170,825 results
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Plasmid#31791DepositorAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only
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pLNHA-C1-HsSmaug2_Q
Plasmid#147285PurposeMammalian Expression of HsSmaug2DepositorInsertHsSmaug2 (SAMD4B Human)
ExpressionMammalianMutationone silent mutation compared to the seqeuence giv…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-24xGCN4_v4-kif18b-24xPP7
Plasmid#74928PurposeTranslation reporterDepositorInsert24xGCN4_v4-kif18b-24xPP7
ExpressionMammalianAvailable SinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
rat APOBEC1 E63A
Plasmid#112861Purposeplasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cellsDepositorArticleInsertAPOBEC1 (Apobec1 Rat)
ExpressionMammalianMutationchanged Glutamic acid 63 to AlaninePromoterCMV promoterAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDM030
Plasmid#216810PurposeVector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.DepositorTypeEmpty backboneUseCRISPR; Fungal expressionExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-G-Rac1
Plasmid#180581PurposeddGFP sensor of Rac1DepositorInsertddGFP sensor of Rac1
UseAAVAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAM1
Plasmid#60487PurposeThis plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genomeDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDY0663 ACTB atgRNA with v2 scaffold
Plasmid#179108PurposeACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffoldDepositorInsertACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
UseCRISPRAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-EcAvs4
Plasmid#188806PurposeCodon optimized EcAvs4 with C-terminal His tagDepositorInsertEcAvs4
TagsHisExpressionBacterialPromoterT7Available SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin K6C
Plasmid#86587PurposeBacterial expression of Ubiquitin K6C for cross linking studiesDepositorAvailable SinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSP64-H2B-GFP
Plasmid#135605PurposeExpression of H2B-GFP mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratusDepositorInsertH2B-GFP
UseIn vitro transcription (mrna synthesis)TagsGFPPromoterUnknownAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
RINS1
Plasmid#107290PurposePlasmid for the expression of the RINS1 sensorDepositorInsertRINS1
TagssfGFPExpressionMammalianPromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL2-GAL4-UAS-Luc
Plasmid#33020DepositorInsert6X GAL4-UAS
TagsLuciferaseAvailable SinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIVT-iCre
Plasmid#232773PurposeIVT template for making modRNA encoding iCreDepositorInsertiCre
UseCre/Lox; In vitro transcriptionAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapFLEX.(mem).iGlucoSnFR2.mIRFP670nano3
Plasmid#244102PurposePlasma membrane targeted expression of green glucose sensor with non-responsive mIRFPDepositorInsertiGlucoSnFR2.mIRFP670nano3
UseAAVTagsIgG kappa leader and mIRFP670nano3Available SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
DCLK1(Iso1)-NanoLuc Fusion Vector
Plasmid#237193PurposeExpress DCLK1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoterDepositorAvailable SinceSept. 16, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPB-CAG-NR5A1-FLAG
Plasmid#242227PurposeExpression of full-length human SF-1 using piggyBac transpositionDepositorAvailable SinceSept. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCherry-P2A-NTRv2-pA
Plasmid#241195PurposeMiddle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2DepositorInsertmCherry; NTR-v2
UseMini-goldenMutationTCGC linkerAvailable SinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Beta catenin-SmBiT Fusion Vector
Plasmid#237071PurposeExpress Beta catenin-SmBiT Fusion Protein in Mammalian Cells under a CMV promoterDepositorHas ServiceDNAInsertbeta catenin (CTNNB1 Human)
TagsSmBiTExpressionMammalianMutationNonePromoterCMVAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF3107
Plasmid#144583PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT15
Plasmid#223387PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.DepositorInsert2x35s-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRP.ABCA4 c.5461-10T
Plasmid#226553PurposeMinigene encoding the ABCA4 c.5461-10T mutationDepositorAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pAAV GFP
Plasmid#213685PurposeExpresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tagDepositorInsertTwin-Strep GFP
UseAAVTagsTwin-StrepPromotercytomegalovirus enhancer/chicken β-actin promoterAvailable SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CUG-IRES-mCherry
Plasmid#222110PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralMutationCUG start codonAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Stx5L(m55V)-GFP
Plasmid#209845PurposeExpresses Stx5 M55V mutant fused to GFP in mammalian cellsDepositorInsertSTX5 (STX5 Human)
TagseGFPExpressionMammalianMutationChanged Methionine 55 to Valine, causes loss of S…PromoterCMVAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LCAPCW
Plasmid#205646PurposeshRNAmir backbone for RNA interference, with mCherry reporterDepositorInsertmiR-AB
UseLentiviralPromoterhCMVAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex_307-ccdB-EBFP2
Plasmid#201137PurposepLEX_307 plasmid with EBFP2 markerDepositorTypeEmpty backboneExpressionMammalianPromoterEF-1αAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsUPF3biso2_I
Plasmid#146574PurposeMammalian Expression of HsUPF3biso2DepositorInsertHsUPF3biso2 (UPF3B Human)
ExpressionMammalianMutationone silent mutation T510C compared to the sequenc…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-pCUP1-2xphlO
Plasmid#191794PurposeYeast gene induction with copper promoterDepositorInsertTRP1 pCUP1 with 2 phlO sites
UseYeast genomic targetingAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-col10a1-KI-donor
Plasmid#184874PurposeDonor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repairDepositorInsertsCollagen10a1 5' homology arm
Collagen10a1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
eGFP
UseCRISPR and Cre/LoxAvailable SinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG_ToeRep_N09_trigger
Plasmid#132740PurposeT7 RNAP-driven expression of first-generation toehold repressor index 9 trigger RNADepositorInsertToeRep_N09_trigger
ExpressionBacterialAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_Ptet*_3WJrep_N19_switch
Plasmid#132739PurposeInducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoterDepositorInsertPtet*_3WJrep_N19_switch
ExpressionBacterialAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKDEL-mScarlet3-H (aka mYongHong)
Plasmid#239411PurposeExpresses mScarlet3-H (aka mYongHong) localized to ER in mammalian cellsDepositorInsertmScarlet3-H-KDEL
ExpressionMammalianMutationM163H in mScarlet3PromoterCMVAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1c-C1
Plasmid#131821PurposeExpresses HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
TagsHcRedExpressionMammalianMutationThis is the cytoplasmic mutant causing AML, where…Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-eYFP
Plasmid#117382PurposeAn AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEYFP
UseAAVExpressionMammalianPromoterhSyn1Available SinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
VPS13D^EGFP
Plasmid#174109PurposeThe insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.DepositorInsertCodon optimized VPS13D
TagsEGFPExpressionMammalianMutationWith an internal EGFP tag in aminoacid position 1…PromoterCMVAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
RAD21 C-tag CRISPR
Plasmid#140540PurposeRAD21 tagging CRISPRDepositorInsertRAD21 (RAD21 Human)
UseCRISPRAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH-nCas9-PBE
Plasmid#98163PurposeExpresses CRISPR base editor in riceDepositorInsertNLS-APOBEC1-XTEN-nCas9-UGI-NLS
UseRiceExpressionPlantPromoterUbiAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/p-bacteria
Plasmid#64325PurposeExpresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR; Tetracycline-inducibleTags3xFLAGExpressionBacterialMutationD10A, H840APromoterpLtetO-1Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCHC042 (∆phaCAB)
Plasmid#226208PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.DepositorInsertHomology arms for phaCAB
UseSynthetic BiologyAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSGKP-km
Plasmid#117233PurposeA sgRNA expression plasmid for genome editing in Klebsiella PneumoniaeDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-UFM1
Plasmid#134639Purposeexpression of HA-tagged human UFM1 wildtypeDepositorAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SFFV-DTAG-CTERM-GFP
Plasmid#185765PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGMutationnonePromoterSFFVAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSG5-SUV39H2-FL
Plasmid#89135Purposemammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regionsDepositorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
XLone-Cre
Plasmid#235216PurposeTet-On 3G-inducible PiggyBac system for expression of Cre in human pluripotent stem cells.DepositorInsertCre
UseCre/LoxExpressionMammalianAvailable SinceAug. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mTagBFP2 PuroR
Plasmid#101893PurposeLentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmTagBFP2PromoterCMV immediate earlyAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBMN(CMV-copGFP-Puro-SMAR)
Plasmid#80391Purposeexpression copGFP and Puromycine, with an S/MAR element for episomal maintainceDepositorInsertcopGFP-T2A-Puromycine
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-miniTurbo
Plasmid#124647PurposeExpresses Flag-miniTurbo fusion for proximity labelingDepositorTypeEmpty backboneTagsFlagExpressionMammalianPromoterCMVAvailable SinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mGreenLantern
Plasmid#161912PurposemGreenLantern fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertmGreenLantern
ExpressionMammalianPromoterCMVAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only