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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7,223 results for: ALP

Showing: 6051 - 6100 of 7223 results

  7. pSicoR-Ef1a-mCh

    Plasmid
    #31847
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Bruce Conklin
    Article
    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for shRNA; Ef1alpha for mCherry
    Available since
    Jan. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    31847-plasmid-map-sequence-id-35883

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  19. TFORF1329

    Plasmid
    #143789
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TFAP2A (TFAP2A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143789-plasmid-map-sequence-id-423575

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  20. pcDNA3.1.Gαq-LgB97

    Plasmid
    #134360
    Purpose
    Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαq-LgB97 (GNAQ Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134360-plasmid-map-sequence-id-306401

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  24. pMpGWB337

    Plasmid
    #68061
    Purpose
    Gateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoter
    Depositor
    Takayuki Kohchi
    Article
    Nishihama et al Plant Cell Physiol. 2015 Jul 6. pii: pcv
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Plant
    Mutation
    Promoter
    MpEF1alpha promoter
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68061-plasmid-map-sequence-id-124390

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  30. pD3sE_SC12

    Plasmid
    #219720
    Purpose
    DV3 E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV3 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219720-plasmid-map-sequence-id-433924

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  31. pCGN-ATF6 (1-373)m1

    Plasmid
    #27174
    Purpose
    For mammalian expression of the cytoplasmic domain of human ATF6 with mutations eliminating DNA binding and with an HA tag.
    Depositor
    Ron Prywes
    Article
    Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2
    Insert
    ATF6 (ATF6 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Contains only aa 1-373 Amino acids 315–317 are c…
    Promoter
    Available since
    Jan. 5, 2011
    Availability
    Academic Institutions and Nonprofits only

  35. pLV[Exp]-Neo-EF1A>Tet3G

    Plasmid
    #184379
    Purpose
    The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)
    Depositor
    Ori Bar-Nur
    Article
    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-
    Insert
    Tet3G
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    184379-plasmid-map-sequence-id-362511

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  36. pEGFP-ATF6-(S1P-)

    Plasmid
    #32956
    Purpose
    For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.
    Depositor
    Ron Prywes
    Article
    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-
    Insert
    ATF6 (ATF6 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S1Protease site mutation: R415A/R416A
    Promoter
    CMV
    Available since
    Feb. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    32956-plasmid-map-sequence-id-226826

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  37. FUT8-Dox

    Plasmid
    #124631
    Purpose
    Doxycycline-inducible FUT8 expression in mammalian cells
    Depositor
    Ron Weiss
    Article
    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038
    Inserts
    Alpha-(1,6)-fucosyltransferase
    Tetracycline repressor A3 mutant
    TagFP635
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight and hEF1a
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124631-plasmid-map-sequence-id-248079

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  44. TFORF2932

    Plasmid
    #144408
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TFAP2A (TFAP2A Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144408-plasmid-map-sequence-id-353338

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  47. pD4sE_SC12

    Plasmid
    #219721
    Purpose
    DV4 stable E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV4 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A259W, S262R, F279W, A280P, G29K, T33V, A2…
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219721-plasmid-map-sequence-id-433923

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  48. pD1sE_SC12

    Plasmid
    #219718
    Purpose
    DV1 E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV1 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A259W, T262R, F279W, A280P, S29K, T33V, A2…
    Promoter
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    219718-plasmid-map-sequence-id-433425

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  49. TFORF2934

    Plasmid
    #144410
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TFEB (TFEB Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144410-plasmid-map-sequence-id-407314

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Showing: 6051 - 6100 of 7223 results