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8,636 results

Showing: 6201 - 6250 of 8636 results
  1. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. GANLS

    Plasmid
    #50852
    Purpose
    Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.
    Depositor
    Insert
    ddGFP A
    Use
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pDULE-ABK

    Plasmid
    #49086
    Purpose
    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments
    Depositor
    Inserts
    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    GlnRS and lpp
    Available since
    Nov. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
  4. ePop

    Plasmid
    #50953
    Purpose
    Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations
    Depositor
    Inserts
    E lysis protein
    LuxI
    LuxR
    Use
    Tags
    Expression
    Mutation
    Early stop codon introduced in mutated luxR resul…
    Promoter
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pSico PGK puro

    Plasmid
    #11586
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.
    Depositor
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
  6. pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50455
    Purpose
    Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Has Service
    AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. EGFP-hCaM

    Plasmid
    #47602
    Depositor
    Insert
    CaM (CALM3 Human)
    Use
    Tags
    EGFP-HA-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 6201 - 6250 of 8636 results