus DNA polymerase

   Depositor

   S. Diane Hayward

   Article

   Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

   Insert

   BALF5
   

   Expression

   Mammalian

   Mutation

   G249S

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pHR_EGFPligand

   Plasmid

   #79129

   Purpose

   Lentiviral vector for surface expressed EGFP (SFFV promoter)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   EGFP surface ligand
   

   Use

   Lentiviral

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. G608G lamin A minigene

   Plasmid

   #20292

   Depositor

   Tom Misteli

   Article

   Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

   Insert

   lamin A (LMNA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G606G:GGC>GGT Mutation causes aberrant spli…

   Available Since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

7. W118-1_hZBTB7B

   Plasmid

   #180380

   Purpose

   lentiviral transduction of human ZBTB7B gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   ZBTB7B (ZBTB7B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pROS10

   Plasmid

   #107924

   Purpose

   URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-FLAG-Sestrin1 isoform1

   Plasmid

   #72594

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN1 (SESN1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pACAGW-oChiEFtdTomato-AAV

    Plasmid

    #32846

    Purpose

    AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

    Depositor

    Rory McQuiston

    Article

    Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

    Insert

    oChiEF
    

    Use

    AAV

    Tags

    tdTomato

    Promoter

    CAGG

    Available Since

    March 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT4-CMV-GFP

    Plasmid

    #117046

    Purpose

    Optimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter

    Depositor

    Wolfgang Uckert

    Insert

    GFP
    

    Use

    Transposon

    Promoter

    CMV

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Luciferase-pcDNA3

    Plasmid

    #18964

    Depositor

    William Kaelin

    Article

    Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

    Insert

    Firefly Luciferase
    

    Expression

    Mammalian

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3-EF1A-CCNE1

    Plasmid

    #162553

    Purpose

    Overexpression of CCNE1

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of CCNE1 (CCNE1 Human)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. miRFP670nano-pcDNA

    Plasmid

    #127443

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti CMV Hygro DEST (w117-1)

    Plasmid

    #17454

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. VAMP2-SNAPtag

    Plasmid

    #107372

    Purpose

    Synaptic vesicle protein VAMP2 fused to the SNAPtag domain

    Depositor

    David Perrais

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAMP2 (Vamp2 Rat)
    

    Tags

    SNAPtag

    Expression

    Mammalian

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4.15_pSOX17

    Plasmid

    #195496

    Purpose

    Firefly luciferase promoter expression vector driven only by the promoter element of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    Promoter region of SOX17 (SOX17 Human)
    

    Tags

    luc2-hPEST

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-AKAP79-Dronpa

    Plasmid

    #182332

    Purpose

    AKAP79-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    AKAP79-Dronpa (AKAP5 Human)
    

    Tags

    Full-length AKAP79

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed

    Plasmid

    #157974

    Purpose

    Template plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9

    Depositor

    Carolyn McBride

    Article

    Zhao et al Nature. 2022 May;605(7911):706-712. doi:

    Insert

    Orco
    

    Expression

    Insect

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

    Plasmid

    #126049

    Purpose

    For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

    Depositor

    François Bachand

    Article

    Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

    Insert

    TurboID
    

    Use

    Pcr-based c-terminal tagging

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. EBV BGLF4 KD

    Plasmid

    #26692

    Depositor

    Robert Kalejta

    Article

    Kuny et al PLoS Pathog. 2010 . 6(9):.

    Insert

    BGLF4
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Kinase deficient mutant: Lysine 102 mutated to I…

    Available Since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

22. pENTR223-BGLF1

    Plasmid

    #195818

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BGLF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BGLF1
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pENTR223-BALF3

    Plasmid

    #195828

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BALF3

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BALF3
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV2-hNCOA6:V5

    Plasmid

    #63585

    Purpose

    A subunit of the Trithorax-related (Trr) methyltransferase complex.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

    Insert

    NCOA6 (NCOA6 Human)
    

    Tags

    6xHis and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA3-β2AR-μDpTT

    Plasmid

    #182326

    Purpose

    Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    beta2AR-μDronpa-TagRFP-T (ADRB2 Human)
    

    Tags

    Full-length human beta2 adrenergic receptor

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV CEPIA2mt

    Plasmid

    #58218

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA2mt
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V M339L

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRL092 pattB_cryaa:Venus_MCS

    Plasmid

    #213513

    Purpose

    attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pXP842

    Plasmid

    #46068

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    ADH2

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEST-CMV-N-mCherry

    Plasmid

    #123215

    Purpose

    N-terminal mCherry tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TFORF2854

    Plasmid

    #143384

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FEV (FEV Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid

    #220610

    Purpose

    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-KORD-P2A-ArgiNLS-AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pENTR223-BcLF1

    Plasmid

    #198581

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BcLF1
    

    Use

    Gateway entry vector

    Mutation

    None (wildtype)

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pENTR223-BXLF2

    Plasmid

    #195830

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BXLF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BXLF2 (BXLF2 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSG5-BBLF4

    Plasmid

    #72634

    Purpose

    Mammalian expression vector for Epstein-Barr virus DNA helicase

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BBLF4
    

    Expression

    Mammalian

    Mutation

    P5L

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

35. pRDA_052

    Plasmid

    #136474

    Purpose

    Lentiviral expression of AsCas12a gRNA

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    Puromycin resistance
    

    Use

    Lentiviral and Synthetic Biology

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TLS 5:hTLS-CHOP.pCDNA1

    Plasmid

    #21831

    Depositor

    David Ron

    Article

    Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

    Insert

    TLS-CHOP (FUS Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mApple-Centrin2-N-10

    Plasmid

    #54878

    Purpose

    Localization: Centrosomes, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Centrin2 (CETN2 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pUC118-mCtnnb1-FLIP-FlpE-Puro

    Plasmid

    #84541

    Purpose

    CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    mouse beta-catenin FLIP-FlpE - Puro targeting vector
    

    Use

    Cre/Lox and Mouse Targeting

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

39. pLU-CMV-Flag-hSesn2 WT

    Plasmid

    #111776

    Purpose

    Mammalian expression or 3rd generation lentivirus generation of hSesn2

    Depositor

    Jun Hee Lee

    Article

    Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

    Insert

    hSesn2 (SESN2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLC242-GFP-Sestrin2

    Plasmid

    #100519

    Purpose

    Expresses GFP tagged Sestrin2 in mammalian cells to make lentivirus

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    SESN2 (SESN2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge