166,169 results

1. hBax C3-EGFP

   Plasmid

   #19741

   Depositor

   Richard Youle

   Article

   Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

   Insert

   Bax (BAX Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmTurquoise2-C1

   Plasmid

   #60560

   Purpose

   Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

   Plasmid

   #107941

   Purpose

   Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a (E174R/S542R/K548R)
   

   Tags

   3x HA and NLS (nucleoplasmin)

   Expression

   Mammalian

   Mutation

   E174R, S542R and K548R

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyCatcher003

   Plasmid

   #133447

   Purpose

   Expresses SpyCatcher003 in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDY1656 STITCHR Gluc AAVS1 payload

   Plasmid

   #234827

   Purpose

   Gluc exon 2 with 50bp AAVS1 homology arms

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

   Insert

   Gluc exon 2
   

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Dyn1-pmCherryN1

   Plasmid

   #27697

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Dynamin 1 (Dnm1 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pLenti.PGK.LifeAct-Ruby.W

   Plasmid

   #51009

   Purpose

   Lentivector that expresses LifeAct-Ruby from internal human PGK promoter

   Depositor

   Rusty Lansford

   Insert

   LifeAct-mRFPruby
   

   Use

   Lentiviral

   Tags

   LifeAct

   Expression

   Mammalian

   Promoter

   human PGK

   Available Since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX307-TMPRSS2-blast

   Plasmid

   #158458

   Purpose

   Constitutive lentiviral expression of human TMPRSS2.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

   Plasmid

   #163059

   Purpose

   mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   mito-roGFP2-Orp1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMV GFP Hygro (656-4)

    Plasmid

    #17446

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Lentiviral Prep

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLXSN p110 CUX1

    Plasmid

    #90471

    Purpose

    Retroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectively

    Depositor

    Alain Nepveu

    Article

    Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

    Insert

    CUX1 (amino acids 747-1505) (CUX1 Human)
    

    Use

    Retroviral

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    Moloney murine leukemia virus long terminal repeat

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. DTA in pCDEST2

    Plasmid

    #223705

    Purpose

    Mammalian expression vector encoding diptheria toxin A

    Depositor

    Rob Parton

    Article

    T. D. et al bioRxiv

    Insert

    Diphtheria toxin A
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1-pCMV-PfV-GS-Sapphire

    Plasmid

    #116933

    Purpose

    Mammalian vector for expression of the 40nm GEM

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Tags

    Sapphire

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. EC70188

    Plasmid

    #209449

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Promoter

    HvU3

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-EGFP-RhoA-Q63L

    Plasmid

    #12968

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA constitutively active (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q63L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-SEpHluorin

    Plasmid

    #32001

    Depositor

    Sergio Grinstein

    Article

    Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

    Insert

    mCherry
    

    Tags

    Superecliptic pHluorin

    Expression

    Mammalian

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-VPS13C-Cter

    Plasmid

    #232866

    Purpose

    Expresses the C-terminal fragments of VPS13C in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    the C-terminal fragments of VPS13C (VPS13C Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Casp3-myc

    Plasmid

    #11813

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-TOMM20-N-10

    Plasmid

    #55146

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-hSOX2

    Plasmid

    #17218

    Purpose

    Retroviral expression of human SOX2

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. pYPQ132A2.0

    Plasmid

    #99887

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. OPA1-isoform5

    Plasmid

    #70177

    Purpose

    Mammalian expression of hsOPA1 isoform 5

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 5 (OPA1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. lentiCRISPR v2-sgFSP1

    Plasmid

    #186026

    Purpose

    knock out FSP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Ferroptosis Suppressor Protein 1 (AIFM2 Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEGFP-NR2A

    Plasmid

    #17924

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    NMDAR2A (Grin2a Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCW-LIC-N8C_Fab3a

    Plasmid

    #203689

    Purpose

    Expresses N8C_Fab3a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab3a
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYPQ133A2.0

    Plasmid

    #99891

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRpHLuorin2-N1

    Plasmid

    #171716

    Purpose

    Ratiometric pHLuorin2 backbone (C terminal fusion)

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDae079 (eMutaT7[transition])

    Plasmid

    #187622

    Purpose

    Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Inserts

    TadA-8e-T7RNAP
    PmCDA1-T7RNAP
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pMB1-A

    Plasmid

    #190114

    Purpose

    Level 1 MoClo plasmid for A. baumannii

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMS2M-PH-gagpol-D64V

    Plasmid

    #166031

    Purpose

    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

    Insert

    MS2-Gag-Pol
    

    Use

    Lentiviral

    Mutation

    D64V mutation within the integrase within Pol

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET21d-myc-BirA

    Plasmid

    #109424

    Purpose

    Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    BirA (birA )
    

    Tags

    myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLJM1-FH-AGO2-WT

    Plasmid

    #91978

    Purpose

    Expresses FLAG-HA-AGO2 (WT)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-Syn-DIO-PinkyCaMP

    Plasmid

    #232857

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame)

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

    Plasmid

    #110559

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    rhaSR-PrhaBAD
    

    Expression

    Bacterial

    Promoter

    rhaSR-PrhaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Orai1 CFP

    Plasmid

    #19757

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 (ORAI1 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SpCas9-YE1-BE4max

    Plasmid

    #198546

    Purpose

    Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lenti_dCas9-KRAB-MeCP2

    Plasmid

    #122205

    Purpose

    Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Lentiviral

    Tags

    KRAB-MeCP2

    Expression

    Mammalian

    Mutation

    Cas9 (D10A, H840A)

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pC015 - dLwCas13a-NF

    Plasmid

    #91905

    Purpose

    Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    dLwCas13a
    

    Use

    CRISPR

    Tags

    NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

    Expression

    Mammalian

    Mutation

    R474A and R1046A

    Promoter

    CAG

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Douglas Kim, Loren Looger, GENIE Project

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLX208 CMV sTurboID (C)-HA-FRB-ERM

    Plasmid

    #153007

    Purpose

    Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (C) (G74)-ERM (Cb5)
    

    Use

    Lentiviral

    Tags

    FRB and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge