20,197 results

Showing: 4961 - 5000 of 20197 results

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pMXs-p53

Plasmid

#22725

Depositor

Shinya Yamanaka

Article

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

Trp53 (Trp53 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pLX302

Plasmid

#25896

Purpose

3rd generation lentiviral Gateway destination vector. Puromycin selection, V5 tag.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5

Expression

Mammalian

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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14UAS PSD95:GFP 5UAS DSRedExpress

Plasmid

#74315

Purpose

Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish

Depositor

Martin Meyer, Stephen Smith

Article

Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

Inserts

zebrafish PSD95
DsRedExpress

Tags

EGFP

Mutation

Y599H and I793S (please see depositor comment bel…

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pDEST51-LRRK2-WT

Plasmid

#25080

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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CMV-GFP-NMHC II-B

Plasmid

#11348

Depositor

Robert Adelstein

Article

Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

Insert

NMHC II-B (MYH10 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 16, 2006

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-mIgkc

Plasmid

#127157

Purpose

Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Igkc (Igkc Mouse)

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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ND4.2-Right DdCBE-G1397-C- T1413I-GFP

Plasmid

#179686

Purpose

Expression of base editor in mammalian cells and for cell sorting

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR

Expression

Mammalian

Mutation

T1413I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

Plasmid

#50946

Purpose

An empty gRNA expression vector

Depositor

Kosuke Yusa

Article

Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

Insert

None

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCherry.90 alpha

Plasmid

#108222

Purpose

Mammalian expression vector for mCherry fusion to human Hsp90 alpha

Depositor

Didier Picard

Article

Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

Insert

mCherry-Hsp90a (HSP90AA1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-1x

Plasmid

#172281

Purpose

Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-1x

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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AA0250-mCherry-2a-hM4D-nrxn1a rev

Plasmid

#60544

Purpose

Co-expresses hM4Dnrxn and a red fluorescent label from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Inserts

mCherry
muscarinic receptor 4, axon targeted variant (CHRM4 Human)

Use

AAV and Cre/Lox

Tags

2xHA

Promoter

CAG

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

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synIRES_RC25

Plasmid

#189970

Purpose

Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

synIRES_RC25

Use

Cloning

Promoter

N/A

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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p23-DDX21 WT

Plasmid

#128803

Purpose

overexpression of DDX21 in human cells

Depositor

Ling-Ling Chen

Article

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 WT

Tags

flag

Expression

Mammalian

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-GFP11-pDEST-C

Plasmid

#118367

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-FLAG

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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mTurquoise2

Plasmid

#198196

Purpose

Spectral correction: Expression of mTurquoise2

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mTurquoise2

Expression

Mammalian

Mutation

A206K

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-MED17

Plasmid

#15410

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED17 (MED17 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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iRFP-PH-PLCdelta1

Plasmid

#66841

Purpose

expression of near-infrared PI(4,5)P2 biosensor

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

PH-PLCdelta1

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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TRE-Lepr_FL-IRES-eGFP

Plasmid

#196635

Purpose

Doxycycline inducible expression of full length mouse Lepr with eGFP marker

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

Lepr (Lepr Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-iSeroSnFR

Plasmid

#128484

Purpose

Fluorescent reporter for serotonin (mammalian expression, membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Tags

Ig-kappa leader, Myc, PDGFR + enhanced ER export,…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-kanMX6-P3nmt1-GFP

Plasmid

#39289

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-cFos-tTA-pA

Plasmid

#66794

Purpose

Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

cfos promoter linked to the tet activator gene

Use

AAV

Tags

no

Expression

Mammalian

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_NEUROD1_P2A_Hygro_Barcode

Plasmid

#120466

Purpose

Barcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROD1 (NEUROD1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB302

Plasmid

#113673

Purpose

expresses Cth SUMO protease in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Cth SUMO protease

Tags

10-His

Expression

Bacterial

Mutation

deleted amino acids 1-934

Promoter

T7

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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XRE-H2B-mScarlet-I

Plasmid

#182296

Purpose

Fluorescent reporter for AhR activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

mScarlet-I

Tags

m-Scarlet-I

Expression

Mammalian

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0

Plasmid

#153285

Purpose

Express the adenosine sensor GRAB_Ado1.0 in neurons

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Has Service

AAV1

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

hSyn

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGFP Cas

Plasmid

#50729

Purpose

mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Insert

Cas (Bcar1 Rat)

Tags

FLAG and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p5154

Plasmid

#175390

Purpose

High Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)

Depositor

Michael Wiles

Article

Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

Type

Empty backbone

Use

Mouse Targeting; Cloning vector, to be used to de…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-UbC-eGFP

Plasmid

#62518

Purpose

Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.

Depositor

Jae Lee, Pantelis Tsoulfas

Article

Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

hUbC

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-CA

Plasmid

#20960

Purpose

piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Gateway Destination Vector

Use

piggybac

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-Alpha-V-Integrin-25

Plasmid

#54866

Purpose

Localization: Focal Adhesions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

Alpha-V Integrin (ITGAV Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Syn-GFP

Plasmid

#58867

Purpose

AAV expression of GFP under the Syn promoter

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-PPARgamma2

Plasmid

#78768

Purpose

To overexpress PPARgamma2 in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPARG (Pparg Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
peGFP C3-SPG20

Plasmid

#218578

Purpose

Expresses GFP-tagged human SPG20 in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
DUSP6

Plasmid

#27975

Depositor

Igor Astsaturov

Article

Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

Insert

DUSP6 (DUSP6 Human)

Use

Lentiviral

Tags

HA and Myc

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158652

Purpose

making transgenics expressing a UAS driven eGFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Blast myc

Plasmid

#10674

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Insert

c-myc (MYC Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM246 - mosTI - unc-119 - MCS

Plasmid

#182343

Purpose

Empty cloning vector for mosTI(unc-119). MCS or Golden Gate (BsaI: tgcc - MCS - gagc)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV10-hAtg13

Plasmid

#22872

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only
pEG BacMam N term His8 eGFP 3C

Plasmid

#160684

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 eGFP 3C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc-CUL3

Plasmid

#19893

Depositor

Yue Xiong

Article

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

cullin 3 (CUL3 Human)

Tags

3xmyc

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge

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