Skip to main content
Addgene
Showing: 6651 - 6700 of 157653 results

  2. pCS2+mNeonGreen-C Cloning Vector

    Plasmid
    #128144
    Purpose
    To express a protein of interest fused to the C-terminus of mNeonGreen
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    mNeonGreen fusion
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128144-plasmid-map-sequence-id-248605

    Enlarge

  5. pCXLE-SOX2-17-p2a-KLF4

    Plasmid
    #193290
    Purpose
    episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    193290-plasmid-map-sequence-id-379665

    Enlarge

  6. mTurquoise2-Giantin

    Plasmid
    #129630
    Purpose
    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    mTurquoise2-Giantin
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    129630-plasmid-map-sequence-id-260212

    Enlarge

  8. pLentiRNAGuide_003

    Plasmid
    #192505
    Purpose
    For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:
    Insert
    hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192505-plasmid-map-sequence-id-375155

    Enlarge

  9. pcDNA3-Casp2-Flag

    Plasmid
    #11811
    Depositor
    Guy Salvesen
    Insert
    Caspase-2 (CASP2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 12, 2006
    Availability
    Academic Institutions and Nonprofits only
    11811-plasmid-map-sequence-id-299764

    Enlarge

  16. TFORF1322

    Plasmid
    #141808
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF10 (KLF10 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141808-plasmid-map-sequence-id-396950

    Enlarge

  23. pGEX4T-1-Optimized UBE1L

    Plasmid
    #165099
    Purpose
    ecoli optimized expression of GST-fusion of human Ube1L
    Depositor
    Arthur Haas
    Insert
    UbeE1L (UBA7 Human)
    Use
    Tags
    GST from plasmid.
    Expression
    Bacterial
    Mutation
    codon optimized for E. coli expression
    Promoter
    Available since
    March 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    165099-plasmid-map-sequence-id-327741

    Enlarge

  24. pCAG2-EGFP-C-IFT122

    Plasmid
    #218737
    Purpose
    Expresses C-terminally EGFP-tagged IFT122 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    IFT122 (IFT122 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218737-plasmid-map-sequence-id-434227

    Enlarge

  25. MSCV-Cre

    Plasmid
    #24064
    Purpose
    Retroviral coexpression of Cre and EGFP
    Depositor
    Dan Littman
    Insert
    Cre
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    MESV 5' LTR
    Available since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24064-plasmid-map-sequence-id-322907

    Enlarge

  28. pM1s3TsR

    Plasmid
    #137922
    Purpose
    E. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    mRFP1
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    137922-plasmid-map-sequence-id-323851

    Enlarge

  30. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140002-plasmid-map-sequence-id-275516

    Enlarge

  41. pH2B-cherryFP

    Plasmid
    #51001
    Purpose
    Expresses H2B-chFP
    Depositor
    Rusty Lansford
    Insert
    CherryFP
    Use
    Tags
    histone2B
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51001-plasmid-map-sequence-id-77770

    Enlarge

  46. pTOX8

    Plasmid
    #127453
    Purpose
    Encodes MqsR toxin and tsPurple chromoprotein. CAM-R
    Depositor
    Matthew Waldor
    Article
    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii
    Inserts
    mqsR toxin
    tsPurple chromoprotein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized from protein sequence in FPbase
    Promoter
    apFAB46 and pRha
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127453-plasmid-map-sequence-id-251148

    Enlarge

  47. CoV2-N-R203M

    Plasmid
    #177952
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (R203M) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R203M
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177952-plasmid-map-sequence-id-348187

    Enlarge

Showing: 6651 - 6700 of 157653 results