163,682 results

1. pSCrhaB2

   Plasmid

   #113634

   Purpose

   An expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepacia

   Depositor

   Miguel Valvano

   Article

   Cardona et al Plasmid. 2005 Nov;54(3):219-28. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaB

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAWP78-PVCpnf1-16

   Plasmid

   #198271

   Purpose

   Unmodified pPVC; contains WT PVC structural/accessory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf1-16
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mScarletI3-6DG5-MAPPER

   Plasmid

   #222421

   Purpose

   To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   MAPPER
   

   Use

   Tags

   mScarletI3-6DG5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVpuro-CMV-N-3xFLAG

   Plasmid

   #123223

   Purpose

   N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPLICS PM-ER Long P2A

   Plasmid

   #164111

   Purpose

   Detect the long Plasma membrane-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP PM-ER Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ1661-sgMUC4-E3(F+E)

   Plasmid

   #51025

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

   Depositor

   Bo Huang, Stanley Qi

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   optimized sgRNA (MUC4 Mouse, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-Padron2

   Plasmid

   #172355

   Purpose

   Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

   Insert

   Padron2
   

   Use

   Tags

   6xHisTag (N terminal on backbone)

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. HyPer7.2DAAO-NLS

   Plasmid

   #168302

   Purpose

   Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

   Depositor

   Thomas Michel

   Article

   Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

   Insert

   HyPer7.2DAAO-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b His-p97

   Plasmid

   #169020

   Purpose

   Expresses His-p97 in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS3Clux

    Plasmid

    #55172

    Purpose

    lux-reporter vector, integration at sacA, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pdCas9-GFP

    Plasmid

    #181906

    Purpose

    Fluorescently tagged dead Cas9

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMS-II-dCas13-APO1-YTH

    Plasmid

    #204767

    Purpose

    GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    dCas13-NLS-APO1-YTH-HA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1alpha

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-puro-SNAP-FLAG-CD59

    Plasmid

    #50374

    Purpose

    Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SR alpha

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKIIa-DIO eHcKCR1 3.0-oScarlett-KV2.1

    Plasmid

    #195198

    Purpose

    all-optical silencing method

    Depositor

    Karl Deisseroth

    Article

    Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

    Insert

    eHcKCR1 3.0-oScarlet-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-GFP-MT2A

    Plasmid

    #11613

    Depositor

    Steven Johnson

    Insert

    metallothionein 2A (MT2A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lact-C2-GFP

    Plasmid

    #22852

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 Domain (MFGE8 Bovine)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBbS8c_ddCpf1_final

    Plasmid

    #212040

    Purpose

    Bacterial expression of the CRISPRi system for genome editing in E. coli

    Depositor

    Quanjiang Ji

    Article

    Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

    Insert

    ddCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    L-arabinose-inducible promoter PBAD

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-HA-Ubiquitin-KO

    Plasmid

    #17603

    Purpose

    Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    KO: all lysines mutated to arginines

    Promoter

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. NLS-Halo

    Plasmid

    #200197

    Purpose

    Expression for SV40 NLS

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    3X SV40 NLS-Halo
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pINDUCER dCas9-TET1CDMut

    Plasmid

    #101920

    Purpose

    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    dCas9-TET1CD Mut
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutated TET1 catalytic domain

    Promoter

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1-3'3xFlag

    Plasmid

    #208616

    Purpose

    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP

    Plasmid

    #230075

    Purpose

    Partial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

    Depositor

    Sumeet Pal Singh

    Article

    Hovhannisyan et al bioRxiv 2025.05.23.655316

    Insert

    lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    fabp10a

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAV.CAG.GLPLight1

    Plasmid

    #187467

    Purpose

    Expresses GLPLight1 in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

    Insert

    GLPLight1
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-DJ-N589X

    Plasmid

    #130878

    Purpose

    Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

    Depositor

    Prashant Mali

    Article

    Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

    Insert

    AAV-DJ cap N589X
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N589X

    Promoter

    Unknown

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR221-NPC1

    Plasmid

    #132295

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 14, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pLp_3050sNuc

    Plasmid

    #122030

    Purpose

    Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

    Depositor

    Geir Mathiesen, Vincent Eijsink

    Article

    Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

    Insert

    signal peptide Lp_3050
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    sppA

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. His-CL7-2NLS-SpyCas9(dS)-A22p3star

    Plasmid

    #222970

    Purpose

    Bacterial expression of A22p-fused SpyCas9 construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

    Insert

    His-CL7-2NLS-SpyCas9(dS)-A22p3
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTCF158 Human beta-2 microglobulin

    Plasmid

    #180457

    Purpose

    E. coli expression of class I MHC light chain

    Depositor

    John Altman

    Insert

    Human beta-2 microglobulin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lentiGuide-Hygro-dTomato

    Plasmid

    #99376

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-rab11 WT

    Plasmid

    #12674

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge