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Showing: 6251 - 6300 of 12446 results

  4. Mouse NCKX5 (pcDNA3.1+)

    Plasmid
    #75213
    Purpose
    Mammalian expression of SLC24A5
    Depositor
    Jonathan Lytton
    Insert
    SLC24A5 (Slc24a5 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    75213-plasmid-map-sequence-id-142861

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  12. sgSf3b1(T1)

    Plasmid
    #90424
    Purpose
    induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    mus Sf3b1 gRNA (Sf3b1 Mouse)
    Use
    CRISPR; Guiderna encoding for gene editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90424-plasmid-map-sequence-id-190772

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  14. pCSC-ASCL1-IRES-Sox11

    Plasmid
    #182234
    Purpose
    To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189
    Depositor
    Chun-Li Zhang
    Article
    Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.
    Insert
    ASCL1-IRES-Sox11
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    March 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    182234-plasmid-map-sequence-id-357466

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  15. AAV-KrasG12S/sgKras/Cre

    Plasmid
    #99853
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99853-plasmid-map-sequence-id-193755

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  18. pSELECT-HA-mFOXO1-D256

    Plasmid
    #83379
    Purpose
    expresses mouse FOXO1 with truncating mutation at AA 256 in mammalian cells
    Depositor
    Steven Abcouwer
    Insert
    Forkhead box O1 (Foxo1 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    N256TAG (premature stop codon)
    Promoter
    EF-1a
    Available since
    Oct. 18, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pCMV-SUN2 GFP

    Plasmid
    #187684
    Purpose
    In vivo visualization of SUN2
    Depositor
    Mary Beckerle
    Insert
    SUN2 (Sun2 Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    187684-plasmid-map-sequence-id-371399

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  24. Cntn5.1-AP-His

    Plasmid
    #71943
    Purpose
    Expresses the extracellular region of the Contactin 5, isoform 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Cntn5.1 (Cntn5 Mouse)
    Use
    Tags
    AP-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71943-plasmid-map-sequence-id-136357

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  25. pBJ-mPar3

    Plasmid
    #53230
    Purpose
    mammalian expression of mouse Par3
    Depositor
    Shaun Coughlin
    Insert
    Par3 (F2rl2 Mouse)
    Use
    Tags
    FLAG and bovine prolactin signal sequence (MDSKGS…
    Expression
    Mammalian
    Mutation
    Deletion of aa1-15 (native signal peptide)
    Promoter
    Available since
    Aug. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. RCAS IkB K->A

    Plasmid
    #12408
    Depositor
    Inder Verma
    Insert
    IkB alpha K->A (Nfkbia Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Lysines 21, 22, and 67 are mutated to alanine. (T…
    Promoter
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only

  32. SRS-MTBD(89/82)-ABux

    Plasmid
    #22384
    Depositor
    Ron Vale
    Insert
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    Use
    Tags
    His and myc
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
    22384-plasmid-map-sequence-id-10292

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  33. mApple-Palladin-C-7

    Plasmid
    #54934
    Purpose
    Localization: Cytoskeleton, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Palladin
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    54934-plasmid-map-sequence-id-174354

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  38. pmirGLO-Smad5-1719

    Plasmid
    #49382
    Purpose
    Insert is a fragment of the Smad5 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Martine Roussel
    Article
    Murphy et al Cancer Res. 2013 Oct 21.
    Insert
    Smad5 (Smad5 Mouse, Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    49382-plasmid-map-sequence-id-73405

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  43. pCAG-DN-RFX

    Plasmid
    #52296
    Purpose
    Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag.
    Depositor
    Christopher A Walsh
    Article
    Bae et al Science. 2014 Feb 14;343(6172):764-8. do
    Insert
    Dominant negative RFX
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CAG promoter
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    52296-plasmid-map-sequence-id-80942

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  46. mCherry-KLC3-N-18

    Plasmid
    #62748
    Purpose
    Localization: Kinesin Light Chain , N1 Cloning Vector, Excitation:587, Emission:610
    Depositor
    Michael Davidson
    Insert
    KLC3
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  49. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156429
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    DELTA1-265
    Promoter
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156429-plasmid-map-sequence-id-310209

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Showing: 6251 - 6300 of 12446 results