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20,197 results

Showing: 4681 - 4720 of 20197 results

  3. pJZC34

    Plasmid
    #62331
    Purpose
    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells
    Depositor
    Wendell Lim, Stanley Qi
    Article
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 2x MS2(wt+f6) binding module
    MCP-VP64
    Use
    Lentiviral
    Tags
    VP64
    Expression
    Mammalian
    Mutation
    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA
    Promoter
    CMV and U6
    Available Since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    62331-plasmid-map-sequence-id-102253

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  8. PtPuc3_diaCas9_sgRNA

    Plasmid
    #109219
    Purpose
    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum
    Depositor
    Per Winge
    Article
    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR and Synthetic Biology
    Promoter
    LHCF1 terminator, LHCF2 promoter, U6 3' regi…
    Available Since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    109219-plasmid-map-sequence-id-260727

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  15. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid
    #89180
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89180-plasmid-map-sequence-id-176308

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  19. pAC-LYC

    Plasmid
    #53270
    Purpose
    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53270-plasmid-map-sequence-id-487542

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  26. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Available Since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. mCherry-Lifeact-7

    Plasmid
    #54491
    Purpose
    Localization: Actin, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    mCherry-Lifeact
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54491-plasmid-map-sequence-id-227430

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  29. pAAV-SynaptoTAG2

    Plasmid
    #175275
    Purpose
    AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    EGFP-Synaptobrevin-2; tdTomato (Vamp2 )
    Use
    AAV
    Tags
    EGFP and tdTomato (P2A cleavage)
    Promoter
    Synapsin
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175275-plasmid-map-sequence-id-349084

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  34. mTLR4

    Plasmid
    #13085
    Depositor
    Ruslan Medzhitov
    Insert
    TLR4 (Tlr4 Mouse)
    Expression
    Mammalian
    Available Since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13085-plasmid-map-sequence-id-142564

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Showing: 4681 - 4720 of 20197 results